miR-497 inhibited proliferation, migration and invasion of thyroid papillary carcinoma cells by negatively regulating YAP1 expression

Abstract

PurposeThis article aimed to investigate the effect of miR-497 on thyroid papillary carcinoma.Materials and methodsmiR-497 expression was analyzed using The Cancer Genome Atlas. A total of 56 papillary thyroid carcinoma patients’ tumor tissues and normal tissues were collected. Nthy-ori 3-1 and K1 cells were cultured. K1 cells were also transfected. Quantitative real-time polymerase chain reaction and Western blot were used to detect miR-497 and yes-associated protein 1 (YAP1) expression. Luciferase reporter assay was performed. MTT and Transwell assay were conducted to measure cells’ proliferation, migration and invasion. Immunofluorescence detection was used to detect YAP1-positive cells.ResultsmiR-497 was downregulated, while YAP1 was upregulated in thyroid papillary carcinoma tissues and K1 cells (P<0.05). Compared with the negative control group, the OD495 value and the migrating and invasive cell number were significantly lower in miR-497 mimics group and significantly higher in miR-497 inhibitor group (P<0.05). YAP1 was the target gene of miR-497. Compared with blank group, the OD495 value and the migrating and invasive cell number were significantly lower in si-YAP1 group and significantly higher in miR-497 inhibitor group (P<0.05), while no significant difference was found between si-YAP1+inhibitors group and blank group in these indicators.ConclusionmiR-497 regulated the proliferation, migration and invasion of K1 cells by negatively regulating YAP1 expression.

H3K27ac‐activated LINC00519 promotes lung squamous cell carcinoma progression by targeting miR‐450b‐5p/miR‐515‐5p/YAP1 axis

“…For instance, YAP1 promotes the autophagy in sarcoma 39. YAP1 is reported to be overexpressed in thyroid papillary carcinoma 40. In our presented study, although KEGG pathway analysis showed the link between LINC00519 and hippo pathway, we found that LINC00519 cannot affect MTS1/2 but up‐regulated YAP1.…”

LINC00473 contributes to the radioresistance of esophageal squamous cell carcinoma by regulating microRNA‑497‑5p and cell division cycle 25A

“…miRs have thus been shown to play pivotal roles in the biological functions of cancer cells, such as differentiation, the stress response, proliferation, apoptosis and sensitivity to irradiation (19). Various miRs of the miR-15/16 family, including miR-15a-3p, miR-16-1-3p and miR-497, exhibit anticancer behaviors (20)(21)(22)(23)(24)(25)(26). For instance, miR-497 has been shown to suppress the malignant behavior of papillary thyroid cancer cells by reversely modulating the expression of Yes associated protein 1 (YAP1) (22); it has also been shown to inhibit the angiogenesis and metastasis of hepatocellular carcinoma by impeding the actions of vascular endothelial growth factor A and astrocyte elevated gene-1 (23).…”

Regulation of microRNA‑497 expression in human cancer (Review)

“…In NSCLC and HCC, miR-497 inhibits the proliferation of cancer cells by targeting YAP1 (105,106). In papillary thyroid carcinoma, miR-497 inhibits cancer cell proliferation and invasion by negatively regulating the expression of YAP1 (107).…”