miR-497-5p-RSPO2 axis inhibits cell growth and metastasis in glioblastoma

Abstract

Numerous studies have found a relationship between cancer formation and aberrant microRNA expression, however the biological significance of miR-497-5p in glioblastoma (GBM) is still unknown. Compared to normal brain glial cells, miR-497-5p expression in GBM tissues was substantially lower in our study. The microRNA miR-497-5p targets R-spondin 2 (RSPO2) only when it is present. RSPO2 silencing has the same effect on GBM cells as miR-497-5p silencing, as demonstrated before. Additional mechanistic investigations have shown that miR-497-5p suppresses the Wnt/β-catenin signaling pathway by targeting RSPO2 to reduce cell proliferation, migration, and invasion. A negative correlation was discovered between MiR-497-5p and RSPO2 in 37 of the GBM tumors studied. MiR-497-5p-RSPO2 axis controls Wnt/β-catenin signaling and plays a function in GBM carcinogenesis, suggesting that it may be a therapeutic target to reduce GBM growth, as shown by our research findings.

Modulating the Wnt Signaling Pathway in Wound Healing: The Potential of L-Carnosine, Zinc Sulfate, and B-Complex Vitamins Cocktail

“…These findings suggest that the cocktail might enhance the Wnt pathway by reducing the inhibitory effects of SFRP1, SFRP2, SFRP4, and MMP7 genes while simultaneously increasing the enhancing effect of the RSPO2 gene. RSPO2 is a Wnt agonist that enhances Wnt signaling by potentiating the interaction between Wnt ligands and Frizzled receptors (29,30). Research indicated that introducing recombinant human RSPO2 into a controlled environment led to a notable rise in the growth of fibroblasts.…”

The biological functions and related signaling pathways of SPON2

“…Xu Y et al also demonstrated that miR-493 exerts inhibitory effects on liver cancer development by suppressing the expression of SPON2, thereby attenuating the WNT signaling pathway (25). In their study, Kun Chen et al demonstrated that miR-497-5p effectively suppressed the expression of SPON2 in glioblastoma (GBM) tumors, thereby inhibiting the WNT/b-catenin signaling pathway and subsequently reducing cellular metastasis (7). Furthermore, Kang et al demonstrated a significant correlation between Notch signal transduction and SPON2 expression through cDNA microarrays and in vitro studies (16).…”

“…In their study, Kun Chen et al. demonstrated that miR-497-5p effectively suppressed the expression of SPON2 in glioblastoma (GBM) tumors, thereby inhibiting the WNT/β-catenin signaling pathway and subsequently reducing cellular metastasis ( 7 ). Furthermore, Kang et al.…”

“…Chen K et al. demonstrated that SPON2 facilitates GBM cellular metastasis by activating the WNT/β-catenin signaling pathway ( 7 ). Additionally, Liu S et al.…”

“…observed a negative correlation between the methylation status of SPON2 and its gene expression ( 6 ). Furthermore, the expression of SPON2 is associated with microRNAs (miRNAs) ( 7 ).…”