MiR-494 Inhibits the Proliferation, Migration and Invasion of Cervical Cancer Cells by Regulating LETMD1

Abstract

LETMD1 is a differentially expressed gene selected by scientists from cervical cancer (CC) tissues by RT-PCR technology. It has been confirmed that LETMD1 is overexpressed in many human malignant tumors, so it can be used as an early diagnostic marker for malignant tumors and as a target for gene therapy. The purpose of this article is to further explore the effectiveness of miR-494 in inhibiting the proliferation, migration and invasion of CC cells by regulating LETMD1, selecting 40 cases of CC admitted to a hospital from June 2015 to September 2018 Patients, tumor tissue specimens were taken from the primary tumor tissue of CC, and normal tissues near the cutting edge were collected as controls. Normal tissues were confirmed by pathology after surgery that they were not invaded by cancer tissues. The results of the study showed that the expression level of miR-494 increased by 15%, and the prognostic survival rate after surgery increased by 20%, depending on gender, age, tumor size, and tumor site. After high expression of miR-494 in CC patients, the vascular invasion of CC cells was reduced by 33%, and distant metastasis was reduced by 11%, and the survival time of patients was significantly prolonged. After the expression of LETMD1, the proportion of cancer cells decreased by 5%, the proportion of macrophages increased by 2%, and the dendritic cells increased by 3%.

JAK1 Is a Novel Target of Tumor- and Invasion-Suppressive microRNA 494-5p in Colorectal Cancer

“…In consequence, this complex regulation of different molecular targets could additionally explain the different, and in part opposing, functional changes in the investigated specific cancer models. Nevertheless, most recent reports dealing with miRs like miR-494 describe only one-to-one relationships between this miRNA and their individual target [ 25 , 26 , 107 , 108 , 109 , 110 , 111 ]. Certainly, our own present in vitro study can depict a small part of this regulatory network only.…”

The mechanisms and diagnostic potential of lncRNAs, miRNAs, and their related signaling pathways in cervical cancer

“…Another study found that miR-494 is positively correlated with the survival time of CC patients ( Wen et al, 2022 ). When miR-494 was used to regulate LETMD1 expression, the proliferation, differentiation, and migration rates of HeLa cell lines increased slowly over 5 days, while the proportion of cancer cells decreased by 5%, the proportion of macrophages increased by 2%, and the proportion of dendritic cells increased by 3% after the expression of LETMD1 ( Wen et al, 2022 ). In an in vitro experiment, Yang et al found that downregulation of miR-494 expression inhibited CC cell proliferation and growth by regulating the expression of target gene PTEN , suggesting a potentially significant role of miR-494 in tumorigenesis and the development of CC, and introducing a new perspective for understanding of the molecular mechanisms underlying CC progression ( Yang et al, 2015 ).…”

“…Liu et al found a negative relationship between the overall survival of CC patients and miR-92a-3p, and observed that miR-92a-3p is able to promote CC stem cell proliferation, invasion, and cell cycle transition (Liu et al, 2021b). Another study found that miR-494 is positively correlated with the survival time of CC patients (Wen et al, 2022). When miR-494 was used to regulate LETMD1 expression, the proliferation, differentiation, and migration rates of HeLa cell lines increased slowly over 5 days, while the proportion of cancer cells decreased by 5%, the proportion of macrophages increased by 2%, and the proportion of dendritic cells increased by 3% after the expression of LETMD1 (Wen et al, 2022).…”

MicroRNA‐494 regulates high glucose‐induced cardiomyocyte apoptosis and autophagy by PI3K/AKT/mTOR signalling pathway

“…Studies have confirmed that miR‐494 plays a key role in apoptosis, proliferation, tumorigenesis, metastasis, and other processes. A number of studies have shown that miR‐494 is abnormally expressed in many diseases, among which miR‐494 was down‐regulated to varying degrees in cervical cancer, 12 spinal cord injury, 13 liver ischaemia/reperfusion injury, 14 and septicaemia‐induced myocardial injury. 15 On the contrary, miR‐494 expression was up‐regulated in bladder cancer, 16 diabetes‐induced myocardial injury, 17 Parkinson's disease, 18 and aging, 19 thus affecting the disease process.…”