MiR-490-5p Suppresses Cell Proliferation and Invasion by Targeting BUB1 in Hepatocellular Carcinoma Cells

Abstract

Objective: To verify that miR-490-5p could influence hepatocellular carcinoma (HCC) cells' proliferation, invasion, cycle, and apoptosis by targeting BUB1. Methods: Quantitative real time-PCR (QRT-PCR) was used to determine the miR-490-5p expression. Immunohistochemistry, qRT-PCR, and Western blot were employed to detect BUB1 and transforming growth factor-beta (TGFβ/Smad) signaling-related proteins expression in hepatic tissues and cells. The luciferase assay was used to confirm the targeting relationship between miR-490-5p and BUB1. The Cell Counting Kit-8, colony formation, Transwell invasion, scratch healing assays, and flow cytometry analysis were conducted to evaluate HCC cells proliferation, invasion, migration, and apoptosis alteration after transfection. Results: In HCC tissues and cells, lower expression of miR-490-5p was detected, while BUB1 was overexpressed than controls. The upregulation of miR-490-5p inhibited BUB1 expression and the overexpression of miR-490-5p or the under-expression of BUB1 inhibited HCC cells proliferation, migration, invasion, and increased the apoptosis rate. Conclusion: MiR-490-5p could regulate TGFβ/Smad signaling pathways by inhibiting BUB1, which could then inhibit HCC cells proliferation, invasion, and migration as well as decrease cell viability and increase apoptosis.

Identification of the Differentially Expressed Genes of Muscle Growth and Intramuscular Fat Metabolism in the Development Stage of Yellow Broilers

“…Ten common DEGs related to cell proliferation (BUB1, CCNB3, CCNE2, CDC20, MCM2, and PLK1, which were mainly enriched in the cell cycle and oocyte meiosis pathways) and apoptosis (BIRC5, CTSK, LMNB1, and LMNB2) were screened out, and mRNA expression levels of all were significantly lower in breast muscle tissue throughout the development stage. As is widely known, BUB1, CCNB3, CCNE2, CDC20, MCM2, and PLK1 genes have a positive regulatory effect on the cell cycle [38][39][40][41][42], and BIRC5, CTSK, LMNB1, and LMNB2 genes promote cell apoptosis [43][44][45]. The number of skeletal muscle fibers is mainly determined at the embryonic stage, while occurrence of new skeletal muscle after birth is mainly due to activation, proliferation, and differentiation of satellite cells [9,10].…”

Bioinformatic Analysis Revealing Mitotic Spindle Assembly regulated NDC80 and MAD2L1 as Prognostic Biomarkers in Non-Small Cell Lung Cancer Development

“…It forms a heterotetramer complex with proteins SPC24, SPC25 and NUF2, and the complex has been known to involve in spindle assembly checkpoint signaling, detecting the unaligned chromosomes to assure the correct segregation of chromosomes. Aberrant expression of NDC80 has been reported in several other tumors [38][39][40][41][42] , for instance osteosarcoma, hepatocellulcar carcinoma, colorectal cancer and breast cancer, indicating its potential as a newly bio target.…”

“…Despite the barely known signaling pathways it participated in, MAD2L1 is shown to interact with CDC20 and BUB1B 43,44 , and correlate with aberrant development of salivary duct carcinoma 45 . BUB1, which is encoded by BUB1B, has been known as a checkpoint for proper chromosome segregation, the abnormal expression of BUB1 has been reported to associate with poor survival and metastasis in various tumors including colorectal cancer, gastric cancer, bladder cancer, hepatocellular carcinoma and so on [35][36][37][38] . In the study, using bioinformatic analysis, we con rmed the correlation between over expression of BUB1B and poor survival of NSCLC patients.…”

Screening and identification of key biomarkers and related transcription factors in ovarian cancer by integrated bioinformatics analysis

“…plays an important role in carcinogenesis and progression of various cancers, including gastric cancer (26), pancreatic ductal adenocarcinoma (27), glioblastoma (28), and hepatocellular carcinoma (29).…”