miR-485-5p alleviates Alzheimer’s disease progression by targeting PACS1

He, Chuan; Su, Caixia; Zhang, Wentong; Wan, Qi

doi:10.1515/tnsci-2020-0177

Cited by 17 publications

(9 citation statements)

References 41 publications

(47 reference statements)

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“…MiRNAs are reported to participate in various neurological diseases. 28,29 Accumulating findings have confirmed that miR-33-5p is associated with the pathogenesis and progression of neurological disorders. MiR-33-5p downregulation protects neuronal cells from oxygen-glucose deprivation (OGD)-mediated cell death.…”

Section: Discussionmentioning

confidence: 94%

MiR‐33‐5p alleviates spinal cord injury in rats and protects PC12 cells from lipopolysaccharide‐induced apoptosis

Rong²,

Zhang

2022

The Kaohsiung J of Med Scie

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MicroRNAs (miRNAs) exert critical effects in spinal cord injury (SCI). The miR‐33‐5p level is found to be lower in rats with SCI compared with that in control (untreated) and sham‐operated (laminectomy but no contusion) rats. Therefore, we investigated the biological functions of miR‐33‐5p and related mechanisms in SCI pathogenesis and development. An in vivo SCI model and a lipopolysaccharide (LPS)‐induced cell model of SCI were established. A downregulated level of miR‐33‐5p in experimental SCI and in LPS‐treated PC12 cells was revealed by reverse transcriptase‐quantitative polymerase chain reaction (RT‐qPCR). MiR‐33‐5p upregulation alleviated the leakage of the blood–spinal cord barrier (BSCB) induced by SCI and improved the neurological functions of SCI rats, as evidenced by the Basso, Beattie, and Bresnahan (BBB) scores and Evans blue staining. The regulatory relationship between miR‐33‐5p and Rps6kb1 was verified by luciferase reporter assays, which demonstrated that miR‐33‐5p bound to the Rps6kb1 3′UTR. Moreover, as MTT assays and flow cytometry showed, the suppressive effects of miR‐33‐5p upregulation on cell apoptosis were attenuated by Rps6kb1 upregulation. In conclusion, miR‐33‐5p ameliorates SCI in rats and inhibits the LPS‐induced apoptosis of PC12 cells.

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Section: Discussionmentioning

confidence: 94%

MiR‐33‐5p alleviates spinal cord injury in rats and protects PC12 cells from lipopolysaccharide‐induced apoptosis

Rong²,

Zhang

2022

The Kaohsiung J of Med Scie

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“…Both microarray and clustering analyses revealed that the majority of DECEmiRs were classified into clusters 1–2, suggesting that DECEmiRs in clusters 1–2 are significantly correlated with AD progression. Importantly, many DECEmiRs in clusters 1–2, including let-7f-5p and miR-485-5p, have been reported with neuroprotective and anti-inflammatory effects in various in vitro and in vivo models of AD, indicating the potential involvement of DECEmiRs in AD pathogenesis ( Han et al, 2018 ; He et al, 2021 ). Since miRNAs achieve their biological functions mainly through regulating the expression of their targets, the functional analysis of the predicted targets of DECEmiRs may unveil underlying mechanisms that modulate the progression of AD.…”

Section: Resultsmentioning

confidence: 99%

Circulating extracellular vesicle-containing microRNAs reveal potential pathogenesis of Alzheimer’s disease

Wang

Yuan

Ding

et al. 2022

Front. Cell. Neurosci.

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The pathogenesis of Alzheimer’s disease (AD) remains unknown till today, hindering the research and development of AD therapeutics and diagnostics. Circulating extracellular vesicles (EVs) can be utilized as a new window to spy upon AD pathogenesis. Altered microRNA profiles were noted in both the cerebrospinal fluid (CSF)- and blood-isolated EVs of AD patients, implying the outstanding potential of circulating EV-containing miRNAs (CEmiRs) to serve as important regulators in AD pathogenesis. Although several CEmiRs were found to play a part in AD, the association of globally altered miRNA profiles in patients’ serum-derived EVs with AD pathogenesis remains unclear. In this study, we first investigated the miRNA profile in serum-derived EVs from AD, mild cognitive impairment (MCI) patients, and healthy individuals. We observed differential expression patterns of CEmiRs and classified them into 10 clusters. We identified the predicted targets of these differentially expressed CEmiRs (DECEmiRs) and analyzed their biological functions and interactions. Our study revealed the temporal regulation of complex and precise signaling networks on AD pathogenesis, shedding light on the development of novel therapeutic strategies, including multi-target drug combination for AD treatment.

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“…The overexpression of miR-485-5p facilitated learning and memory capabilities (according to MWM and CFC tests), promoted pericyte viability, and suppressed Aβ40-induced pericyte apoptosis in APP/PS1 transgenic mice. Mechanistically, miR-485-5p directly targeted PACS1, a hallmark of AD, in pericytes [ 70 ]. As miR-485-5p was downregulated in the prefrontal cortex of patients with late-onset AD [ 75 ], miR-485-3p was overexpressed in the brain tissues, CSF, and plasma of patients with AD [ 71 ].…”

Section: Role Of Mirnas In Learning Memory and Cognitive Disordersmentioning

confidence: 99%

MicroRNAs in Learning and Memory and Their Impact on Alzheimer’s Disease

Wang

Tsai

2022

Biomedicines

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Learning and memory formation rely on the precise spatiotemporal regulation of gene expression, such as microRNA (miRNA)-associated silencing, to fine-tune gene expression for the induction and maintenance of synaptic plasticity. Much progress has been made in presenting direct evidence of miRNA regulation in learning and memory. Here, we summarize studies that have manipulated miRNA expression using various approaches in rodents, with changes in cognitive performance. Some of these are involved in well-known mechanisms, such as the CREB-dependent signaling pathway, and some of their roles are in fear- and stress-related disorders, particularly cognitive impairment. We also summarize extensive studies on miRNAs correlated with pathogenic tau and amyloid-β that drive the processes of Alzheimer’s disease (AD). Although altered miRNA profiles in human patients with AD and in mouse models have been well studied, little is known about their clinical applications and therapeutics. Studies on miRNAs as biomarkers still show inconsistencies, and more challenges need to be confronted in standardizing blood-based biomarkers for use in AD.

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miR-485-5p alleviates Alzheimer’s disease progression by targeting PACS1

Cited by 17 publications

References 41 publications

MiR‐33‐5p alleviates spinal cord injury in rats and protects PC12 cells from lipopolysaccharide‐induced apoptosis

MiR‐33‐5p alleviates spinal cord injury in rats and protects PC12 cells from lipopolysaccharide‐induced apoptosis

Circulating extracellular vesicle-containing microRNAs reveal potential pathogenesis of Alzheimer’s disease

MicroRNAs in Learning and Memory and Their Impact on Alzheimer’s Disease

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