miR-484/MAP2/c-Myc-positive regulatory loop in glioma promotes tumor-initiating properties through ERK1/2 signaling

Yi, Renhui; Feng, Jingyuan; Yang, Shaodong; Huang, Xiaoyu; Liao, Yuanyuan; Hu, Zheng; Luo, Muyun

doi:10.1007/s10735-018-9760-9

Cited by 33 publications

(17 citation statements)

References 31 publications

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“…Some researchers argue that MAP2 plays a dual role in the progression of cancer, which was studied in non-glioma tumors (Song et al, 2016). However, some authors assert that MAP2 may even be a positive marker of glioma, as shown in (Yi et al, 2018). However, in our work, we do not see a positive prognostic value of increased MAP2 expression for a patient.…”

Section: Fig 4 Normalized Expression Of Target Genes In Human Gliobcontrasting

confidence: 61%

Different localization of fluorescently labeled N- and C-termini of nucleolin variants in human glioblastoma cell culture

Panteleev

Pustogarov

Revishchin

et al. 2019

Preprint

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Nucleolus-oriented protein nucleolin plays a significant role in the life of a normal mammalian cell. However, nucleolin is also actively expressed in cells of malignant tumors. At the same time, its expression in different types of cancer is significantly increased compared with normal cells. It is interesting that nucleolin localization often varies in tumor cells, namely in the cytoplasm and on the cell membrane. This fact is considered to be a poor prognostic indicator. This work is devoted to the study of the distribution of nucleolin in human glioblastoma cells. Glioblastoma is one of the most aggressive malignant tumors with an absolutely unfavorable prognosis. These tumors have a high proliferative potential, but in addition they are often characterized by invasive properties. Research on lineage cells does not let to fully study these properties of glioblastoma, since lineage cells are very different from the actual tumor. In our study, we used two primary cell cultures of human glioblastoma with varying degrees of invasiveness of the original tumors. The main interest was directed at studying the localization of nucleolin and its correlation with the invariability of the N- and C-termini of the corresponding protein. Particular attention was paid to the significance of the unaltered C-terminus of nucleolin for its distribution in the cells of transplanted human glioblastoma cultures derived from patient tissues.The aim of this work is to find the relationship between the deformation of the N- or C-terminal sequences of nucleolin and its localization.We showed that in glioblastoma cells, with a high degree of invasion, nucleolin is found in the cytoplasm and close to the cell membrane, and the distribution of nucleolin with undeformed C and N-terminal does not match.

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Section: Fig 4 Normalized Expression Of Target Genes In Human Gliobcontrasting

confidence: 61%

Different localization of fluorescently labeled N- and C-termini of nucleolin variants in human glioblastoma cell culture

Panteleev

Pustogarov

Revishchin

et al. 2019

Preprint

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“…However, there are also some limitations in our study. Previous studies have demonstrated that miR-484 can reduce the expression of a series of oncogenes, such as ZEB1, SMAD2, etc., inhibit the epithelial-mesenchymal transition (EMT) of tumor cells, or regulate the extracellular signal-regulated kinase 1/2 signaling [16,34,38]. The precise molecular mechanisms underlying the role of miR-484 should be investigated in further studies.…”

Section: Discussionmentioning

confidence: 99%

Downregulation of miR-484 is associated with poor prognosis and tumor progression of gastric cancer

Yao

et al. 2020

Diagn Pathol

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Background: Gastric cancer is one of the most common cancers leading to high cancer mortality. MicroRNA-484 (miR-484) has been evaluated as a biomarker for various types of cancers. The subject of this study is to investigate the functional role of miR-484 in gastric cancer. Methods: The expression of miR-484 in gastric cancer was analyzed by quantitative real-time polymerase chain reaction (qRT-PCR) assay. Kaplan-Meier survival and Cox regression analyses were employed to explore the prognostic significance of miR-484 in gastric cancer. The functional role of miR-484 in gastric cancer was determined by CCK-8 and Transwell assays. Results: The results showed that miR-484 was significantly downregulated in gastric cancer tissues and cell lines. The downregulation of miR-484 was closely related to differentiation, lymph node metastasis, TNM stage, and poor prognosis. Cox regression analyses demonstrated that miR-484 was an independent prognosis indicator for gastric cancer patients. Additionally, the downregulation of miR-484 enhanced cell proliferation, migration, and invasion in gastric cancer cells. Conclusion: These data demonstrated that miR-484 can serve as a potential prognostic biomarker and therapeutic target for gastric cancer and it may be involved in the progression of gastric cancer.

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“…Previous studies have reported that miR-484 directly targets MAP2, which causes downstream ERK1/2 signal transduction to activate cancer stem cell characteristics and promote glioma tumorigenesis [27]. miR-484 can also inhibit Apf-1 expression to promote NSCLC proliferation and inhibit apoptosis in tumor cells to promote NSCLC development [28].…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-484 promotes cell migration and invasion in non-small cell lung cancer

Yang

Gong

et al. 2020

Preprint

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Background Lung cancer is one of the most common causes of cancer-related deaths in the world, and non-small cell lung cancer (NSCLC) accounts for 85% of it. Studies have reported that microRNA-484 (miR-484) plays an important regulatory role in carcinogenesis and cancer development. Methods 25 clinical NSCLC samples were collected for microRNA array. The funvtion of miR-484 was investigated through Transwell and Mitgration assays. The expression levers of epithelial-mesenchymal transition (EMT) related factors were assessed by Western blot. Results miR-484 was up-regulated in tissues from NSCLC patients relative to tumor-adjacent normal tissues. Knocking-down miR-484 expression in A549 cells significantly suppressed tumor cell invasion and migration, suppressed epithelial-mesenchymal transition (EMT) process by increasing the expression of E-cadherin, and decreasing the expression of N-cadherin, vimentin, and MMP2. Upregulation of miR-484 expression in BEAS-2B normal lung epithelial cells significantly promoted tumor cell invasion and migration, decreased E-cadherin expression levels, and increased N-cadherin, vimentin, and MMP2 expression levels Conclusion miR-484 can promote tumor cell invasion and migration in NSCLC and may be a new target for NSCLC treatment.

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miR-484/MAP2/c-Myc-positive regulatory loop in glioma promotes tumor-initiating properties through ERK1/2 signaling

Cited by 33 publications

References 31 publications

Different localization of fluorescently labeled N- and C-termini of nucleolin variants in human glioblastoma cell culture

Different localization of fluorescently labeled N- and C-termini of nucleolin variants in human glioblastoma cell culture

Downregulation of miR-484 is associated with poor prognosis and tumor progression of gastric cancer

MicroRNA-484 promotes cell migration and invasion in non-small cell lung cancer

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