MiR‐483–5p suppresses the proliferation of glioma cells via directly targeting ERK1

Abstract

Edited by Tamas DalmayKeyword: miR-483-5p cell proliferation ERK1 glioma a b s t r a c t MicroRNAs (miRNAs) exhibit tumor-specific expression signatures and play crucial roles in tumorigenesis by targeting oncogenes. Here, through analyzing the miRNA-array profiles of human glioblastoma tissues and the adjacent normal brain tissues, we found miR-483-5p was significantly down-regulated in gliomas, which was confirmed in both human glioma specimens and cell lines. The overexpression of miR-483-5p suppressed glioma cell proliferation and induced a G0/G1 arrest. In contrast, miR-483-5p inhibition promoted cell proliferation. Furthermore, by a dual-luciferase reporter assay and expression analysis, we identified extracellular signal-regulated kinase 1 (ERK1) as a direct target of miR-483-5p. ERK1 knockdown can block cell proliferation induced by miR-483-5p inhibition. Thus, our findings provide the first evidence that miR-483-5p can serve as a tumor suppressor in gliomas.

Dysregulation of MicroRNA-196b-5p and MicroRNA-375 in Gastric Cancer

“…The miR-483-5p gene is located in the chromosomal 11p15.5 region in the second intron of the IGF2 gene and expresses two mature forms (miR-483-5p and miR-483-3p) 42. To date, few studies have been conducted to reveal the function of miR-483-5p in gastric cancer, and its relation to carcinogenesis remains uncertain.…”

“…To date, few studies have been conducted to reveal the function of miR-483-5p in gastric cancer, and its relation to carcinogenesis remains uncertain. Wang et al42 reported that miR-483-5p is downregulated in human gliomas compared with normal brain tissues and that miR-483-5p can repress glioma cell proliferation by directly targeting extracellular signal regulated kinase 1, which is a core controller of cell proliferation and cell differentiation. Qiao et al43 suggested that miR-483-5p plays a role of an angiogenesis inhibitor.…”

RAS-MAPK pathway epigenetic activation in cancer: miRNAs in action

“…MiR-483-5p was significantly down-regulated in gliomas and directly targeted ERK1 (alias MAPK3) transcript [109]. MiR-124/214 cluster is down-regulated in squamous cell carcinoma.…”

miR-483-5p Promotes Invasion and Metastasis of Lung Adenocarcinoma by Targeting RhoGDI1 and ALCAM

“…Recently, miR-483 has been shown to be dysregulated and be associated with poorer disease-specific survival in some cancers (18)(19)(20)(21)(22). But the role of miR-483 in lung cancer metastasis and the molecular mechanisms by which miR-483 regulates lung cancer metastasis are not known.…”