miR-483-3p targets FGFR2 to inhibit cardiomyocyte pyroptosis NLRP3/Caspase-1/GSDMD signaling axis and reduce myocardial fibrosis

Abstract

Purpose In this study, the role of miR-483-3p in reducing myocardial fibrosis (MF) is investigated and the underlying molecular mechanism is further explored. Methods Firstly, six SD rats were randomly divided into sham surgery (Sham) group and model (ISO) group, and high-throughput miRNAs sequencing technology was used to sequence MF model rats. Forty male SD rats were randomly divided into Sham group, ISO group, blank transfection (AAV-NC) group, and overexpression (AAV-miR-483-3p) group, with 10 rats in each group. The MF model was established by tail vein injection of isoprenaline. RT-PCR, Western Blot and immunohistochemical staining were used to detect the expression of miR-483-3p, FGFR2 and cell pyroptosis. Bioinformatics software TargetScan was used to predict targets online and verify with diluciferase reporters; Detection of myocardial fibrosis using HE staining, Masson staining and Western Blot. Results Compared with the ISO group, the degree of MF decreased in the AAV-miR-483-3p group, and the expression of Collagen-1, FGFR2, NLRP3, Caspase-1, GSDMD and IL-1β in cardiomyocytes was significantly reduced. Diluciferase experiments confirmed that FGFR2 is the validated target gene of miR-483-3p. Conclusion miR-483-3p targets FGFR2 to inhibit cell pyroptosis and reduce the degree of MF, possibly via the NLRP3/Caspase-1/GSDMD signaling axis.