miR‑455‑5p promotes cell invasion and migration in breast cancer

Aili, Tuerxunjiang; Paizula, Xuelaiti; Ayoufu, Aisikeer

doi:10.3892/mmr.2017.8101

Cited by 9 publications

(9 citation statements)

References 27 publications

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“…In this study, we also validated the molecular mechanism of gga-miR-455-5p of most interest based on our results, both present and past, and in relation to the data present in the literature. It has been demonstrated that gga-miR-455-5p played an important role in various cancers and virus [33][34][35][36]. For example, miR-455-5p had the ability to promote cell invasion and migration in breast cancer [35].…”

Section: Discussionmentioning

confidence: 99%

“…It has been demonstrated that gga-miR-455-5p played an important role in various cancers and virus [33][34][35][36]. For example, miR-455-5p had the ability to promote cell invasion and migration in breast cancer [35]. In chickens, gga-miR-455-5p was downregulated in response to Newcastle disease virus (NDV) infection, and targeted SOCS3 to inhibit NDV replication [36].…”

Section: Discussionmentioning

confidence: 99%

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Analysis of miRNA Expression Profiling of RIP2 Knockdown in Chicken HD11 Cells When Infected with Avian Pathogenic E. coli (APEC)

Sun

Cao

Yang

et al. 2022

IJMS

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Colibacillosis is an acute and chronic avian disease caused by avian pathogenic E. coli (APEC). Previous studies have demonstrated that RIP2 plays a significant role in APEC infection. Moreover, increasing evidence indicates that microRNAs (miRNAs) are involved in host–pathogen interactions and the immune response. However, the role of miRNAs in the host against APEC infection remains unclear. Herein, we attempted to reveal new miRNAs potentially involved in the regulation of the immune and inflammatory response against APEC infection, with a particular focus on those possibly correlated with RIP2 expression, via miRNA-seq, RT-qPCR, Western blotting, dual-luciferase reporter assay, and CCK-8. The results showed that a total of 93 and 148 differentially expressed (DE) miRNAs were identified in the knockdown of RIP2 cells following APEC infection (shRIP2+APEC) vs. knockdown of RIP2 cells (shRIP2) and shRIP2 vs. wild-type cells (WT), respectively. Among those identified DE miRNAs, the biological function of gga-miR-455-5p was investigated. It was found that gga-miR-455-5p regulated by RIP2 was involved in the immune and inflammatory response against APEC infection via targeting of IRF2 to modulate the expression of type I interferons. Additionally, RIP2 could directly regulate the production of the type I interferons. Altogether, these findings highlighted the crucial role of miRNAs, especially gga-miR-455-5p, in host defense against APEC infection.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Analysis of miRNA Expression Profiling of RIP2 Knockdown in Chicken HD11 Cells When Infected with Avian Pathogenic E. coli (APEC)

Sun

Cao

Yang

et al. 2022

IJMS

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“…High expressed miR-455-5p was an independent prognostic factor for poor survival for breast cancer patients. In vitro experiments showed overexpression of miR-455-5p accelerated invasiveness and migration capabilities of breast cancer cells [27]. Li et al also found that miR-455 was intensively overexpressed in TNBC tissue and Abbreviations: CDKN1B, cyclin dependent kinase inhibitor 1B; ER, estrogen receptor; HER2, human epidermal growth factor 2; PAM50, research-based 50-gene prediction analysis of a microarray; PR, progesterone receptor; SMAD4, SMAD family member 4.…”

Section: Discussionmentioning

confidence: 99%

Identification of exosomal miR-455-5p and miR-1255a as therapeutic targets for breast cancer

et al. 2020

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Accumulated evidence has demonstrated exosomes of cancer cells carry microRNAs (miRNAs) to non-malignant cells to induce metastasis. The present study aimed to identify crucial exosomal miRNAs for breast cancer (BC) using microarray data (GSE83669 and GSE50429) from Gene Expression Omnibus database, including exosomal samples from human BC cells (MCF7, MDA-MB-231) and normal mammary epithelial cell line (MCF10, MCF-10A), as well as original cell samples. Differentially expressed miRNAs (DEMs) were identified using EdgeR package, and mRNA targets were predicted using miRWalk2 database. The target genes were overlapped with BC genes from Comparative Toxicogenomics Database (CTD) to construct BC-related interaction network. Potential functions were analyzed by DAVID. The expression of crucial miRNAs and target genes were confirmed in other microarray datasets or TCGA sequencing data. Their associations with survival and other clinical characteristics were validated by Kaplan–Meier plotter and LinkedOmics database. As a result, 9 and 8 DEMs were identified to be shared in two datasets for exosomal and original cells, respectively. Further comparison showed that miR-455-5p was specifically differentially expressed in exosomes, and miR-1255a was commonly expressed in exosomal and original cells samples. miR-455-5p could interact with CDKN1B to influence cell cycle process and miR-1255a could regulate SMAD4 to participate in TGF-β signaling pathway. High expressed miR-455-5p (basal-like) and miR-1255a (overall) were associated with poor overall survival, while the high expression of their target genes was associated with excellent overall, recurrence-free or distant metastasis-free survival. In conclusion, the present study preliminarily indicates that exosomal miR-455-5p and miR-1255a may be novel therapeutic targets for BC.

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“…Numerous microRNAs (miRNAs) have been confirmed to exert a tumor‐promoting effect in BC. For instance, miR‐455‐5p is upregulated in BC and accelerates cell invasion and migration 14 . MiR‐146a enhances the proliferation and metastasis of BC cells by suppressing NM2‐H1 15 .…”

Section: Introductionmentioning

confidence: 99%

“…For instance, miR‐455‐5p is upregulated in BC and accelerates cell invasion and migration. 14 MiR‐146a enhances the proliferation and metastasis of BC cells by suppressing NM2‐H1. 15 Previous research has reported that miR‐513a‐5p often functions as an onco‐miR in diverse cancers.…”

Section: Introductionmentioning

confidence: 99%

Long noncoding RNA ZBED3‐AS1 restrains breast cancer progression by targeting the microRNA‐513a‐5p/KLF6 axis

Zhang

et al. 2021

Thoracic Cancer

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Breast cancer (BC) is the most commonly occurring malignancy in women. This study aimed to investigate the functions of the long noncoding RNA ZBED3‐AS1 (ZBED3‐AS1) in BC and its molecular mechanisms. qRT‐PCR was conducted to access the expression of ZBED3‐AS1, microRNA‐513a‐5p (miR‐513a‐5p), and Kruppel like factor 6 (KLF6) in BC. Additionally, BC cell viability and proliferative capacity were measured by MTT and 5‐Ethynyl‐20‐deoxyuridine (EdU) assays. A transwell assay was used for evaluating BC cell migration and invasion. The interactions among ZBED3‐AS1, miR‐513a‐5p, and KLF6 in BC were confirmed by dual‐luciferase reporter assay. Furthermore, feedback approaches were performed to determine whether ZBED3‐AS1 influences BC cell behaviors by regulating the miR‐513a‐5p/KLF6 axis. The murine xenograft model was established to assess the effect of ZBED3‐AS1 on tumor growth. The expression of ZBED3‐AS1 and KLF6 was reduced, while miR‐513a‐5p expression was elevated in BC. ZBED3‐AS1 elevation attenuated the malignant behaviors of BC cells, including viability, proliferative capacity, migration, and invasion. Mechanical experiments revealed that ZBED3‐AS1 targeted miR‐513a‐5p, and miR‐513a‐5p targeted KLF6 in BC. Feedback approaches validated that miR‐513a‐5p overexpression or KLF6 depletion reversed the inhibitory effects of ZBED3‐AS1 upregulation on viability, proliferative capacity, migration, and invasion of BC cells. Furthermore, ZBED3‐AS1 elevation attenuated the tumor growth in the murine xenograft model. ZBED3‐AS1 hindered the malignant development of BC cells by regulating the miR‐513a‐5p/KLF6 axis, providing a novel therapeutic target in BC.

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miR‑455‑5p promotes cell invasion and migration in breast cancer

Cited by 9 publications

References 27 publications

Analysis of miRNA Expression Profiling of RIP2 Knockdown in Chicken HD11 Cells When Infected with Avian Pathogenic E. coli (APEC)

Analysis of miRNA Expression Profiling of RIP2 Knockdown in Chicken HD11 Cells When Infected with Avian Pathogenic E. coli (APEC)

Identification of exosomal miR-455-5p and miR-1255a as therapeutic targets for breast cancer

Long noncoding RNA ZBED3‐AS1 restrains breast cancer progression by targeting the microRNA‐513a‐5p/KLF6 axis

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