miR‑4458 directly targets IGF1R to inhibit cell proliferation and promote apoptosis in hemangioma

Abstract

Hemangiomas (HAs) are benign neoplasms of the vasculature. MicroRNA-4458 (miR-4458) has been reported to function as a tumor suppressor in multiple malignancies, but its biological function in HAs remains unknown. In the present study, the potential role of miR-4458 in HA-derived endothelial cells (HDECs) was investigated. Firstly, reverse-transcription-quantitative PCR analysis was used to confirm the expression of miR-4458 in HDECs following transfection with miR-4458 mimics or inhibitor. Subsequently, MTT and EdU assays were performed and subsequently determined that miR-4458 overexpression significantly inhibited proliferation, and knockdown promoted cell proliferation in HDECs. Flow cytometry analysis revealed that miR-4458 overexpression induced cell cycle arrest, whereas knockdown reversed G0/G1 phase arrest and apoptosis. Furthermore, insulin-like growth factor 1 receptor (IGF1R) was identified as a target of miR-4458. IGF1R knockdown enhanced the effects of miR-4458 on cell proliferation, cell cycle G0/G1 phase arrest and apoptosis in HDECs. Taken together, the results revealed that miR-4458 targeting of IGF1R may serve as a novel therapeutic strategy for treating patients with HAs.

Secreted insulin-like growth factor binding protein 5 functions as a tumor suppressor and chemosensitizer through inhibiting insulin-like growth factor 1 receptor/protein kinase B pathway in acute myeloid leukemia

“…Our study exactly confirmed that the phosphorylation of PI3K, AKT and IGF1R were attenuated in the cells that stably and ectopically expressed IGFBP5. PI3K/AKT pathway is involved in IGF1R-mediated apoptosis prevention and cell cycle distribution in hematopoietic cells [38] . Another study also confirmed that the deactivation of the IGF1R-mediated PI3K/AKT pathway with formononetin resulted in cell cycle arrest at G1/S transition in breast cancer cells [39] .…”

MiR-4458 Regulates Autophagy and Apoptosis By Targeting P53 In Chronic Obstructive Pulmonary Disease.

“…Zeng et al showed that the inhibition of miR-21 alleviated autophagy and apoptosis in mice and 16HBECs exposed to cigarette smoke [30]. MiR-4458, as a novel miRNA, has been reported to regulate apoptosis in hemangiomas [31] and breast cancer [13]. However, as far as we know, the function and mechanism of action of miR-4458 on autophagy and apoptosis in COPD has never been investigated.…”

Transcriptomic Signatures of End-Stage Human Dilated Cardiomyopathy Hearts with and without Left Ventricular Assist Device Support

“…miR-4458 is a relatively new miRNA identified in several carcinomas [46,47]. At the cellular level, miR-4458 was shown to suppress cell proliferation and promote apoptosis in breast cancer and haemangioma [47,48]. miR-4458 functions as a negative modulator in cardiac hypertrophy and inhibition of this miRNA exacerbates cardiac hypertrophy.…”