MiR-425-5p/RAB2B Promotes the Proliferation, Invasion and Migration of Glioma Cells

Abstract

Aim/Background: Glioma is a malignant brain tumor with the characteristics of rapid growth, diffuse invasion and therapeutic resistance. MicroRNAs (miRNAs) recently have be studied for the treatment of glioma. Here, we conducted cell-based experiments to analyze the role of miR-425-5p by targeting RAB2B in glioma though regulating the proliferation, invasion and migration of glioma cells. Methods: The qRT-PCR analysis detected the expression level of miR-425-5p in glioma cells. The transfection efficiency was verified by qRT-PCR. Cell viability, cell apoptosis, and the expression of cell cycle regulators were determined by CCK-8, flow cytometry and western blot analysis, respectively. And, the invasion and migration of glioma cells were assessed by wound-healing experiment and transwell assay. Result: Among five kinds of human glioma cell lines (U251, SHG44, LN229, T98G), the U251 cell line was chosen for the subsequent experiment. MiR-425-5p overexpression inhibited the proliferation, invasion and migration of glioma cells and promoted the glioma cells apoptosis. In addition, RAB2B was demonstrated to be a target of miR-129-5p. RAB2B inhibition could also inhibited the proliferation, invasion and migration of glioma cells and promoted the glioma cells apoptosis. Conclusion: Our findings suggested that miR-425-5p could inhibit the proliferation, invasion and migration, and promoted apoptosis of glioma cells by downregulation of RAB2B.

MicroRNA-425 induces apoptosis and suppresses migration and invasion of human cervical cancer cells by targeting RAB2B

“…In conclusion, the study elaborated the role of miR-425/RAR2B regulatory axis in cervical cancer and the results suggest miR-425 to be a viable therapeutic target against the human cervical cancer as has been elucidated in human glioma. 34 The main limitation of the study is that only one molecular target of miR-425 was studies in the present study. Another limitation of the present study is the small sample size, similar studies with large sample size need to be carried out.…”