miR-424/322 is downregulated in the semen of patients with severe DNA damage and may regulate sperm DNA damage

Zhao, Kai; Chen, Yaoping; Yang, Ruifeng; Bai, Yang; Li, Cuiling; Li, Honggang; Xiong, Chengliang

doi:10.1071/rd15052

Cited by 21 publications

(14 citation statements)

References 31 publications

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“…In an infertile knock-out mouse model, increase in unrepaired DNA breaks has been linked with reduced expression of miR-16 and miR-19b in testes [131]. Moreover, analysis of seminal plasmas from 94 infertile men with high sperm DNA fragmentation index has revealed significant downregulation of miR-424 in this group, implicating a role for miR-424 in repair of double-strand breaks during spermatogenesis [111].…”

Section: Seminal Fluidal Mirnas and Male Infertilitymentioning

confidence: 99%

The role of epigenetics in idiopathic male infertility

Güneş

Arslan

Hekim

et al. 2016

J Assist Reprod Genet

106

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Infertility is a complex disorder with multiple genetic and environmental causes. Although some specific mutations have been identified, other factors responsible for sperm defects remain largely unknown. Despite considerable efforts to identify the pathophysiology of the disease, we cannot explain the underlying mechanisms of approximately half of infertility cases. This study reviews current data on epigenetic regulation and idiopathic male infertility. Recent data have shown an association between epigenetic modifications and idiopathic infertility. In this regard, epigenetics has emerged as one of the promising research areas in understanding male infertility. Many studies have indicated that epigenetic modifications, including DNA methylation in imprinted and developmental genes, histone tail modifications and short non-coding RNAs in spermatozoa may have a role in idiopathic male infertility.

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Section: Seminal Fluidal Mirnas and Male Infertilitymentioning

confidence: 99%

The role of epigenetics in idiopathic male infertility

Güneş

Arslan

Hekim

et al. 2016

J Assist Reprod Genet

106

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“…miR- 322 plays an important role in cell proliferation, cell differentiation, diabetes and male infertility [18–20]. The role in inflammatory response is not exactly reported.…”

Section: Resultsmentioning

confidence: 99%

“…miR-322/424 is a member of miR-15/107 family, also known as miR-16 family [16,17]. It is involved in the regulation of cell proliferation, cell differentiation, diabetes and male infertility [15,18–20]. In our preliminary study, miR-322 was predicted to target several sites of inflammatory factors using the software programs.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-322 inhibits inflammatory cytokine expression and promotes cell proliferation in LPS-stimulated murine macrophages by targeting NF-κB1 (p50)

Zhang

Song

et al. 2017

Bioscience Reports

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Inflammation is the body’s normal self-protection mechanism to eliminate pathogens and resist pathogen invasion. The excessive inflammatory response may lead to inflammatory lesions. The mechanisms accounting for inflammation remain hazy. miRNAs have been proposed to have crucial effects on inflammation. In the present study, we reported that lipopolysaccharide (LPS)-stimulation increased the expression levels of inflammatory cytokines and the cell-cycle progression was suppressed in RAW264.7 cells. Meanwhile, the expression of miR-322 was significantly down-regulated after LPS treatment. Bioinformatics predictions revealed a potential binding site of miR-322 in 3′-UTR of NF-κB1 (p50) and it was further confirmed by luciferase assay. Moreover, both the mRNA and protein levels of NF-κB1 (p50) were down-regulated by miR-322 in RAW264.7 cells. Subsequently, we demonstrated that miR-322 mimics decrease in the expression levels of inflammatory cytokines and cell-cycle repression can be rescued following LPS treatment in RAW264.7 cells. The anti-inflammatory cytokines expression including IL-4 and IL-10 were significantly up-regulated. Furthermore, miR-322 could also promote RAW264.7 cells proliferation. These results demonstrate that miR-322 is a negative regulator of inflammatory response by targeting NF-κB1 (p50).

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“…The investigators reported that up-regulated miRNAs presented an enriched localization in introns, affecting relevant genes for spermatogenesis and the predicted targets of the miRNAs contained critical genes associated to infertility [22]. miRNAs can also be a predictor for sperm DNA damage, as demonstrated by Zhao et al (2015), where they showed that miR-424/322 is involved in sperm DNA damage, and that the dysregulation of this miRNA can induce DNA double-strand breaks during spermatogenesis [23].…”

Section: Mirnas As a Biomarker For Male Infertilitymentioning

confidence: 99%

“…Recently, it was demonstrated that dysregulation of miRNAs (miR-29 and miR-424) can induce DNA double-strand breaks during spermatogenesis -further highlighting the increasing role miRNAs have on sperm integrity and function [23].…”

Section: Sperm-specific Mirnas and Male Infertilitymentioning

confidence: 99%

Sperm-Specific MicroRNAs - Their Role and Function

Al-Gazi¹,

Carroll²

2016

GGS

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The sperm cell is highly specialised, with the sole purpose to fertilize an oocyte. Sperm assumes a simplistic structure, consisting of a head, which is mostly void of cytoplasm, a nucleus packed with DNA tightly wound around protamines; a midpiece housing the mitochondria necessary to fuel its locomotion, and a tail or principle piece that whips in motion to move the sperm towards the oocyte. However, despite the lack of cytoplasmic space, increasing studies in sperm biology have revealed that the sperm cell contains a myriad of molecular constituents that may play a role post-fertilization, influencing the oocyte and embryo development (figure 1). One such group of molecules are the small non-coding RNAs, which include the microRNAs (miRNAs). microRNAs miRNAs are small non-coding single-strand RNA molecules with sizes ranging from 17-26 nucleotides. The first miRNA to be discovered was lin-4 in the nematode, Caenorhabditis elegans. Lin-4 was detected with conserved complementary site on lin-14 and subsequently affected the larvae developmental timing by targeting mRNA and inhibiting its function [1]. Since then, miRNAs have emerged to play key roles in many biological functions through binding to 3'UTR (Untranslated Region) of specific mRNAs leading to either mRNA degradation or protein translation inhibition [2]. These miRNAs are involved in regulating cell functions including gene expression during development, differentiation, cell proliferation and apoptosis [3,4].In addition to having an influence in normal biological and developmental processes, miRNAs have been found to be involved in many pathogenic processes including Cardiovascular Diseases (CVD), cancer, inflammatory or autoimmune diseases, metabolic disorders and neurodegenerative disorders [5]. miRNA biogenesis miRNAs are encoded in the genome and transcribed by RNA polymerase II to form long primary miRNA transcripts (pri-miRNAs) that contain a cap structure at the 5' end and are poly-adenylated at the 3' end.The primary miRNAs are subsequently processed in the nucleus by Drosha to form shorter stem loop structures called pre curser-miRNAs or pre-miRNA (figure 2).These pre-miRNAs are then HSOA Journal of Genetics & Genomic Sciences Mini ReviewAbstract microRNAs (miRNAs) are small non-coding single-stranded molecules (containing about 22 nucleotides) that are widely found in many organisms, as varied as micro-organisms, plants, invertebrates and vertebrates. These miRNAs function in RNA silencing and post-transcriptional regulation of gene expression. miRNAs have been found to be involved in a number of biological and pathological processes, including sperm biology and infertility. This review will describe and discuss the role of miRNAs in terms of their role in sperm function, male infertility and gene regulation. a) The fertilising sperm introduces more than the paternal genome, the oocyte-activating factor (PLCζ), proteins from post-acrosomal, and perinuclear thecal proteins and centrioles; b) Sperm also contain a complex population of RNA, ...

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miR-424/322 is downregulated in the semen of patients with severe DNA damage and may regulate sperm DNA damage

Cited by 21 publications

References 31 publications

The role of epigenetics in idiopathic male infertility

The role of epigenetics in idiopathic male infertility

MicroRNA-322 inhibits inflammatory cytokine expression and promotes cell proliferation in LPS-stimulated murine macrophages by targeting NF-κB1 (p50)

Sperm-Specific MicroRNAs - Their Role and Function

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