miR-381-3p participates in chondrogenesis and cartilage degradation by alleviating collagen 2 expression and enhancing mmp13 expression

Hou, Changhe; Zhang, Z.; Yang, Zhengmeng; Wu, Puxun; Gu, Minjing; Liao, W.

doi:10.1016/j.joca.2014.02.593

Cited by 2 publications

(1 citation statement)

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“…34,35 miR381-3p is an important member of the microRNAs, and has been shown to be involved in the regulation of a variety of human diseases, such as intestinal ischemia-reperfusion injury and chondrogenesis and cartilage degradation. 36,37 However, there are few records Compared with the NC-mimic group, 143B and HOS cells of the miR381-3p-mimic group had much lower CCND1 mRNA and protein expression. (E) Obviously lower CCND1 protein expression occurred in 143B and HOS cells of the siFLVCR-AS1 group than the siCtrl group.…”

Section: Discussionmentioning

confidence: 99%

<p>lncRNA FLVCR-AS1 promotes osteosarcoma growth by targeting miR381-3p/CCND1</p>

Yang¹,

He²,

Duan³

et al. 2020

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Purpose: This article reports on FLVCR-AS1 effects on osteosarcoma (OS) growth. Methods: Tumor tissue and adjacent normal tissue of 48 OS patients were collected. HOS and 143B cells were transfected. Gene expression was examined with qRT-PCR and Western blot. CCK8 assays and cell cloning was performed to measure cell proliferation. Cell cycle and apoptosis were assessed. Luciferase-reporter gene assays and RNA pull-down tests were used to detect targeting relationships between genes. Results: Prominently higher FLVCR-AS1 expression was found in OS tissue and cells, and was associated with poor prognosis (P<0.05, P<0.01, or P<0.001). Compared with the siCtrl group, 143B and HOS cells of the siFLVCR-AS1 group had significantly lower OD 450 values and clone numbers and obviously higher percentages of cells in the G 1 phase and apoptosis (P<0.01 or P<0.001). miR381-3p expression was directly inhibited by FLVCR-AS1, and CCND1 expression was directly suppressed by miR381-3p. Compared with the FLVCR-AS1 group, 143B cells of the FLVCR-AS1 + miR381-3p mimic group and FLVCR-AS1 + siCCND1 group showed remarkably lower OD 450 values and clone numbers obviously higher apoptosis and percentage of cells in the G 1 phase (P<0.05, P<0.01, or P<0.001). Conclusion: FLVCR-AS1 promoted OS growth by upregulating CCND1 expression via downregulation of miR381-3p.

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