miR-375 Affects the Proliferation, Invasion, and Apoptosis of HPV16-Positive Human Cervical Cancer Cells by Targeting IGF-1R

Xiao, Yu; Zhao, Weihong; Yang, Xin; Wang, Zhilian; Hao, Min

doi:10.1097/igc.0000000000000711

Cited by 28 publications

(22 citation statements)

References 29 publications

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“…3A. Although IGF-1R has been identified as a direct target of miR-375 in laryngeal squamous cell cancer and cervical cancer [18, 19], whether miR-375 targets IGF-1R to function in OSCC cells is unclear. First, the dual-luciferase reporter assay was performed and the luciferase activity was determined (Fig.…”

Section: Resultsmentioning

confidence: 99%

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MicroRNA-375 Inhibits Growth and Enhances Radiosensitivity in Oral Squamous Cell Carcinoma by Targeting Insulin Like Growth Factor 1 Receptor

Zhang

Hou

et al. 2017

Cell Physiol Biochem

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Background: MicroRNAs (miRNAs) have emerged as key players in various human biological processes, including tumorigenesis. Here, we investigated the roles of miR-375 in the pathogenesis of oral squamous cell carcinoma (OSCC). Methods: We performed quantitative real-time PCR (qRT-PCR) to detect miR-375 expression in OSCC tissues and corresponding normal oral epithelial tissues and analyze the correlation of miR-375 expression with OSCC metastasis and patient’s survival. Then, the effects of miR-375 expression on proliferation, cell cycle, apoptosis and radiosensitivity in OSCC cells were determined by using MTT, flow cytometry and clonogenic survival assays. A dual-luciferase reporter assay was performed to test whether miR-375 binds to the 3’-untranslated region (3’-UTR) of target mRNA. Results: The expression level of miR-375 in OSCC tissues was significantly lower than that in normal oral epithelial tissues, and low miR-375 expression was correlated with higher incidence of lymph node metastasis and poor survival of OSCC patients. Upregulation of miR-375 significantly inhibits growth, induces cell cycle arrest in G₀/G₁ phase, increases apoptosis and enhances radiosensitivity in OSCC cells. Analysis of luciferase activity demonstrated that miR-375 binds to the 3’-UTR of insulin like growth factor 1 receptor (IGF-1R). Small interfering RNA (shRNA)-mediated IGF-1R knockdown mimics the effects of miR-375 upregulation, while overexpression of IGF-1R partially reverses those effects in OSCC cells. Conclusion: It was obviously demonstrated that miRNA-375 inhibits growth and enhances radiosensitivity in OSCC cells by targeting IGF-1R, suggesting that miR-375 may be a potential therapeutic target for OSCC patients.

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Section: Resultsmentioning

confidence: 99%

“…By bioinformatics, IGF-1R gene was identified as a potential target of miR-375. In laryngeal squamous cell cancer and cervical cancer, IGF-1R has been testified as a functional target of miR-375 [18, 19]. IGF-1R belongs to the insulin receptor (IR) family which includes the IR, IGF-1R, IGF-1R/IR, and IGF-2R.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-375 Inhibits Growth and Enhances Radiosensitivity in Oral Squamous Cell Carcinoma by Targeting Insulin Like Growth Factor 1 Receptor

Zhang

Hou

et al. 2017

Cell Physiol Biochem

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“…9,10 Some other miRNAs, such as miR-139, miR-634, miR-375 and miR-125, were also recently found to be dysregulating in cervical cancer. [11][12][13][14] They modulated behaviors of cervical cancer cells, including proliferation, apoptosis, invasion, radio sensitivity and drug sensitivity, via posttranscriptional suppression of oncogenes Nin one binding protein (NOB1), mammalian target of rapamycin complex 1 (mTOR), signal transducer and activator of transcription 3 (STAT3), and insulin-like growth factor 1 receptor (IGF-1R).…”

Section: Introductionmentioning

confidence: 99%

Retracted Article: RNA-sequencing identified miR-3681 as a negative regulator in the proliferation and migration of cervical cancer cells via the posttranscriptional suppression of HGFR

et al. 2019

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“…MicroRNAs (miRNAs) are a class of endogenous and small noncoding RNAs with 21–23 ribonucleotides, which can induce mRNA degradation or act as repressors of translation through directly binding to a target site in the 3′‐untranslated regions of their target genes. Existing studies found that miRNA mediated gene regulation involved in multiple pathological processes including cell proliferation, differentiation, migration, survival, and tumorigenesis . Recent evidence has shown that miRNAs also plays a vital role in chemotherapeutic resistance.…”

Section: Introductionmentioning

confidence: 99%

Up‐regulation of miR‐497 confers resistance to temozolomide in human glioma cells by targeting mTOR/Bcl‐2

Zhu

Zeng

et al. 2017

Cancer Medicine

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The occurrence of an inherent or acquired resistance to temozolomide (TMZ) is a major burden for patients suffering from glioma. Recently, studies have demonstrated that microRNAs play an important role in the regulation of tumor properties in cancers. However, whether miR‐497 contributes to glioma resistance to chemotherapy is not fully understood. In this study, we showed that the expression of miR‐497 was markedly up‐regulated in TMZ‐resistant glioma cells; high miR‐497 expression level was associated with TMZ‐resistant phenotype of glioma cells. The down‐regulation of miR‐497 in glioma cells enhanced the apoptosis‐induction and growth inhibition effects of TMZ both in vitro and in vivo, whereas promotion of miR‐497 increased the chemosensitization of glioma cells to TMZ. The increased level of miR‐497 in TMZ‐resistant glioma cells was concurrent with the up‐regulation of insulin‐like growth factor 1 receptor (IGF1R)/insulin receptor substrate 1 (IRS1) pathway‐related proteins, that is, IGF1R, IRS1, mammalian target of rapamycin (mTOR), and Bcl‐2. In addition, the knockdown of mTOR and Bcl‐2 reduced the tolerance of glioma cells to TMZ. Our results demonstrated that overexpression of miR‐497 is significantly correlated with TMZ resistance in glioma cells by regulating the IGF1R/IRS1 pathway. Therefore, miR‐497 may be used as a new target for treatment of chemotherapy‐resistant glioma.

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miR-375 Affects the Proliferation, Invasion, and Apoptosis of HPV16-Positive Human Cervical Cancer Cells by Targeting IGF-1R

Abstract: miR-375 plays an important role in the tumorigenesis and development of cervical cancer. IGF-1R might represent a target gene of miR-375 in cervical cancer.

Cited by 28 publications

References 29 publications

MicroRNA-375 Inhibits Growth and Enhances Radiosensitivity in Oral Squamous Cell Carcinoma by Targeting Insulin Like Growth Factor 1 Receptor

MicroRNA-375 Inhibits Growth and Enhances Radiosensitivity in Oral Squamous Cell Carcinoma by Targeting Insulin Like Growth Factor 1 Receptor

Retracted Article: RNA-sequencing identified miR-3681 as a negative regulator in the proliferation and migration of cervical cancer cells via the posttranscriptional suppression of HGFR

Up‐regulation of miR‐497 confers resistance to temozolomide in human glioma cells by targeting mTOR/Bcl‐2

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