MiR‐372‐3p promotes cell growth and metastasis by targeting <i><scp>FGF</scp>9</i> in lung squamous cell carcinoma

Wang, Qing; Liu, Siyang; Zhao, Xiaowen; Wang, Yuan; Tian, Dean; Jiang, Wenjun

doi:10.1002/cam4.1026

Cited by 55 publications

(58 citation statements)

References 40 publications

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“…Traditional treatments for LSCC are radiotherapy, chemotherapy, and lobectomy, which are harmful to patients and have a high risk of side effects and recurrence [27]. Our study confirmed the binding relationship between miR-30a-5p and FOXD1 and found that miR-30a-5p may inhibit the proliferation and migration of LSCC cells by inhibiting FOXD1 expression, which provides new insight into the treatment of LSCC.…”

Section: Discussionsupporting

confidence: 78%

miR-30a-5p Inhibits Proliferation and Migration of Lung Squamous Cell Carcinoma Cells by Targeting FOXD1

Chen

Tang

et al. 2020

BioMed Research International

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Objective. To investigate the mechanism of miR-30a-5p inhibiting proliferation and migration of lung squamous cell carcinoma (LSCC) cells by targeting FOXD1. Methods. Bioinformatics was used to analyze differentially expressed genes in the TCGA_LUSC database. qRT-PCR was used to detect the expression levels of miR-30a-5p and FOXD1 in human normal lung epithelial cell line and human LSCC cell lines. The protein expression of FOXD1 was detected by western blot. The cell viability and colony formation abilities were examined by CCK-8 and colony formation assays, respectively. Wound healing and Transwell assays were performed to examine the migration and invasion abilities of cells. The targeted binding sites of miR-30a-5p and FOXD1 were predicted by bioinformatics, and dual luciferase assay was used to verify the targeted binding relationship between miR-30a-5p and FOXD1. Result. miR-30a-5p was downregulated in LSCC tissues and cells, while FOXD1 was highly expressed. Overexpression of miR-30a-5p or silencing FOXD1 inhibited cell viability, colony formation ability, migration, and invasion of LSCC cells. miR-30a-5p inhibited the proliferation and migration of LSCC cells by downregulating the expression of FOXD1. Conclusion. miR-30a-5p can downregulate the expression of FOXD1 and inhibit the proliferation and migration of LSCC.

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Section: Discussionsupporting

confidence: 78%

miR-30a-5p Inhibits Proliferation and Migration of Lung Squamous Cell Carcinoma Cells by Targeting FOXD1

Chen

Tang

et al. 2020

BioMed Research International

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“…It has been demonstrated that miR-372-3p regulates cell growth and metastasis by targeting FGF9 in lung squamous cell carcinoma. (Wang et al 2017b) Another report revealed that miR-372-3p inhibited the growth and metastasis of osteosarcoma cells by targeting FXYD6. (Xu et al 2018) HULC could act as an endogenous sponge antagomir to downregulate miR-372 expression in hepatic cell carcinoma.…”

Section: Introductionmentioning

confidence: 99%

LncRNA HULC induces the progression of osteosarcoma by regulating the miR-372-3p/HMGB1 signalling axis

Liu

Zhou

et al. 2020

Mol Med

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Background: Osteosarcoma is a malignancy that normally affects children, adolescents, and young adults. Although accumulating evidence has demonstrated the importance of HULC in osteosarcoma, little is reported about its functional roles and molecular mechanisms. Methods: The expression of HULC and miR-372-3p in osteosarcoma tissues was quantified by qRT-PCR. The regulatory roles of HULC and miR-372-3p on cell proliferation, apoptosis, migration and invasion were determined by CCK-8, colony formation, flow cytometry, wound healing, and transwell assays, respectively. The bioinformatics prediction software RAID v2.0 was used to predict the putative binding sites. The interactions among HULC, miR-372-3p and HMGB1 were explored by luciferase assay and western blot assay. Results: Our results revealed elevated HULC and decreased miR-372-3p expression in both osteosarcoma tissues and cell lines. Overexpression of HULC or knockdown of miR-372-3p promoted osteosarcoma cell proliferation, migration and invasion and induced cell apoptosis. Bioinformatics and luciferase assays verified that HULC directly interacted with miR-372-3p to attenuate miR-372-3p binding to the HMGB1 3′-UTR. Furthermore, mechanistic investigations confirmed that activation of the miR-372-3p/HMGB1 regulatory loop by knockdown of miR-372-3p or overexpression of HMGB1 reversed the in vitro roles of HULC in promoting osteosarcoma cell proliferation, migration and invasion. Conclusion: Our study is the first to demonstrate that HULC may act as a ceRNA to modulate HMGB1 expression by competitively sponging miR-372-3p, leading to the regulation of osteosarcoma progression, which provides new insight into osteosarcoma diagnosis and treatment.

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“…Many miRNAs are deeply implicated at multiple steps of tumor diseases, including proliferation, invasion, adhesion, and metastasis . Currently, only a few studies have reported the function of miR‐541 in oncology.…”

Section: Discussionmentioning

confidence: 99%

“…Different types of cancers have different miRNA expression patterns . An increasing amount of evidence shows that miRNAs regulate various biological processes during tumorigenesis and metastasis . In addition, specific miRNAs inhibit carcinogenesis progression .…”

Section: Introductionmentioning

confidence: 99%

miR‐541 suppresses proliferation and invasion of squamous cell lung carcinoma cell lines via directly targeting high‐mobility group AT‐hook 2

et al. 2018

Cancer Medicine

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An increasing number of studies have demonstrated that micro‐ribonucleic acids (miRNAs) are important tumor suppressors during carcinogenesis. However, the function of miRNA‐541 (miR‐541) in malignancies, especially lung cancer, has not been widely reported. In this study, miR‐541 expression was significantly decreased in squamous cell lung carcinoma (SCLC) cancerous tissue and SCLC cell lines. To analyze miR‐541 function in SCLC, we overexpressed miR‐541 in SCLC cell lines (SK‐MES‐1 and H226). According to the CCK8, wound scratch, and transwell invasion assay results, miR‐541 overexpression significantly inhibited SCLC cell proliferation, migration, and invasion ability. Next, using RT‐PCR, Western blotting, immunocytochemistry, and luciferase assays, HMGA2 was identified, for the first time, as a direct regulatory target of miR‐541 in SK‐MES‐1 and H226 cells. Furthermore, upregulating HMGA2 expression significantly alleviated the suppressive effects of miR‐541 on SK‐MES‐1 and H226 cell proliferation, migration, and invasion. In summary, our study revealed that miR‐541 inhibited SCLC proliferation and invasion by directly targeting HMGA2.

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MiR‐372‐3p promotes cell growth and metastasis by targeting FGF9 in lung squamous cell carcinoma

Cited by 55 publications

References 40 publications

miR-30a-5p Inhibits Proliferation and Migration of Lung Squamous Cell Carcinoma Cells by Targeting FOXD1

miR-30a-5p Inhibits Proliferation and Migration of Lung Squamous Cell Carcinoma Cells by Targeting FOXD1

LncRNA HULC induces the progression of osteosarcoma by regulating the miR-372-3p/HMGB1 signalling axis

miR‐541 suppresses proliferation and invasion of squamous cell lung carcinoma cell lines via directly targeting high‐mobility group AT‐hook 2

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