MiR‐3691‐5p is upregulated in docosahexaenoic acid‐treated vascular endothelial cell and targets serpin family E member 1

Abstract

Endothelium, the inner cellular lining of blood vessels, has an important role in the regulation of physiological processes and its dysfunction may initiate cardiovascular complications. Previous investigations have revealed that dietary docosahexaenoic acid (DHA) is related to a lower possibility of cardiovascular disease and mortality. Until now, the molecular mechanisms in the biological activities of DHA remain largely unknown. MicroRNAs (miRNAs) play a vital role in regulating gene expression. Thus, we aimed to investigate whether DHA improves the dysfunction via regulating miRNAs. To understand the protective effects of DHA through modulating miR-3691-5p and its target genes for palmitic acid (PAL) induced apoptosis in endothelial cells. The present study demonstrated that DHA upregulated miR-3691-5p expression, and downregulated the expression of its target gene serpin family E member 1 (SERPINE1). MiR-mimics and inhibitors modulation results indicated that miR-3691-5p regulates endothelial apoptosis through activating antiapoptotic response which controlled by the STAT3 signaling pathway. In conclusion, we have shown that PAL-induced apoptosis could be decreased by DHA treatment through miR-3691-5/SERPINE1 pathways.

Inducible miR-1224 silences cerebrovascular Serpine1 and restores blood flow to the stroke-affected site of the brain

“… 62 In human umbilical vein endothelial cells, docosahexaenoic acid, which is known to be a healthy dietary factor, induced miR-3691-5p to post-transcriptionally silence SERPINE1. 63 In the setting of hepatopulmonary syndrome, PAI-1 in pulmonary microvascular endothelial cells is a direct target of miR-145-5p. 64 A separate study on human pulmonary microvascular endothelial cells reported on targeting of PAI-1 by miR-30c and miR-301a.…”