MiR-369-3p participates in endometrioid adenocarcinoma via the regulation of autophagy

Liu, Ping; Ma, Chengbin; Wu, Qing; Zhang, Wenying; Wang, Cao; Li, Yuan; Xi, Xiaowei

doi:10.1186/s12935-019-0897-8

Cited by 13 publications

(11 citation statements)

References 37 publications

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“…lncRNA is a competitive endogenous RNA with a miRNA recognition element, which can compete with mRNAs for the same miRNA response element, thus inhibiting the binding of miRNAs to mRNAs and affecting the regulatory effect of miRNAs on target genes ( 26 ). miR-369-3p expression has been found to be decreased in endometrioid adenocarcinoma tissues and miR-369-3p overexpression suppresses cell proliferation and migration in endometrioid adenocarcinoma ( 27 ). miR-369-3p expression has been shown to be notably increased in Hirschsprung's disease and the dysregulation of miR-369-3p has been shown to decrease the proliferation and migration of the SH-SY5Y and 293T cells ( 28 ).…”

Section: Discussionmentioning

confidence: 99%

Knockdown of long non‑coding RNA DLEU2 suppresses idiopathic pulmonary fibrosis by regulating the microRNA‑369‑3p/TRIM2 axis

Luo

Xiao

et al. 2021

Int J Mol Med

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Idiopathic pulmonary fibrosis (IPF) is the most common form of idiopathic interstitial pneumonia with an increasing incidence. In the present study, Genome Expression Omnibus (GEO) datasets (GSE10667, GSE24206 and GSE32537) were applied to identify lncRNA DLEU2 in IPF. Through prediction using starBase, TargetScan, miRTarBase and miRDB, tripartite motif containing 2 (TRIM2) and prostaglandin F2 receptor inhibitor (PTGFRN) were found to be upregulated in IPF. DLEU2 expression, the mRNA expression of TRIM2 and PTGFRN, and miR-369-3p expression in A549 cells and lung tissues were detected by RT-qPCR. The protein expression of TRIM2 and PTGFRN in lung tissues and A549 cells was detected by western blot analysis. The proliferation and migration of A549 cells was respectively detected by CCK-8 assay and wound healing assay. The expression of collagen I, α-smooth muscle actin (SMA) and E-cadherin was detected by immunofluorescence assay in A549 cells, and collagen I expression was detected by immunohistochemistry assay in lung tissues. The expression of collagen I, α-SMA and E-cadherin was also detected by western blot analysis in A549 cells and lung tissues. Dual-luciferase reporter assay was used to confirm the association between DLEU2 and miR-369-3p, and miR-369-3p and TRIM2. As a result, DLEU2 expression was found to be upregulated in IPF and in transforming growth factor (TGF)-β1-stimulated A549 cells. The silencing of DLEU2 inhibited the TGF-β1-induced proliferation, migration and epithelial-mesenchymal transition (EMT) of A549 cells and bleomycin (BLM)-induced pulmonary fibrosis in mice. TRIM2 expression was increased and miR-369-3p expression was decreased in the lung tissues of mice with BLM-induced fibrosis and in TGF-β1-stimulated A549 cells. DLEU2 directly targeted miR-369-3p. The effect of the silencing of DLEU2 on TGF-β1-stimulated A549 cells was suppressed by the silencing of miR-369-3p. TRIM2 was the target protein of miR-369-3p. On the whole, the present study demonstrates that the silencing of DLEU2 suppressed IPF by upregulating miR-369-3p expression and downregulating TRIM2 expression.

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Section: Discussionmentioning

confidence: 99%

Knockdown of long non‑coding RNA DLEU2 suppresses idiopathic pulmonary fibrosis by regulating the microRNA‑369‑3p/TRIM2 axis

Luo

Xiao

et al. 2021

Int J Mol Med

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“…In colorectal cancer, increased ATG10 expression is associated with lymph node metastasis and lymphovascular invasion [ 58 ]. ATG10 is a target gene of miR-369-3p, which inhibits cell proliferation and migration by targeting cancer cells via autophagy in endometrioid adenocarcinoma [ 59 ].…”

Section: Discussionmentioning

confidence: 99%

Genomic variations and signatures of selection in Wuhua yellow chicken

Weng

et al. 2020

PLoS ONE

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Wuhua yellow chicken (WHYC) is an important traditional yellow-feathered chicken from China, which is characterized by its white tail feathers, white flight feathers, and strong disease resistance. However, the genomic basis of these unique traits associated with WHYC is poorly understood. In this study, whole-genome resequencing was performed with an average coverage of 20.77-fold to investigate heritable variation and identify selection signals in WHYC. Reads were mapped onto the chicken reference genome (Galgal5) with a coverage of 85.95%. After quality control, 11,953,471 single nucleotide polymorphisms and 1,069,574 insertion/deletions were obtained. In addition, 41,408 structural variants and 33,278 copy number variants were found. Comparative genomic analysis of WHYC and other yellow-feathered chicken breeds showed that selected regions were enriched in genes involved in transport and catabolism, immune system, infectious diseases, signal transduction, and signaling molecules and interactions. Several genes associated with disease resistance were also identified, including IFNA , IFNB , CD86 , IL18 , IL11RA , VEGFC , and ATG10 . Furthermore, our results suggest that PMEL and TYRP1 may contribute to the white feather coloring in WHYC. These findings can improve our understanding of the genetic characteristics of WHYC and may contribute to future breed improvement.

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“…Liu P. et al have reported that miR-369-3p, similarly to miR-365, can also act as a tumor suppressor, inducing apoptosis and inhibiting cell migration by targeting ATG10 in endometrioid adenocarcinoma [ 110 ]. On the contrary, miR-200, miR-296-5p and miR-654 have been described in different studies as oncomirs [ 86 , 111 , 112 , 113 , 114 ].…”

Section: Discussionmentioning

confidence: 99%

Analysis of a Preliminary microRNA Expression Signature in a Human Telangiectatic Osteogenic Sarcoma Cancer Cell Line

Palmini

Romagnoli

Donati

et al. 2021

IJMS

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Telangiectatic osteosarcoma (TOS) is an aggressive variant of osteosarcoma (OS) with distinctive radiographic, gross, microscopic features, and prognostic implications. Despite several studies on OS, we are still far from understanding the molecular mechanisms of TOS. In recent years, many studies have demonstrated not only that microRNAs (miRNAs) are involved in OS tumorigenesis, development, and metastasis, but also that the presence in high-grade types of OS of cancer stem cells (CSCs) plays an important role in tumor progression. Despite these findings, nothing has been described previously about the expression of miRNAs and the presence of CSCs in human TOS. Therefore, we have isolated/characterized a putative CSC cell line from human TOS (TOS-CSCs) and evaluated the expression levels of several miRNAs in TOS-CSCs using real-time quantitative assays. We show, for the first time, the existence of CSCs in human TOS, highlighting the in vitro establishment of this unique stabilized cell line and an identification of a preliminary expression of the miRNA profile, characteristic of TOS-CSCs. These findings represent an important step in the study of the biology of one of the most aggressive variants of OS and the role of miRNAs in TOS-CSC behavior.

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MiR-369-3p participates in endometrioid adenocarcinoma via the regulation of autophagy

Cited by 13 publications

References 37 publications

Knockdown of long non‑coding RNA DLEU2 suppresses idiopathic pulmonary fibrosis by regulating the microRNA‑369‑3p/TRIM2 axis

Knockdown of long non‑coding RNA DLEU2 suppresses idiopathic pulmonary fibrosis by regulating the microRNA‑369‑3p/TRIM2 axis

Genomic variations and signatures of selection in Wuhua yellow chicken

Analysis of a Preliminary microRNA Expression Signature in a Human Telangiectatic Osteogenic Sarcoma Cancer Cell Line

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