miR-3587 Targets HMOX1 to Attenuate Ferroptotic Renal Injury Following Ischemia-Reperfusion

Abstract

Renal ischemia-reperfusion (IR) is frequently observed in patients who are critically ill, yet there are no reliable or effective approaches to treating this condition. This study aimed to investigate the miRNA-mRNA regulatory networks that are involved in IR-related ferroptotic renal injury. Bioinformatics method was used to screen critical hub gene and its upstream miRNA from Renal IR-related data sets in Gene Expression Omnibus, and further experiments were conducted to verify the regulatory effect of miRNA on critical hub gene. Heme oxygenase-1 (HMOX1) was identified as a critical hub gene that was found to be significantly upregulated during the early stages of renal IR injury. miR-3587 was identified as a putative regulator of HMOX1. When a miR-3587 inhibitor was applied in a hypoxia-reoxygenation model system using renal tubular epithelial cells, HMOX1 expression was significantly increased relative to that observed in the control hypoxia-reoxygenation group, with concomitant increases in glutathione peroxidase 4 (GPX4) protein levels, enhanced cell viability, a reduction in malondialdehyde content, and the restoration of normal mitochondrial membrane potential. Preliminary evidence suggests that utilizing miR-3587 inhibitors can further promote HMOX1 upregulation, thereby protecting renal tissues from IR-induced ferroptosis.