MiR-351 transiently increases during muscle regeneration and promotes progenitor cell proliferation and survival upon differentiation

Abstract

Chen Y, Melton DW, Gelfond JAL, McManus LM, Shireman PK. MiR-351 transiently increases during muscle regeneration and promotes progenitor cell proliferation and survival upon differentiation. Physiol Genomics 44: 1042-1051, 2012. First published September 11, 2012; doi:10.1152/physiolgenomics.00052.2012.-MicroRNAs (miRNAs) regulate many biological processes including muscle development. However, little is known regarding miRNA regulation of muscle regeneration. Murine tibialis anterior muscle was evaluated after cardiotoxin-induced injury and used for global miRNA expression analysis. From day 1 through day 21 following injury, 298 miRNAs were significantly changed at least at one time point, including 86 miRNAs that were altered Ͼ10-fold compared with uninjured skeletal muscle. Temporal miRNA expression patterns included inflammation-related miRNAs (miR-223 and -147) that increased immediately after injury; this pattern contrasted to that of mature muscle-specific miRNAs (miR-1, -133a, and -499) that abruptly decreased following injury followed by upregulation in later regenerative events. Another cluster of miRNAs were transiently increased in the early days of muscle regeneration including miR-351, a miRNA that was also transiently expressed during myogenic progenitor cell (MPC) differentiation in vitro. Based on computational predictions, further studies demonstrated that E2f3 was a target of miR-351 in myoblasts. Moreover, knockdown of miR-351 expression inhibited MPC proliferation and promoted apoptosis during MPC differentiation, whereas miR-351 overexpression protected MPC from apoptosis during differentiation. Collectively, these observations suggest that miR-351 is involved in both the maintenance of MPC proliferation and the transition into differentiated myotubes. Thus, a novel, timedependent sequence of molecular events during muscle regeneration has been identified; miR-351 inhibits E2f3 expression, a key regulator of cell cycle progression and proliferation, and promotes MPC proliferation and protects early differentiating MPC from apoptosis, important events in the hostile tissue environment after acute muscle injury.microRNA; myogenic progenitor cell; muscle regeneration; muscle differentiation SKELETAL MUSCLES ARE DAMAGED and repaired repeatedly throughout life. The capacity for skeletal muscle growth and regeneration is conferred by satellite cells located between the basal lamina and the sarcolemma of mature myofibers (21). Upon injury, satellite cells reenter the cell cycle, proliferate, and then exit the cell cycle either to renew the quiescent satellite cell pool or to differentiate into mature myofibers (1). Despite recent advances, genes involved in these processes are still largely unknown. Understanding the molecular mechanisms that regulate satellite cell activities could promote development of novel countermeasures to enhance muscle regeneration that is compromised by diseases or aging.Both cell proliferation and differentiation programs are essential for muscle regeneration. The dec...

MicroRNA-351 inhibits denervation-induced muscle atrophy by targeting TRAF6

“…A previous study observed that miR-351 is transiently increased in early days of muscle regeneration (26). miR-351 inhibits the expression of E2f3, a key regulator of cell cycle progression and proliferation, promotes myogenic progenitor cell proliferation and protects early differentiating myogenic progenitor cell from apoptosis (26).…”

Long noncoding RNAs and their roles in skeletal muscle fate determination

“…The G0 phase occurs directly before, or concurrently with differentiation. To summarize the process of SC fate specification: Pax3 and Pax7 function to maintain the undifferentiated SC pool, MyoD and Myf5 function as primary myogenic regulatory factors (MRF) to promote myoblast differentiation, and myogenin and MRF4 act as secondary MRFs in terminal cell differentiation (12). …”

Uvragtargeting byMir125aandMir351modulates autophagy associated withEwsr1deficiency

“…38 In addition, Mir351 targets TMEM59 (transmembrane protein 59) 39 and E2f3 (E2F transcription factor 3). 40 Even though Wang et al 41 have proposed that Uvrag might be a potential target gene for Mir351, no study has proven or validated that possibility. Our data show that Uvrag is a direct target of both Mir125a and Mir351 and that EWSR1 deficiency downregulates UVRAG via a miRNA-dependent pathway at the post-transcriptional level.…”