mir-35 is involved in intestine cell G1/S transition and germ cell proliferation in C. elegans

Liu, Min; Liu, Pengpeng; Zhang, Li; Cai, Qingchun; Gao, Ge; Zhang, Wenxia; Zhu, Zuoyan; Dong, Li; Fan, Qing

doi:10.1038/cr.2011.102

Cited by 35 publications

(40 citation statements)

References 69 publications

(74 reference statements)

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“…Alternatively, LST-1 may promote the activity of another direct repressor of GLD-1 levels. Our data do not support that the mir-35 family are repressors of GLD-1 accumulation, as previously proposed (Liu et al 2011), and other direct repressors of GLD-1 levels remain to be identified. Total no.…”

Section: Gld-1 Levels In Germ Cells Irreversibly Committed To Meioticcontrasting

confidence: 51%

“…fbf-1(ok224); mir-35 fam(0); glp-1(bn18) failed to show premature meiotic entry defects consistent with failure to repress GLD-1 ( Figure S5B) and GLD-1 levels were not further elevated in fbf-1(ok91) mir-35 fam(0); glp-1(bn18) compared to fbf-1(ok224); glp-1(bn18) ( Figure S5C). Therefore, we think it unlikely that the mir-35 family acts to repress GLD-1 in germline stem cells, as previously proposed (Liu et al 2011). Instead, the mir-35 family may promote a large proliferative zone reminiscent of genes that maintain normal physiology (i.e., eat-2).…”

Section: Gld-1 Levels Are Insensitive To Gld-1 Gene Dosementioning

confidence: 83%

“…The mir-35 family of microRNAs consists of eight microRNAs (mir-35 through mir-42) that were proposed to negatively regulate gld-1 to promote proliferative zone size (Liu et al 2011), however GLD-1 levels were not examined. mir-35-41(nDf50) mir-42-44(nDf49) mutants [mir-35 fam(0)] had smaller proliferative zone sizes compared to wild type (Table 1), consistent with a potential role in repressing GLD-1 levels.…”

Section: Gld-1 Levels Are Insensitive To Gld-1 Gene Dosementioning

confidence: 99%

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Germline Stem Cell Differentiation Entails Regional Control of Cell Fate Regulator GLD-1 in Caenorhabditis elegans

2016

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Germline stem cell differentiation in Caenorhabditis elegans is controlled by glp-1 Notch signaling. Cell fate regulator GLD-1 is sufficient to induce meiotic entry and expressed at a high level during meiotic prophase, inhibiting mitotic gene activity. glp-1 signaling and other regulators control GLD-1 levels post-transcriptionally (low in stem cells, high in meiotic prophase), but many aspects of GLD-1 regulation are uncharacterized, including the link between glp-1-mediated transcriptional control and post-transcriptional GLD-1 regulation. We established a sensitive assay to quantify GLD-1 levels across an 35-cell diameter field, where distal germline stem cells differentiate proximally into meiotic prophase cells in the adult C. elegans hermaphrodite, and applied the approach to mutants in known or proposed GLD-1 regulators. In wild-type GLD-1 levels elevated 20-fold in a sigmoidal pattern. We found that two direct transcriptional targets of glp-1 signaling, lst-1 and sygl-1, were individually required for repression of GLD-1. We determined that lst-1 and sygl-1 act in the same genetic pathway as known GLD-1 translational repressor fbf-1, while lst-1 also acts in parallel to fbf-1, linking glp-1-mediated transcriptional control and post-transcriptional GLD-1 repression. Additionally, we estimated the position in wild-type gonads where germ cells irreversibly commit to meiotic development based on GLD-1 levels in worms where glp-1 activity was manipulated to cause an irreversible fate switch. Analysis of known repressors and activators, as well as modeling the sigmoidal accumulation pattern, indicated that regulation of GLD-1 levels is largely regional, which we integrated with the current view of germline stem cell differentiation.

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Section: Gld-1 Levels In Germ Cells Irreversibly Committed To Meioticcontrasting

confidence: 51%

Section: Gld-1 Levels Are Insensitive To Gld-1 Gene Dosementioning

confidence: 83%

Section: Gld-1 Levels Are Insensitive To Gld-1 Gene Dosementioning

confidence: 99%

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Germline Stem Cell Differentiation Entails Regional Control of Cell Fate Regulator GLD-1 in Caenorhabditis elegans

2016

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“…Previously, Alvarez-Saavedra and colleagues comprehensively analyzed the function of the miR-35 family members in C. elegans , and found that mutation of seven members of this family led to developmental suppression, including embryonic and larval lethality [47]. More recently, this family was found to regulate the G1/S transition of intestinal cells and promote proliferation of germ cells in C. elegans [48]. Further, in the parasitic nematode of Ascaris suum , each asu-miR-36 family member was expressed in a narrow window from early to middle embryogenesis, implying that each member from this family may finely regulate the development of the parasite [41].…”

Section: Resultsmentioning

confidence: 99%

A Deep Analysis of the Small Non-Coding RNA Population in Schistosoma japonicum Eggs

et al. 2013

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Background Schistosoma japonicum is a parasitic flatworm that causes zoonotic schistosomiasis. The typical outcome of schistosomiasis is hepatic granuloma and fibrosis, which is primarily induced by soluble egg-derived antigens. Although schistosomal eggs represent an important pathogenic stage to the host, the biology of this critical stage is largely unknown. We previously investigated the expression profiles of sncRNAs during different developmental stages of this parasite. However, using small RNA extracted from egg-deposited liver tissues generated limited information about sncRNAs in eggs. Here, we characterized the complete small RNAome in this stage of the parasite after optimization of RNA purification.Methodology and Principal FindingsA library, SjE, was constructed with the small RNA extracted from S. japonicum eggs and subjected to high-throughput sequencing. The data were depicted by comprehensive bioinformatic analysis to explore the expression features of sncRNAs in the egg stage. MicroRNAs accounted for about one quarter of the total small RNA population in this stage, with a strongly biased expression pattern of certain miRNA family members. Sja-miR-71, sja-miR-71-5p, and sja-miR-36-3p were suggested to play important roles in embryo development. A panel of transfer RNA fragments (tRFs) precisely processed from the 5′ end of mature tRNAs was identified for the first time, which represented a strong egg stage-biased expression. The tRNA-Ala derived small RNAs were the most highly expressed Sj-tRFs in eggs. Further, the expression of siRNAs from 29 types of well-defined transposable elements (TEs) was observed to be relatively stable among different developmental stages.Conclusions and SignificanceIn this study, we characterized the sncRNA profile in the egg stage of S. japonicum. Featured expression of sncRNAs, especially the tRNA-derived small RNAs, was identified, which was further compared with that of other developmental stages. These novel findings would facilitate a deeper understanding of the biology of schistosomal parasites.

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“…While the RNA-binding proteins GLD-1, GLD-2/GLD-3 promote the meiosis entry of germline stem cells, the activation of the GLP-1/Notch signaling pathway inhibits these signals and retains the cells in mitotic stage [47]. Interestingly, gld-1 mRNA has been found in ALG-1-immunoprecipitated complex [48], and other evidence also indicates that gld-1 is subject to regulation by miRNAs [49]. These studies suggest the possibility that miRNAs can regulate the mitosis to meiosis decision by controlling key genes involved in the process.…”

Section: Discussionmentioning

confidence: 99%

The microRNA pathway controls germ cell proliferation and differentiation in C. elegans

et al. 2012

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The discovery of the miRNA pathway revealed a new layer of molecular control of biological processes. To uncover new functions of this gene regulatory pathway, we undertook the characterization of the two miRNA-specific Argonaute proteins in Caenorhabditis elegans, ALG-1 and ALG-2. We first observed that the loss-of-function of alg-1 and alg-2 genes resulted in reduced progeny number. An extensive analysis of the germline of these mutants revealed a reduced mitotic region, indicating fewer proliferating germ cells. We also observed an early entry into meiosis in alg-1 and alg-2 mutant animals. We detected ALG-1 and ALG-2 protein expressions in the distal tip cell (DTC), a specialized cell located at the tip of both C. elegans gonadal arms that regulates mitosis-meiosis transition. Re-establishing the expression of alg-1 specifically in the DTC of mutant animals partially rescued the observed germline defects. Further analyses also support the implication of the miRNA pathway in gametogenesis. Interestingly, we observed that disruption of five miRNAs expressed in the DTC led to similar phenotypes. Finally, gene expression analysis of alg-1 mutant gonads suggests that the miRNA pathway is involved in the regulation of different pathways important for germline proliferation and differentiation. Collectively, our data indicate that the miRNA pathway plays a crucial role in the control of germ cell biogenesis in C. elegans.

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mir-35 is involved in intestine cell G1/S transition and germ cell proliferation in C. elegans

Cited by 35 publications

References 69 publications

Germline Stem Cell Differentiation Entails Regional Control of Cell Fate Regulator GLD-1 in Caenorhabditis elegans

Germline Stem Cell Differentiation Entails Regional Control of Cell Fate Regulator GLD-1 in Caenorhabditis elegans

A Deep Analysis of the Small Non-Coding RNA Population in Schistosoma japonicum Eggs

The microRNA pathway controls germ cell proliferation and differentiation in C. elegans

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