miR-34a inhibits the migration and invasion of esophageal squamous cell carcinoma by targeting Yin Yang-1

Nie, Jing; Ge, Xin; Geng, Yangyang; Cao, Han; Zhu, Wei; Jiao, Yang; Wu, Jinchang; Zhou, Jundong; Cao, Jianping

doi:10.3892/or.2015.3962

Cited by 45 publications

(30 citation statements)

References 33 publications

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“…Deregulated miRNAs have an important role in many malignancies which have recently been identified as oncomir or tumor suppressor in many cancers [21][22][23]. ESCC is one of these malignancies in which altered miRNAs expression has been reported [24,52,53].…”

Section: Discussionmentioning

confidence: 98%

“…A number of studies have reported the potential clinical roles of miRNAs in several diseases such as breast cancer [15], cervical cancer [16], colorectal cancer [17], gastric cancer [18], bladder cancer [19], lung cancers [20], etc., in which altered expression patterns of miRNAs have been shown to act in the oncogenic or tumor suppressor pathways [21][22][23]. Furthermore, altered miRNA expression has been reported in esophageal cancers [24][25][26].…”

Section: Introductionmentioning

confidence: 98%

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Deregulation of miR-93 and miR-143 in human esophageal cancer

et al. 2015

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Esophageal squamous cell carcinoma (ESCC) is the second and third most common malignancy in Iranian males and females, respectively. Treatment of ESCC is largely ineffective due to lack of detection at early stages of the disease. In recent years, miRNA, a small RNA molecule, has drawn much attention to researchers as a potential biomarker for esophageal cancer. miR-93 and miR-143 are two miRNA molecules reported to be frequently deregulated in various cancers, including prostate, stomach, cervix, and etc. The purpose of this study was to investigate the expression levels of these miRNAs and evaluate their diagnostic and therapeutic potential in esophageal squamous cell carcinoma. In this study, total RNA was extracted from 30 tumor tissues and 30 nontumor tissues of esophageal tumor margins, using RNX-plus solution. After validating the quality and quantity of total RNA, cDNAs of interest were synthesized using microRNA-specific cDNA Synthesis Kit. The expression level of miR-93 and miR-143 was evaluated using quantitative real-time PCR with miRNA-specific primers. Finally, the obtained data was analyzed by SPSS ver.20 software and paired t test was performed to observe the significance of difference between groups. The expression level of miR-93 was significantly increased and of miR-143 was significantly decreased in most of the examined tumor tissues, compared to nontumor tissues. Also, our findings did not detect correlation between mir-93 and mir-143 expressions in regard to stage and grade of the samples. These findings suggest that the deregulation of these miRNAs may play an important role in esophageal squamous cell carcinoma. Both miR-93 and miR-143 might be used as potential biomarkers in esophageal squamous cell carcinoma. However, more studies with large population of samples are necessary.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 98%

Deregulation of miR-93 and miR-143 in human esophageal cancer

et al. 2015

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“…Wild type p53 induces the transcription of miR-34a which targets several molecules that are involved in cellular transformation and carcinogenesis [27–29]. The abnormal miR-34a expression has been revealed in breast cancer [31], colon cancer [32], cervical cancer [33], prostate cancer [34], esophageal squamous cell carcinoma [37], and lung cancer [38]. MiR-34a may act on its target genes to regulate the proliferation, apoptosis, invasion, metastasis and epithelial mesenchymal transition of cancer cells, exert inhibitory effects on the growth and metastasis of cancers [39].…”

Section: Discussionmentioning

confidence: 99%

MiR-34a and miR-206 act as novel prognostic and therapy biomarkers in cervical cancer

et al. 2017

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BackgroundRecent evidence indicated that the aberrant expression of microRNA plays a crucial role in the development of cervical cancer. The overall shorter survival was strongly related to the abnormal expression of microRNA-34a (miR-34a) and microRNA-206 (miR-206), which target B cell lymphoma-2(Bcl2) and c-Met. Hepatocyte growth factor (HGF)/c-Met pathway is related to the occurrence, development and prognosis of cervical cancer, and c-Met is significantly overexpressed in cervical squamous cell carcinoma. Bcl2 is also considered to be a promising target for developing novel anticancer treatments.MethodsIn this study, we detect the expression of miR-34a and miR-206 in the cervical cancer tissue through quantificational real-time polymerase chain reaction (qRT-PCR) assay, and the expression of Bcl2 and c-Met from cervical cancer tissue were detected by immunohistochemistry.ResultsThe expression of miR-34a and miR-206 were down-regulated in the cervical cancer tissue through qRT-PCR assay. As target genes of miR-34a and miR-206, Bcl2 and c-Met were up-regulated in cervical cancer tissues through qRT-PCR assay and immunohistochemistry. Kaplan–Meier and log-rank analysis revealed that down-regulated expression of miR-34a and miR-206 were strongly related to shorter overall survival. Multivariate Cox proportional hazards model for all variables that were statistically significant in the univariate analysis demonstrated that miR-34a (P = 0.038) and miR-206 (P = 0.008) might be independent prognostic factors for overall survival of patients suffering from cervical cancer.ConclusionsThe up-regulation of Bcl2 and c-Met promotes the cervical cancer’s progress, and the expression of miR-34a and miR-206 significantly correlated with the progression and prognosis in cervical cancer. All of these suggested that miR-34a and miR-206 might be the novel prognostic and therapy tools in cervical cancer.Electronic supplementary materialThe online version of this article (doi:10.1186/s12935-017-0431-9) contains supplementary material, which is available to authorized users.

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“…The downregulated expression of miR-34a-controlled genes may occur due to mutations that inactivate p53 in tumor cells (Balca-Silva et al, 2012). Recently, Nie et al (2015) found that miR-34a was downregulated in ESCC tissues compared with that in normal tissues (P < 0.05), and that miR-34a overexpression increased apoptosis and decreased clonogenic formation, whereas it inhibited invasion and migration of ESCC cells by suppressing MMP-2 and -9 expression. However, the clinical significance and prognostic value of miR-34a in ESCC had not yet been investigated.…”

Section: Introductionmentioning

confidence: 99%

Clinical significance of microRNA-34a in esophageal squamous cell carcinoma

Lin

Chen

et al. 2015

Genet. Mol. Res.

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ABSTRACT. has been found to be downregulated in esophageal squamous cell carcinoma (ESCC) tissues compared with that in normal tissues (P < 0.05), and miR-34a overexpression increased apoptosis and decreased clonogenic formation. However, the clinical significance and prognostic value of miR-34a in ESCC has not yet been investigated. In total, 111 patients with ESCC diagnosed and treated at the Department of Thoracic Surgery of Fujian Provincial Hospital between March 2008 and February 2014 were included in this retrospective study. Quantitative real-time PCR was performed to detect expression levels of miR-34a. The associations between miR-34a expression and clinicopathological features were analyzed using χ 2 tests. For analysis of survival data, Kaplan-Meier curves were constructed, and the log-rank test was performed. The expression levels of miR-34a in ESCC tissues were significantly decreased (P < 0.01), compared with those in adjacent normal esophageal tissues. Low miR-34a expression in ESCC tissues was significantly associated with tumor differentiation (P = 0.013), lymph node status (P = 0.038), and advanced clinical stage (P < 0.001). The KaplanMeier analysis and log-rank test revealed that low miR-34a levels had a 17685 MicroRNA-34a in esophageal cancer ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 17684-17691 (2015) significant impact on overall survival of patients with ESCC (P = 0.006). Multivariate analyses showed that the expression of miR-34a was an independent prognostic factor for ESCC (P = 0.018). Our findings indicate that there is reduced expression of miR-34a in human ESCC tissues and suggest a crucial role for miR-34a downregulation in ESCC progression and prognosis.

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miR-34a inhibits the migration and invasion of esophageal squamous cell carcinoma by targeting Yin Yang-1

Cited by 45 publications

References 33 publications

Deregulation of miR-93 and miR-143 in human esophageal cancer

Deregulation of miR-93 and miR-143 in human esophageal cancer

MiR-34a and miR-206 act as novel prognostic and therapy biomarkers in cervical cancer

Clinical significance of microRNA-34a in esophageal squamous cell carcinoma

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