miR-340 alleviates chemoresistance of osteosarcoma cells by targeting ZEB1

Yan, Haibin; Zhang, Bingyun; Fang, Chongbin; Chen, Liqiu

doi:10.1097/cad.0000000000000614

Cited by 26 publications

(18 citation statements)

References 15 publications

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“…This finding suggests that ZEB1 takes part in the miR-708-5p-mediated suppress of the migration and invasion of OS cells. ZEB1 is highly expressed in OS tissues when compared to normal tissue (18,40). Moreover, the protein level of ZEB1 was found to be upregulated in OS MG63 and SaOS-2 cells unlike that in the hMSCs and HS-5 cells.…”

Section: Discussionmentioning

confidence: 90%

The microRNA‑708‑5p/ZEB1/EMT axis mediates the metastatic potential of osteosarcoma

et al. 2019

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MicroRNA-708-5p (miR-708-5p) and epithelialto-mesenchymal transition (EMT) have been widely identified to contribute to the pathogenesis and progression of multiple cancers. However, the connection between miR-708-5p and EMT has not been sufficiently clarified. Therefore, our research aimed to investigate the impact of miR-708-5p on EMT and the metastasis of osteosarcoma (OS). We first analyzed the differentially expressed microRNAs (DEmiRNAs) from the GSE70367 dataset. We found that the expression of miR-708-5p was lower in OS cells. Overexpression of miR-708-5p was able to impair the migration and invasion of OS cells. Moreover, miR-708-5p inhibited EMT of OS cells MG63 and SaOS-2, wherein E-cadherin was increased, and N-cadherin, vimentin, and Snail were decreased. Semaphorin 4C (SEMA4C), mitogen-activated protein kinase kinase kinase 3 (MAP3K3), and zinc finger E-box-binding homeobox 1 (ZEB1) were predicted as target genes of miR-708-5p by bioinformatics method. Only ZEB1, one of the EMT-inducing transcription factors, was validated as the direct target gene of miR-708-5p in OS cells through dual-luciferase reporter assay and Western blot analysis. Knockdown of ZEB1 was found to inhibit the metastasis of MG63 and SaOS-2 cells, whereas ZEB1 overexpression promoted their metastasis. In summary, miR-708-5p impaired the metastasis and EMT of OS, which was found to be mediated by inhibition of ZEB1.

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Section: Discussionmentioning

confidence: 90%

The microRNA‑708‑5p/ZEB1/EMT axis mediates the metastatic potential of osteosarcoma

et al. 2019

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“…MIAT promotes ZEB1 expression in osteosarcoma. ZEB1 is important for the proliferation and invasion of osteosarcoma cells (19). Thus, it was investigated whether MIAT affects ZEB1 expression in osteosarcoma cells.…”

Section: Resultsmentioning

confidence: 99%

Long non‑coding RNA MIAT competitively binds miR‑150‑5p to regulate ZEB1 expression in osteosarcoma

et al. 2018

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Long non-coding RNAs (LncRNAs), are significant in a number of biological stages and illnesses. The myocardial infarction associated transcript (MIAT) serves a function in numerous types of illness and physiological and pathological processes, including paranoid schizophrenia, diabetic retinopathy, myocardial infarction and neuroendocrine prostate cancer. However, the function of the lncRNA MIAT in the development of osteosarcoma is unknown. It has been identified that during the development of osteosarcoma, MIAT is upregulated in tumor tissues compared to adjacent non-tumor tissues. The spreading and proliferation of osteosarcoma cells was reduced by MIAT knockdown. These findings indicate that MIAT functions by competing with critical RNAs to target miR-150-5p and activate zinc finger E-box binding homeobox 1 to modulate the function of osteosarcoma cells. Together, the present findings may contribute to the understanding of the pathogenesis of osteosarcoma.

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“…[18][19][20] The administration of synthetic miR-590-3p lipid formulations immediately after MI in mice results in marked reduction of infarct size and persistent recovery of cardiac function. 18 Besides, we previously predicted that zinc finger E-box binding homeobox 1 (ZEB1) gene, which is a transcription factor and is known for the proliferation and invasion of various cells 21,22 including human cardiomyocytes, 10 is the potential target of miR-590-3p.…”

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confidence: 99%

MiR‐590‐3p regulates proliferation, migration and collagen synthesis of cardiac fibroblast by targeting ZEB1

Yuan

Pan

Wen

et al. 2019

J Cellular Molecular Medi

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Previous studies have implicated the attractive and promising role of miR‐590‐3p to restore the cardiac function following myocardial infarction (MI). However, the molecular mechanisms for how miR‐590‐3p involves in cardiac fibrosis remain largely unexplored. Using human cardiac fibroblasts (HCFs) as the cellular model, luciferase report assay, mutation, EdU assay and transwell migration assay were applied to investigate the biological effects of miR‐590‐3p on the proliferation, differentiation, migration and collagen synthesis of cardiac fibroblasts. We found that miR‐590‐3p significantly suppressed cell proliferation and migration of HCFs. The mRNA and protein expression levels of α‐SMA, Col1A1 and Col3A were significantly decreased by miR‐590‐3p. Moreover, miR‐590‐3p directly targeted at the 3’UTR of ZEB1 to repress the translation of ZEB1. Interfering with the expression of ZEB1 significantly decreased the cell proliferation, migration activity, mRNA and protein expressions of α‐SMA, Col1A1 and Col3A. Furthermore, the expressions of miR‐590‐3p and ZEB1 were identified in infarct area of MI model in pigs. Collectively, miR‐590‐3p suppresses the cell proliferation, differentiation, migration and collagen synthesis of cardiac fibroblasts by targeting ZEB1. These works will provide useful biological information for future studies on potential roles of miR‐590‐3p as the therapeutic target to recover cardiac function following MI.

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miR-340 alleviates chemoresistance of osteosarcoma cells by targeting ZEB1

Cited by 26 publications

References 15 publications

The microRNA‑708‑5p/ZEB1/EMT axis mediates the metastatic potential of osteosarcoma

The microRNA‑708‑5p/ZEB1/EMT axis mediates the metastatic potential of osteosarcoma

Long non‑coding RNA MIAT competitively binds miR‑150‑5p to regulate ZEB1 expression in osteosarcoma

MiR‐590‐3p regulates proliferation, migration and collagen synthesis of cardiac fibroblast by targeting ZEB1

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