MiR-328 suppresses the survival of esophageal cancer cells by targeting PLCE1

Han, Ning; Zhao, Wenchao; Zhang, Zhongmian; Zheng, Pengyuan

doi:10.1016/j.bbrc.2016.01.020

Cited by 43 publications

(31 citation statements)

References 31 publications

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“…Moreover, miR-328, which interacts with PDLIM5, has Cellular Physiology and Biochemistry Cellular Physiology and Biochemistry been found downregulated in multiple cancers. miR-328 inhibits the survival of cancer cells and enhances the sensitivity of cancer to radiotherapy [29,30]. Furthermore, overexpression of miR-328 reversed drug resistance and inhibited cell invasion of cancer stem cell-like SP cells in colorectal cancer [31].…”

Section: Discussionmentioning

confidence: 99%

Whole-Transcriptome Analysis of CD133+CD144+ Cancer Stem Cells Derived from Human Laryngeal Squamous Cell Carcinoma Cells

Zhang²,

Niu³

et al. 2018

Cell Physiol Biochem

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Background/Aims: CD133+CD44+ cancer stem cells previously isolated from laryngeal squamous cell carcinoma (LSCC) cell lines showed strong malignancy and tumorigenicity. However, the molecular mechanism underlying the enhanced malignancy remained unclear. Methods: Cell proliferation assay, spheroid-formation experiment, RNA sequencing (RNA-seq), miRNA-seq, bioinformatic analysis, quantitative real-time PCR, migration assay, invasion assay, and luciferase reporter assay were used to identify differentially expressed mRNAs, lncRNAs, circRNAs and miRNAs, construct transcription regulatory network, and investigate functional roles and mechanism of circRNA in CD133+CD44+ laryngeal cancer stem cells. Results: Differentially expressed genes in TDP cells were mainly enriched in the biological processes of cell differentiation, regulation of autophagy, negative regulation of cell death, regulation of cell growth, response to hypoxia, telomere maintenance, cellular response to gamma radiation, and regulation of apoptotic signaling, which are closely related to the malignant features of tumor cells. We constructed the regulatory network of differentially expressed circRNAs, miRNAs and mRNAs. qPCR findings for the expression of key genes in the network were consistent with the sequencing data. Moreover, our data revealed that circRNA hg19_circ_0005033 promotes proliferation, migration, invasion, and chemotherapy resistance of laryngeal cancer stem cells. Conclusions: This study provides potential biomarkers and targets for LSCC diagnosis and therapy, and provide important evidences for the heterogeneity of LSCC cells at the transcription level.

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Section: Discussionmentioning

confidence: 99%

Whole-Transcriptome Analysis of CD133+CD144+ Cancer Stem Cells Derived from Human Laryngeal Squamous Cell Carcinoma Cells

Zhang²,

Niu³

et al. 2018

Cell Physiol Biochem

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“…Authors suggest that PLCε can modulate p53 expression via its promoter methylation; however, there are no experimental data supporting this hypothesis. Interesting data has been obtained by Han et al who showed that microRNA-328 (miR-328) can reduce the expression of PLCε at both mRNA and protein levels in ESCC cell lines EC109 and EC9706 38. Not only miR-328, but also miR-145 can have the same impact on PLCε expression in ESCC cells, as shown by Cui et al 39.…”

Section: Digestive Tract Cancersmentioning

confidence: 89%

The controversial role of phospholipase C epsilon (PLCε) in cancer development and progression

Tyutyunnykova¹,

Телегеев²,

Dubrovska³

2017

J. Cancer

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The phospholipase C (PLC) enzymes are important regulators of membrane phospholipid metabolism. PLC proteins can be activated by the receptor tyrosine kinases (RTK) or G-protein coupled receptors (GPCR) in response to the different extracellular stimuli including hormones and growth factors. Activated PLC enzymes hydrolyze phosphoinositides to increase the intracellular level of Ca2+ and produce diacylglycerol, which are important mediators of the intracellular signaling transduction. PLC family includes 13 isozymes belonging to 6 subfamilies according to their domain structures and functions. Although importance of PLC enzymes for key cellular functions is well established, the PLC proteins belonging to the ε, ζ and η subfamilies were identified and characterized only during the last decade. As a largest known PLC protein, PLCε is involved in a variety of signaling pathways and controls different cellular properties. Nevertheless, its role in carcinogenesis remains elusive.The aim of this review is to provide a comprehensive and up-to-date overview of the experimental and clinical data about the role of PLCε in the development and progression of the different types of human and experimental tumors.

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“…In addition, PLCE1 expression was positively correlated with that of nuclear factor κB-related proteins in Kazakh ESCC patients [51], and negatively correlated with TP53 in HCC and ESCC cells and lung cancer [32, 52, 53]. Distinct microRNAs were shown to suppress PLCE1 expression and thereby affect cancer development and patient prognosis [44, 49, 54]. …”

Section: Discussionmentioning

confidence: 99%

PLCE1 polymorphisms and expression combined with serum AFP level predicts survival of HBV-related hepatocellular carcinoma patients after hepatectomy

et al. 2017

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Polymorphisms in the phospholipase C epsilon (PLCE) 1 gene play a crucial role in the development and progression of several types of cancer. The present study investigated the prognostic significance of PLCE1 gene polymorphisms and expression combined with serum α-fetoprotein (AFP) level in hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC). Single nucleotide polymorphisms were genotyped by sequencing DNA isolated from surgically resected tumor samples of 421 HBV-related HCC patients, and expression profiles were generated based on the GSE14520 dataset. A joint-effects analysis of PLCE1 haplotypes (Ars2274223Crs3765524; Grs2274223Trs3765524) with AFP level stratified at 20 ng/ml showed a significant association with overall survival(OS) of HBV-related HCC patients(log-rank P=0.0003). Patients with AC and GT haplotypes with AFP level ≥ 20 ng/ml had an increased risk of death as compared to those with the AC haplotype and AFP level < 20 ng/ml (adjusted P=0.029 and 0.041, respectively). Patients with the GT haplotype and AFP level < 20 ng/ml also had an increased risk of death, although with a non-significant P value (adjusted P=0.092). Joint-effects analysis of PLCE1 mRNA expression with serum AFP level stratified at 300 ng/ml was significantly associated with HBV-related HCC recurrence and OS. Our results demonstrate that PLCE1 haplotypes (including rs2274223 and rs3765524) and expression combined with serum AFP level may predict postoperative outcome of HBV-related HCC patients.

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MiR-328 suppresses the survival of esophageal cancer cells by targeting PLCE1

Cited by 43 publications

References 31 publications

Whole-Transcriptome Analysis of CD133+CD144+ Cancer Stem Cells Derived from Human Laryngeal Squamous Cell Carcinoma Cells

Whole-Transcriptome Analysis of CD133+CD144+ Cancer Stem Cells Derived from Human Laryngeal Squamous Cell Carcinoma Cells

The controversial role of phospholipase C epsilon (PLCε) in cancer development and progression

PLCE1 polymorphisms and expression combined with serum AFP level predicts survival of HBV-related hepatocellular carcinoma patients after hepatectomy

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