MiR-326 sponges TET2 triggering imbalance of Th17/Treg differentiation to exacerbate pyroptosis of hepatocytes in concanavalin A-induced autoimmune hepatitis

Zhang, Genglin; Wu, Sensen; Xia, Guangtao

doi:10.1016/j.aohep.2023.101183

Cited by 3 publications

(2 citation statements)

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“…Our findings demonstrate that LINC01270 attenuates NF-κB activation by regulating the expression of LDOC1 through the sequestration of miR-326 (Figure 7). Previous reports on miR-326′s effects on TET2 revealed its pro-inflammatory nature [16]. However, an anti-inflammatory role of miR-326 via the destabilization of TLR4 mRNA has also been reported [17].…”

Section: Discussionmentioning

confidence: 99%

“…In autoimmune-induced hepatitis, miR-326 inhibition provided beneficial effects by reducing hepatocyte pyroptosis and the release of proinflammatory cytokines. Inhibition of MIR-326 resulted in the upregulation of tet methylcytosine dioxygenase 2 (TET2), which is known to inhibit NF-κB activation [16]. In contrast, Wang et al [17] demonstrated that miR-326 has the ability to diminish sepsis-induced lung injury by acting as a sponge for TLR4 mRNA, consequently reducing NF-κB mediated inflammation.…”

Section: Introductionmentioning

confidence: 99%

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LINC01270 Regulates the NF-κB-Mediated Pro-inflammatory Response via the miR-326/LDOC1 Axis in THP-1 Cells

Arab,

Lim,

Suk

et al. 2024

Preprint

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Long intergenic noncoding (LINC)01270 is a 2278 bp transcript belonging to the intergenic subset of long noncoding (lnc)RNAs. Despite increased reports of LINC01270’s involvement in different diseases, evident research on its effects on inflammation is yet to be achieved. In the present study, we investigated the potential role of LINC01270 in modulating the inflammatory response in the human monocytic leukemia cell line THP-1. Lipopolysaccharide treatment upregulated LINC01270 expression, and siRNA-mediated suppression of LINC01270 enhanced NF-kB activity and the subsequent production of cytokines IL-6, IL-8, and MCP-1. Interestingly, the knockdown of LINC01270 downregulated expression of leucine zipper downregulated in cancer 1 (LDOC1), a novel NF-kB suppressor. An analysis of the LINC01270/micro-RNA (miRNA)/protein interactome profile identified miR-326 as a possible mediator. Synthetic RNA agents that perturb the interaction among LINC01270, miR-326, and LDOC1 mRNA mitigated the changes caused by LINC01270 knockdown in THP-1 cells. Additionally, a luciferase re-porter assay in HEK293 cells further confirmed that LINC01270 knockdown enhances NF-kB activation, while its overexpression has the opposite effect. This study provides insight into LINC01270’s role in modulating inflammatory responses to lipopolysaccharide stimulation in THP-1 cells via miR-326/LDOC1 axis which negatively regulates NF-kB activation.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%