miR-323-3p regulates the steroidogenesis and cell apoptosis in polycystic ovary syndrome (PCOS) by targeting IGF-1

Wang, Tianjuan; Liu, Yajing; Lv, Mingrong; Xing, Qinghe; Zhang, Zhiguo; He, Xiaojin; Xu, Yuping; Wei, Zhaolian; Cao, Yunxia

doi:10.1016/j.gene.2018.10.006

Cited by 52 publications

(38 citation statements)

References 46 publications

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“…A number of mRNA targets of miR-323a have been identified, and include insulin-like growth factor 1 (IGF1) 43 , tumor protein p73 (TP73) 44 , brain protein I3 (BRI3) 45 , and fragile X mental retardation 1 (FMR1) 46 . MiR-323a is located in a large cluster of 54 miRNAs, found within the imprinted DLK1-DIO3 locus on 14q32 (reviewed in 47 ).…”

Section: Relationship Between Mir-323-3p and Erbb4 In Micementioning

confidence: 99%

miR-323a regulates ERBB4 and is involved in depression

et al. 2020

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Major depressive disorder (MDD) is a complex and debilitating illness whose etiology remains unclear. Small RNA molecules, such as micro RNAs (miRNAs) have been implicated in MDD, where they display differential expression in the brain and the periphery. In this study, we quantified miRNA expression by small RNA sequencing in the anterior cingulate cortex and habenula of individuals with MDD and psychiatrically-healthy controls. Thirty-two miRNAs showed significantly correlated expression between the two regions (False Discovery Rate < 0.05), of which four, miR-204-5p, miR-320b, miR-323a-3p, and miR-331-3p, displayed upregulated expression in MDD. We assessed the expression of predicted target genes of differentially expressed miRNAs in the brain, and found that the expression of erb-b2 receptor tyrosine kinase 4 (ERBB4), a gene encoding a neuregulin receptor, was downregulated in both regions, and was influenced by miR-323a-3p in vitro. Finally, we assessed the effects of manipulating miRNA expression in the mouse ACC on anxiety-and depressive-like behaviors. Mice in which miR-323-3p was overexpressed or knocked-down displayed increased and decreased emotionality, respectively. Additionally, these mice displayed significantly downregulated and upregulated expression of Erbb4, respectively. Overall, our findings indicate the importance of brain miRNAs in the pathology of MDD, and emphasize the involvement of miR-323a-3p and ERBB4 in this phenotype. Future studies further characterizing miR-323a-3p and neuregulin signaling in depression are warranted.

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Section: Relationship Between Mir-323-3p and Erbb4 In Micementioning

confidence: 99%

miR-323a regulates ERBB4 and is involved in depression

et al. 2020

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“…In vivo, IGF-1 is also targeted by miRNAs (31,32). In polycystic ovary syndrome, IGF-1 is targeted by miR-323-3p and miR-323-3p/IGF-1 is involved in the regulation of steroid secretion and human cumulus cell function (33). miR-135a inhibits the proliferation and metastasis of lung cancer cells by targeting IGF-1 to inhibit the AKT pathway (34).…”

Section: Discussionmentioning

confidence: 99%

MicroRNA‑29‑3p regulates the β‑catenin pathway by targeting IGF1 to inhibit the proliferation of prolactinoma cells

Xia

et al. 2021

Mol Med Rep

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The present study aimed to analyze the effects and underlying mechanisms of microRNA (miR)-29-3p on the proliferation and secretory abilities of prolactinoma cells by targeting insulin-like growth factor (IGF)-1/β-catenin. The relationship between miR-29a-3p and the survival of prolactinoma cells was analyzed with the Kaplan-Meier method in reference to The Cancer Genome Atlas. The expression levels of miR-29a-3p and IGF-1 in MMQ and GH3 cells were detected. A dual-luciferase reporter gene assay was performed to verify the combination of miR-29a-3p and IGF-1. Cells were transfected with a miR-29a-3p mimic and/or IGF-1 pcDNA3.1 to analyze the effects on the proliferation, apoptosis and secretion of prolactin (PRL) and growth hormone (GH) of prolactinoma cells. The effects on β-catenin in the cytoplasm and nucleus were investigated by western blot analysis. The results showed that miR-29a-3p expression was low in MMQ and GH3 cells. Overexpression miR-29a-3p inhibited IGF-1 mRNA and protein expression. miR-29a-3p inhibited cell proliferation and PRL and GH expression, and promoted apoptosis by inhibiting IGF-1. Increasing the expression of miR-29a-3p increased β-catenin levels in the cytoplasm, whereas IGF-1 promoted β-catenin activation and entry into the nucleus, and reversed the inhibitory effects of miR-29a-3p on β-catenin. To conclude, miR-29a-3p inhibited the proliferation and secretory abilities of prolactinoma cells by inhibiting nuclear translocation of β-catenin via a molecular mechanism that is inseparable from IGF-1.

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“…The effect of different concentration of catechins (25,50, and 100 mg/kg) on the body weight, uterus weight, hormone secretion and insulin resistance were firstly examined in mice, 200 mg/kg metformin was used as a positive drug. Result showed that there were no significant changes in body weight to each group (Fig.…”

Section: Metformin or Catechins Treatment Reduces Uterine Weight Hormentioning

confidence: 99%

“…The aim of this study was to investigate the effects of catechins on PCOS mice induced by sex hormone disorders and insulin resistance. In the mouse model we used a combination of insulin and human chorionic gonadotropin (hCG) to induce PCOSlike features [25]. In this model, we focus on the effects of catechins extracts from oolong tea on PCOS-like symptoms, glucose metabolism, insulin resistance, and pathological changes in the ovaries, as well as on the changes of uterine inflammation-related molecules, endometrial matrix degradation, and uterine STAT3 signaling in mice.…”

Section: Introductionmentioning

confidence: 99%

Catechins from oolong tea improve uterine defects by inhibiting STAT3 signaling in polycystic ovary syndrome mice

Hong

et al. 2020

Chin Med

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Background It is showed that inflammation is causative factor for PCOS, leading to a decline in ovarian fertility. Previous studies have reported that tea consumption can reduce the incidence of ovarian cancer. We speculate that catechins from oolong tea (Camellia sinensis (L.) O. Kuntze) may have a potential therapeutic effect on PCOS. This study aims to investigate the effects of oolong tea catechins on the uterus of polycystic ovary syndrome (PCOS) mice induced by insulin combined with human chorionic gonadotropin (hCG). Methods Sixty female mice were divided into 6 groups (n = 10): model, model + Metformin 200 mg/kg, model + catechins 25 mg/kg, model + catechins 50 mg/kg, and model + catechins 100 mg/kg. Another forty female mice were divided into 4 groups (n = 10): control, control + catechins 100 mg/kg, model, and model + catechins 100 mg/kg. Ovarian and uterine weight coefficients, sex hormone levels, glucose metabolism and insulin resistance, and ovarian and uterine pathology were examined. Changes in NF-κB-mediated inflammation, MMP2 and MMP9 expressions, and STAT3 signaling were evaluated in the uterus of mice. Results Catechins could effectively reduce the ovarian and uterine organ coefficients, reduce the levels of E2, FSH and LH in the blood and the ratio of LH/FSH, and improve glucose metabolism and insulin resistance in PCOS mice induced by insulin combined with hCG. In addition, catechins could significantly down-regulated the expression of p-NF-κB p65 in the uterus and the protein expressions of the pro-inflammatory factors (IL-1β, IL-6, and TNF-α). The expressions of mmp2 and mmp9 associated with matrix degradation in uterine tissue were also significantly down-regulated by catechins. Further, catechins significantly reduced the expression of p-STAT3 and increased the expression of p-IRS1 and p-PI3K in the uterus of PCOS mice. Conclusion Catechins from oolong tea can alleviate ovarian dysfunction and insulin resistance in PCOS mice by inhibiting uterine inflammation and matrix degradation via inhibiting p-STAT3 signaling.

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miR-323-3p regulates the steroidogenesis and cell apoptosis in polycystic ovary syndrome (PCOS) by targeting IGF-1

Cited by 52 publications

References 46 publications

miR-323a regulates ERBB4 and is involved in depression

miR-323a regulates ERBB4 and is involved in depression

MicroRNA‑29‑3p regulates the β‑catenin pathway by targeting IGF1 to inhibit the proliferation of prolactinoma cells

Catechins from oolong tea improve uterine defects by inhibiting STAT3 signaling in polycystic ovary syndrome mice

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