miR-322 promotes the differentiation of embryonic stem cells into cardiomyocytes

Li, Kai; Peng, Xiaoping; Luo, Lun

doi:10.1007/s10142-023-01008-0

Cited by 2 publications

(2 citation statements)

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“…GAPDH or U6 was selected as the loading control of mRNA or miRNA. 2 −△△ct was used for data analysis (Liu et al 2023 ).…”

Section: Methodsmentioning

confidence: 99%

miR-21-5p inhibits the growth of brain glioma cells through regulating the glycolysis mediated by PFKFB2

Zhang,

Liu

2023

Funct Integr Genomics

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Brain glioma is a common gynecological tumor. MicroRNA (miRNA) plays a very important role in the pathogenesis and development of tumors. It was found that glycolysis played important regulatory roles in tumor growth. The present study aims to investigate the expression pattern of miR-21-5p in brain glioma cells. We examined miR-21-5p and PFKFB2 levels in brain glioma cells via qRT-PCR. Then we performed CCK-8 and Transwell migration assays and determined glucose uptake and lactose production to unveil the properties of miR-21-5p in invasion, cell viability, along with glycolysis in brain glioma cells. Luciferase activity assay was implemented to elucidate if PFKFB2 was a miR-21-5p target gene. Western blotting and qRT-PCR were executed to further validate that miR-21-5p targeted PFKFB2. We repeated these functional assays to observe whether miR-21-5p could impede the function of PFKFB2. qRT-PCR signified that miR-21-5p was elevated in brain glioma tissues in contrast to matching adjacent normal tissues. Functional assays disclosed that elevation of miR-21-5p promoted cell viability, invasion, together with glycolysis. Luciferase assay indicated that PFKFB2 was a miR-21-5p target gene. Moreover, miR-21-inhibit could hinder cell viability, invasion, and glycolysis triggered by overexpression of PFKFB2 in brain glioma cells. miR-21-5p level is elevated in brain glioma and can impede brain glioma cell growth via regulating the glycolysis mediated by PFKFB2, thus is a potential target of treating brain glioma. Graphical abstract

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“…GAPDH or U6 was selected as the loading control of mRNA or miRNA. 2 −△△ct was used for data analysis (Liu et al 2023 ).…”

Section: Methodsmentioning

confidence: 99%

miR-21-5p inhibits the growth of brain glioma cells through regulating the glycolysis mediated by PFKFB2

Zhang,

Liu

2023

Funct Integr Genomics

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“…[ 8 , 9 ]. CELF1 is involved in many body homeostasis such as development of embryonic and heart, differentiation of bone and adipose tissue, as well as formation germ cells [ 10 – 12 ]. The aberrant expression of CELF1 leads to multiple diseases such as myotonic dystrophy type 1 (DM1) [ 13 ], myocardial hypertrophy [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Curriculum vitae of CUG binding protein 1 (CELF1) in homeostasis and diseases: a systematic review

Qin,

Shi,

Chen

et al. 2024

Cell Mol Biol Lett

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RNA-binding proteins (RBPs) are kinds of proteins with either singular or multiple RNA-binding domains (RBDs), and they can assembly into ribonucleic acid–protein complexes, which mediate transportation, editing, splicing, stabilization, translational efficiency, or epigenetic modifications of their binding RNA partners, and thereby modulate various physiological and pathological processes. CUG-BP, Elav-like family 1 (CELF1) is a member of the CELF family of RBPs with high affinity to the GU-rich elements in mRNA, and thus exerting control over critical processes including mRNA splicing, translation, and decay. Mounting studies support that CELF1 is correlated with occurrence, genesis and development and represents a potential therapeutical target for these malignant diseases. Herein, we present the structure and function of CELF1, outline its role and regulatory mechanisms in varieties of homeostasis and diseases, summarize the identified CELF1 regulators and their structure–activity relationships, and prospect the current challenges and their solutions during studies on CELF1 functions and corresponding drug discovery, which will facilitate the establishment of a targeted regulatory network for CELF1 in diseases and advance CELF1 as a potential drug target for disease therapy. Graphical Abstract

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miR-322 promotes the differentiation of embryonic stem cells into cardiomyocytes

Cited by 2 publications

References 50 publications

miR-21-5p inhibits the growth of brain glioma cells through regulating the glycolysis mediated by PFKFB2

miR-21-5p inhibits the growth of brain glioma cells through regulating the glycolysis mediated by PFKFB2

Curriculum vitae of CUG binding protein 1 (CELF1) in homeostasis and diseases: a systematic review

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