miR-320a functions as a suppressor for gliomas by targeting SND1 and β-catenin, and predicts the prognosis of patients

Li, Huining; Yu, Lin; Liu, Jing; Bian, Xiu‐Wu; Shi, Cuijuan; Sun, Cuiyun; Zhou, Xuexia; Yi, Wen; Hua, Di; Zhao, S. J.; Ren, Linlin; An, Tongqing; Luo, Wenjun; Wang, Qian; Yu, Shizhu

doi:10.18632/oncotarget.14975

Cited by 47 publications

(43 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, LAMP‐1 is shown to be directly regulated by miRNA‐320a in prostate cancer. Interestingly, the expression of the same miRNA was decreased in both human glioma tissues and cell lines . Furthermore, silencing of LAMP‐1 inhibited proliferation, migration and invasion of cancer cells .…”

Section: Discussionmentioning

confidence: 99%

LAMP‐1 gene is overexpressed in high grade glioma

Sarafian

Koev²,

Mehterov

et al. 2018

APMIS

View full text Add to dashboard Cite

High-grade gliomas (HGG) are the most frequent brain tumors in adults. Glioblastoma multiforme (GBM) is their most aggressive form resistant to therapy. It was shown that inhibition of autophagy reduced GBM development and autophagy interfering agents are regarded as a new strategy to fight glioma cells. The lysosome-associated membrane proteins (LAMPs) display differential expression particularly in cancer. There are few data on their expression and especially on their molecular profile. The aim of the present study is to investigate the expression of LAMP-1 and LAMP-2 genes and proteins in HGG. Newly diagnosed patients with HGG and healthy controls were examined by immunohistochemistry and qPCR for both protein and mRNA levels of LAMP-1 and LAMP-2. The transcriptional activity of LAMP-1 in HGG was significantly higher compared to normal brain and to LAMP-2. The two glycoproteins were detected in the cytosol of tumor cells with varying intensity, LAMP-1 showing again enhanced expression. In conclusion, novel data on LAMP-1 overexpression in HGG are presented suggesting involvement of this gene and protein in cell adhesion and tumor progression. These findings might help the elucidation of the complex biological role of the multifunctional LAMPs proteins and to predict novel therapeutic targets in lysosomes.

show abstract

Section: Discussionmentioning

confidence: 99%

LAMP‐1 gene is overexpressed in high grade glioma

Sarafian

Koev²,

Mehterov

et al. 2018

APMIS

View full text Add to dashboard Cite

show abstract

“…Then, 5 μm continuous sections were prepared for IHC of SRSF1 and Ki-67. Histopathological diagnoses were made independently by 2 neuropathologists according to the 2016 WHO classification of central nervous system tumors (39). Sanger sequencing was adopted to verify the gene type of IDH1/2 (39).…”

Section: Methodsmentioning

confidence: 99%

Splicing factor SRSF1 promotes gliomagenesis via oncogenic splice-switching of MYO1B

Zhou¹,

Wang²,

Li³

et al. 2019

Journal of Clinical Investigation

Self Cite

116

100

View full text Add to dashboard Cite

“…Dysregulation of miRNAs has been reported in almost all types of malignancy, including glioma (11), gastric cancer (12), lung cancer (13), ovarian cancer (14) and bladder cancer (15). miRNAs are involved in tumourigenesis and tumour development through participation in the regulation of diverse biological processes, including cell proliferation, cycle, apoptosis, angiogenesis, migration, invasion, metastasis and epithelial-mesenchymal transition (16)(17)(18). Therefore, further investigation into the biological role of GBM-associated miRNAs may aid the identification of novel therapeutic approaches for patients with this disease.…”

Section: Introductionmentioning

confidence: 99%

microRNA‑574 inhibits cell proliferation and invasion in glioblastoma multiforme by directly targeting zinc finger E‑box‑binding homeobox 1

Mao

Wei

et al. 2018

Mol Med Report

View full text Add to dashboard Cite

Accumulated evidence has demonstrated that dysregulation of microRNAs (miRNAs) contributes to tumourigenesis and tumour development of glioblastoma multiforme (GBM). Therefore, miRNAs may be promising candidates in the development of prognosis biomarkers and effective therapeutic targets for patients with GBM. A number of studies have reported that miRNA‑574 (miR‑574) is aberrantly expressed in multiple types of human cancers. However, the expression pattern, biological functions and molecular mechanism of miR‑574 in GBM are yet to be elucidated. Therefore, the present study aimed to determine the expression level and biological functions of miR‑574 in GBM and the underlying molecular mechanisms. In the present study, miR‑574 levels were measured by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) and were demonstrated to be significantly downregulated in human GBM tissues and cell lines. Functional experiments indicated that restored expression of miR‑574 using mimics led to the inhibition of the cell proliferation and invasion of GBM cells, as determined by Cell Counting kit‑8 and Matrigel invasion assays, respectively. In addition, bioinformatics analysis predicted that zinc finger E‑box‑binding homeobox 1 (ZEB1) may be a target of miR‑574. Subsequent RT‑qPCR, western blot analysis and luciferase reporter assays confirmed that ZEB1 was a direct target of miR‑574 in GBM. Additionally, ZEB1 was demonstrated to be upregulated and inversely correlated with miR‑574 expression in clinical GBM tissues. Rescue experiments demonstrated that overexpression of ZEB1 attenuated the inhibitory effects of miR‑574 on the proliferation and invasion of GBM cells. Overall, the results of the present study highlighted the potential tumour inhibitory roles of miR‑574 in GBM, thereby indicating that miR‑574 may be a novel and efficient therapeutic target for the treatment of patients with GBM.

show abstract

miR-320a functions as a suppressor for gliomas by targeting SND1 and β-catenin, and predicts the prognosis of patients

Cited by 47 publications

References 40 publications

LAMP‐1 gene is overexpressed in high grade glioma

LAMP‐1 gene is overexpressed in high grade glioma

Splicing factor SRSF1 promotes gliomagenesis via oncogenic splice-switching of MYO1B

microRNA‑574 inhibits cell proliferation and invasion in glioblastoma multiforme by directly targeting zinc finger E‑box‑binding homeobox 1

Contact Info

Product

Resources

About