miR-317-3p and miR-283-5p Play a Crucial Role in Regulating the Resistance to Indoxacarb in Spodoptera frugiperda by Targeting GSTs4

Abstract

Spodoptera frugiperda is primarily controlled through chemical insecticides. Our RNA-seq data highlight the overexpression of GSTs4 in indoxacarb-resistant S. frugiperda. However, the exact role of GSTs4 in indoxacarb resistance and its regulatory mechanisms remains elusive. Therefore, we investigated the functional role of GSTs4 in S. frugiperda and explored the underlying post-transcriptional regulatory mechanisms. GSTs4 was highly overexpressed (27.6-fold) in the indoxacarb-resistant strain, and GSTs4 silencing significantly increases the susceptibility of S. frugiperda to indoxacarb, increasing mortality by 27.3%. miR-317-3p and miR-283-5p can bind to the 3′UTR of GSTs4, and the targeting relationship was confirmed by dual-luciferase reporter assays. Injecting miR-317-3p and miR-283-5p agomirs reduces GSTs4 levels by 64.8 and 42.3%, respectively, resulting in an increased susceptibility of S. frugiperda to indoxacarb. Conversely, the administration of miR-317-3p and miR-283-5pantagomirs increases GSTs4 expression and reduces larval susceptibility to indoxacarb. These findings demonstrate that miR-317-3p and miR-283-5p contribute to indoxacarb resistance in S. frugiperda by regulating the overexpression of GSTs4.

Functional Analysis of Insecticide Inhibition and Metabolism of Six Glutathione S-Transferases in the Rice Stem Borer, Chilo suppressalis

“…As a major kind of detoxifying enzyme, GSTs were confirmed to be involved in the resistance of various insects and mites to different insecticides, such as BdGSTe2 and BdGSTe4 in malathion-resistant Bactrocera dorsalis, BtGSTd7 in imidacloprid-resistant Bemisia tabaci, TuGSTd05 in cyflumetofen-resistant Tetranychus urticae, and AfGSTe2 in DDT and permethrin-resistant Anopheles funestus . Furthermore, a variety of GST genes were overexpressed or induced by insecticide exposure in insects, which might contribute to the increased detoxification metabolism of insects to xenobiotics. ,, In our previous study of GSTs in C. suppressalis, the toxicity of abamectin to C.…”