MiR-3120 Is a Mirror MicroRNA That Targets Heat Shock Cognate Protein 70 and Auxilin Messenger RNAs and Regulates Clathrin Vesicle Uncoating

Scott, Helen L.; Howarth, Joanna; Lee, Youn‐Bok; Wong, Liang‐Fong; Bantounas, Ioannis; Phylactou, Leonidas A.; Verkade, Paul; Uney, James B.

doi:10.1074/jbc.m111.326041

Cited by 46 publications

(51 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition to miR emergence, a new miR, miR3120, was revealed by high-throughput sequencing in human, rat, cow and in the Jamaican fruit bat 52 and the miR3120 gene overlaps miR214 with nearly complete overlap but on the opposite strand, and is thus categorized as a mirrormiR. 53,54 Expression of mirror-miRs has been demonstrated in Drosophila, 53 but to our knowledge, miR3120 is the only experimentally documented mirror-miR in vertebrates. 54 As one would expect from an intronic miR, miR3120 has been shown to be co-expressed with and to regulate important aspects of cellular function similar to its same-stranded host coding gene Dnm3.…”

Section: Resultsmentioning

confidence: 99%

“…53,54 Expression of mirror-miRs has been demonstrated in Drosophila, 53 but to our knowledge, miR3120 is the only experimentally documented mirror-miR in vertebrates. 54 As one would expect from an intronic miR, miR3120 has been shown to be co-expressed with and to regulate important aspects of cellular function similar to its same-stranded host coding gene Dnm3. 54 In teleost fish, five to six dynamin genes have already been annotated ( Table 1; Table S1).…”

Section: Resultsmentioning

confidence: 99%

“…54 As one would expect from an intronic miR, miR3120 has been shown to be co-expressed with and to regulate important aspects of cellular function similar to its same-stranded host coding gene Dnm3. 54 In teleost fish, five to six dynamin genes have already been annotated ( Table 1; Table S1). Orthology plots between human and zebrafish dynamin loci show that each human DNM gene has two coorthologs in zebrafish ( Fig.…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Evolution of themiR199-214cluster and vertebrate skeletal development

2014

View full text Add to dashboard Cite

The miR199 and miR214 genes occupy an intronic cluster located on the opposite strand of the Dynamin3 gene. These miRNAs play major roles in a broad variety of developmental processes and diseases, including skeletal development and several types of cancer. In the work reported here, we first deciphered the origin of the miR199 and miR214 families by following evolution of miR paralogs and their host Dynamin paralogs. We then examined the expression patterns of miR199 and miR214 in developing zebrafish embryos and demonstrated their regulation through a common primary transcript. Results suggest an evolutionarily conserved regulation across vertebrate lineages. Our expression study showed predominant expression patterns for both miR in tissues surrounding developing craniofacial skeletal elements consistent with expression data in mouse and human, thus indicating a conserved role of miR199 and miR214 in vertebrate skeletogenesis.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Evolution of themiR199-214cluster and vertebrate skeletal development

2014

View full text Add to dashboard Cite

show abstract

“…Of note, miR-214 is known to target phosphatase and tensin homolog (PTEN), which interacts with DNM and regulates receptor recycling (Yang et al, 2008). More interesting, the mirror miRNA miR-3120, which is fully the complement of miR-214 in the DNM3 intron, is co-expressed with its host gene mRNA and regulates uncoating of clathrin-coated vesicles by targeting Hsc70 and auxilin (Scott et al, 2012). These studies are in agreement with our hypothesis that DNM intronic miRNAs regulate important aspects of cellular functions that are similar to those regulated by its host gene.…”

Section: Discussionmentioning

confidence: 99%

The miR-199/DNM regulatory axis controls receptor-mediated endocytosis

Aranda

Canfrán‐Duque

Goedeke

et al. 2015

Journal of Cell Science

View full text Add to dashboard Cite

Small non-coding RNAs (microRNAs) are important regulators of gene expression that modulate many physiological processes; however, their role in regulating intracellular transport remains largely unknown. Intriguingly, we found that the dynamin (DNM) genes, a GTPase family of proteins responsible for endocytosis in eukaryotic cells, encode the conserved miR-199a and miR-199b family of miRNAs within their intronic sequences. Here, we demonstrate that miR-199a and miR-199b regulate endocytic transport by controlling the expression of important mediators of endocytosis such as clathrin heavy chain (CLTC), Rab5A, lowdensity lipoprotein receptor (LDLR) and caveolin-1 (Cav-1). Importantly, miR-199a-5p and miR-199b-5p overexpression markedly inhibits CLTC, Rab5A, LDLR and Cav-1 expression, thus preventing receptor-mediated endocytosis in human cell lines (Huh7 and HeLa). Of note, miR-199a-5p inhibition increases target gene expression and receptor-mediated endocytosis. Taken together, our work identifies a new mechanism by which microRNAs regulate intracellular trafficking. In particular, we demonstrate that the DNM, miR-199a-5p and miR-199b-5p genes act as a bifunctional locus that regulates endocytosis, thus adding an unexpected layer of complexity in the regulation of intracellular trafficking.

show abstract

“…In addition, depletion of miR-320 enhances levels of its downstream target HSPB6/ HSP20 to protect cardiomyocytes from I/R-induced death (Ren et al 2009). It has also been shown that miR-3120 plays a role in regulating constitutive levels of HSPA8/Hsc70 in neuronal cells by targeting multiple conserved sites within its 3′UTR (Scott et al 2012). Because HSPA8/Hsc70 catalyzes the uncoating of clathrin-coated vesicles, miR-3120 overexpression prevents vesicle uncoating.…”

Section: Mirnas Target Heat Shock Protein Transcriptsmentioning

confidence: 99%

Non-coding RNAs turn up the heat: An emerging layer of novel regulators in the mammalian heat shock response

Place¹,

Noonan

2014

Cell Stress and Chaperones

View full text Add to dashboard Cite

The field of non-coding RNA (ncRNA) has expanded over the last decade following the discoveries of several new classes of regulatory ncRNA. A growing amount of evidence now indicates that ncRNAs are involved even in the most fundamental of cellular processes. The heat shock response is no exception as ncRNAs are being identified as integral components of this process. Although this area of research is only in its infancy, this article focuses on several classes of regulatory ncRNA (i.e., miRNA, lncRNA, and circRNA), while summarizing their activities in mammalian heat shock. We also present an updated model integrating the traditional heat shock response with the activities of regulatory ncRNA. Our model expands on the mechanisms for efficient execution of the stress response, while offering a more comprehensive summary of the major regulators and responders in heat shock signaling. It is our hope that much of what is discussed herein may help researchers in integrating the fields of heat shock and ncRNA in mammals.

show abstract

MiR-3120 Is a Mirror MicroRNA That Targets Heat Shock Cognate Protein 70 and Auxilin Messenger RNAs and Regulates Clathrin Vesicle Uncoating

Cited by 46 publications

References 30 publications

Evolution of themiR199-214cluster and vertebrate skeletal development

Evolution of themiR199-214cluster and vertebrate skeletal development

The miR-199/DNM regulatory axis controls receptor-mediated endocytosis

Non-coding RNAs turn up the heat: An emerging layer of novel regulators in the mammalian heat shock response

Contact Info

Product

Resources

About