MiR-31-5p acts as a tumor suppressor in renal cell carcinoma by targeting cyclin-dependent kinase 1 (CDK1)

Li, Yawen; Quan, Jing; Chen, Fangfang; Pan, Xiang; Zhuang, Changshui; Xiong, Tiefu; Zhuang, Cheng‐Le; Li, Jianfa; Huang, Xinbo; Ye, Jing; Zhang, Fangting; Zeng, Zhen; Gui, Yaoting

doi:10.1016/j.biopha.2018.12.102

Cited by 38 publications

(32 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[ 30 ] In addition, overexpression of miR-31–5p significantly inhibited renal cell carcinoma progression in vitro through targeting CDK1. [ 31 ] Similar association was identified in the present study that CDK1 was upregulated in HCC and higher expression of CDK1 correlated with worse survival of HCC patients. Taken together, these data indicated that CDK1 may act as a potential biomarker for predicting the survival in HCC.…”

Section: Discussionsupporting

confidence: 89%

Screening of significant biomarkers with poor prognosis in hepatocellular carcinoma via bioinformatics analysis

et al. 2020

View full text Add to dashboard Cite

Hepatocellular carcinoma (HCC) is a malignant tumor with unsatisfactory prognosis. The abnormal genes expression is significantly associated with initiation and poor prognosis of HCC. The aim of the present study was to identify molecular biomarkers related to the initiation and development of HCC via bioinformatics analysis, so as to provide a certain molecular mechanism for individualized treatment of hepatocellular carcinoma. Three datasets (GSE101685, GSE112790, and GSE121248) from the GEO database were used for the bioinformatics analysis. Differentially expressed genes (DEGs) of HCC and normal liver samples were obtained using GEO2R online tools. Gene ontology term and Kyoto Encyclopedia of Gene and Genome (KEGG) pathway analysis were conducted via the Database for Annotation, Visualization, and Integrated Discovery online bioinformatics tool. The protein–protein interaction (PPI) network was constructed by the Search Tool for the Retrieval of Interacting Genes database and hub genes were visualized by Cytoscape. Survival analysis and RNA sequencing expression were conducted by UALCAN and Gene Expression Profiling Interactive Analysis. A total of 115 shared DEGs were identified, including 30 upregulated genes and 85 downregulated genes in HCC samples. P53 signaling pathway and cell cycle were the major enriched pathways for the upregulated DEGs whereas metabolism-related pathways were the major enriched pathways for the downregulated DEGs. The PPI network was established with 105 nodes and 249 edges and 3 significant modules were identified via molecular complex detection. Additionally, 17 candidate genes from these 3 modules were significantly correlated with HCC patient survival and 15 of 17 genes exhibited high expression level in HCC samples. Moreover, 4 hub genes (CCNB1, CDK1, RRM2, BUB1B) were identified for further reanalysis of KEGG pathway, and enriched in 2 pathways, the P53 signaling pathway and cell cycle pathway. Overexpression of CCNB1, CDK1, RRM2, and BUB1B in HCC samples was correlated with poor survival in HCC patients, which could be potential therapeutic targets for HCC.

show abstract

Section: Discussionsupporting

confidence: 89%

Screening of significant biomarkers with poor prognosis in hepatocellular carcinoma via bioinformatics analysis

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Nevertheless, the role of miR-31-5p in RCC pathogenesis is not without inconsistencies. A previous study suggested that miR-31-5p may act as a tumor suppressor in RCC by inhibiting proliferation, migration, and invasion via targeting cyclin-dependent kinase 1 (Li et al, 2019). Moreover, the expression of miR-31-5p has been reported to be significantly lower in RCC tissue and cell lines (Li et al, 2019).…”

Section: Circulating Mirnas As Predictive Biomarkers For Therapy Respmentioning

confidence: 99%

“…A previous study suggested that miR-31-5p may act as a tumor suppressor in RCC by inhibiting proliferation, migration, and invasion via targeting cyclin-dependent kinase 1 (Li et al, 2019). Moreover, the expression of miR-31-5p has been reported to be significantly lower in RCC tissue and cell lines (Li et al, 2019). Gamez-Pozo et al (2012) developed several predictive models of miRNA expression in the peripheral blood for resistance to sunitinib therapy in a prospective observational multicenter study including 38 metastatic RCC patients who received firstline treatment with sunitinib.…”

Section: Circulating Mirnas As Predictive Biomarkers For Therapy Respmentioning

confidence: 99%

Circulating Non-coding RNAs in Renal Cell Carcinoma—Pathogenesis and Potential Implications as Clinical Biomarkers

Barth

Drula

Ott

et al. 2020

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Liquid biopsy-the determination of circulating cells, proteins, DNA or RNA from biofluids through a "less invasive" approach-has emerged as a novel approach in all cancer entities. Circulating non-(protein) coding RNAs including microRNAs (miRNAs), long non-coding RNAs (lncRNAs), and YRNAs can be passively released by tissue or cell damage or actively secreted as cell-free circulating RNAs, bound to lipoproteins or carried by exosomes. In renal cell carcinoma (RCC), a growing body of evidence suggests circulating non-coding RNAs (ncRNAs) such as miRNAs, lncRNAs, and YRNAs as promising and easily accessible blood-based biomarkers for the early diagnosis of RCC as well as for the prediction of prognosis and treatment response. In addition, circulating ncRNAs could also play a role in RCC pathogenesis and progression. This review gives an overview over the current study landscape of circulating ncRNAs and their involvement in RCC pathogenesis as well as their potential utility as future biomarkers in RCC diagnosis and treatment.

show abstract

“…24 MiR-31-5p is considered to be a tumor suppressor in renal cell carcinoma because its overexpression can inhibit cell proliferation, invasion and cell cycle. 25 In bladder cancer, knockdown of circRNA-BPTF could increase miR-31-5p expression to restrain the proliferation and metastasis of bladder cancer. 26 Therefore, the anti-proliferation and antimetastasis effects of miR-31-5p might better elucidate the molecular mechanism of its interacting targets.…”

Section: Discussionmentioning

confidence: 99%

Long noncoding RNA LINC00511 regulates the proliferation, apoptosis, invasion and autophagy of trophoblast cells to mediate pre‐eclampsia progression through modulating the miR‐31‐5p/homeobox protein A7 axis

Dong

Zhang

Chen

et al. 2020

J of Obstet and Gynaecol

View full text Add to dashboard Cite

Aim Pre‐eclampsia (PE) is the usual complication during pregnancy. Long noncoding RNAs are essential regulatory factors in many diseases. Nevertheless, the role of LINC00511 in the development of PE has not been fully elucidated. Methods The expression of LINC00511, homeobox protein A7 (HOXA7) and miR‐31‐5p was determined by quantitative real‐time polymerase chain reaction. The levels of HOXA7 protein and autophagy‐related proteins were measured by western blot analysis. Besides, cell proliferation was evaluated using cell counting kit 8 and colony formation assays. The apoptosis and invasion of cells were detected via flow cytometry and transwell assay, respectively. Further, the interaction between miR‐31‐5p and LINC00511 or HOXA7 was confirmed by dual‐luciferase reporter assay. Results The LINC00511 and HOXA7 expression levels were decreased in placental tissues of PE patients, and the expression levels of both were positively correlated. LINC00511 knockdown suppressed proliferation, invasion and autophagy, while enhanced apoptosis in trophoblast cells. Meanwhile, the elevated HOXA7 expression promoted proliferation, invasion, autophagy, and inhibited the apoptosis of trophoblast cells. Besides, overexpression of HOXA7 also could reverse the effect of LINC00511 knockdown on the biological function of trophoblast cells. Further experiments confirmed that miR‐31‐5p could be sponged by LINC00511 and could target HOXA7. Also, miR‐31‐5p mimic could invert the promoting effect of LINC00511 overexpression on the biological function of trophoblast cells. Conclusion LINC00511 expression was crucial for maintaining the normal function of trophoblast cells, and the decreased its expression might promote the progress of PE, which might provide some theoretical strategies for reducing the development of PE.

show abstract

MiR-31-5p acts as a tumor suppressor in renal cell carcinoma by targeting cyclin-dependent kinase 1 (CDK1)

Cited by 38 publications

References 25 publications

Screening of significant biomarkers with poor prognosis in hepatocellular carcinoma via bioinformatics analysis

Screening of significant biomarkers with poor prognosis in hepatocellular carcinoma via bioinformatics analysis

Circulating Non-coding RNAs in Renal Cell Carcinoma—Pathogenesis and Potential Implications as Clinical Biomarkers

Long noncoding RNA LINC00511 regulates the proliferation, apoptosis, invasion and autophagy of trophoblast cells to mediate pre‐eclampsia progression through modulating the miR‐31‐5p/homeobox protein A7 axis

Contact Info

Product

Resources

About