Mir-30d increases intracellular survival of<i>Helicobacter pylori</i>through inhibition of autophagy pathway

Yang, Xiaojun; Si, Ruohuang; Liang, Yu-He; Ma, Bingqiang; Jiang, Zongyuan; Wang, Bin; Gao, Peng

doi:10.3748/wjg.v22.i15.3978

Cited by 43 publications

(29 citation statements)

References 36 publications

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“…BCL2-interacting coiled-coil protein (BECN1) and autophagy-related protein 12 (ATG12) are two important proteins that guide other autophagy proteins to the pre-autophagosomal membrane and subsequently facilitating phagophore elongation and autophagosomes maturation 17 . Two groups have reported that H. pylori infection increases miR-30b/d and that compromised autophagy by miR-30b/d promotes bacterial replication by targeting BECN1 and ATG12 17 , 122 . DNA damage-regulated autophagy modulator 2 (DRAM2) is shown to interact with BECN1 as a coordinator of autophagy activation.…”

Section: Significance Of Altered Mirnas In Bacterial Infectionmentioning

confidence: 99%

miRNAs reshape immunity and inflammatory responses in bacterial infection

Zhou

2018

Sig Transduct Target Ther

113

102

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Pathogenic bacteria cause various infections worldwide, especially in immunocompromised and other susceptible individuals, and are also associated with high infant mortality rates in developing countries. MicroRNAs (miRNAs), small non-coding RNAs with evolutionarily conserved sequences, are expressed in various tissues and cells that play key part in various physiological and pathologic processes. Increasing evidence implies roles for miRNAs in bacterial infectious diseases by modulating inflammatory responses, cell penetration, tissue remodeling, and innate and adaptive immunity. This review highlights some recent intriguing findings, ranging from the correlation between aberrant expression of miRNAs with bacterial infection progression to their profound impact on host immune responses. Harnessing of dysregulated miRNAs in bacterial infection may be an approach to improving the diagnosis, prevention and therapy of infectious diseases.

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Section: Significance Of Altered Mirnas In Bacterial Infectionmentioning

confidence: 99%

miRNAs reshape immunity and inflammatory responses in bacterial infection

Zhou

2018

Sig Transduct Target Ther

113

102

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“…Compromised autophagy promotes persistent infection of H. pylori (71). Additionally, ATG2B, ATG5, ATG12, BECN1 and BCL2 interacting protein 3 like are targets of miR-30d, leading to the repression of autophagy (72). miR-30d further promotes the intracellular survival of H. pylori during chronic infection (72).…”

Section: Chronic Infection Of H Pylori Can Inhibit Autophagymentioning

confidence: 99%

“…Additionally, ATG2B, ATG5, ATG12, BECN1 and BCL2 interacting protein 3 like are targets of miR-30d, leading to the repression of autophagy (72). miR-30d further promotes the intracellular survival of H. pylori during chronic infection (72). Furthermore, H. pylori infection induces methylation silencing of microtubule associated protein 1 light chain 3α variant 1, which may impair autophagy and facilitate gastric carcinogenesis (73).…”

Section: Chronic Infection Of H Pylori Can Inhibit Autophagymentioning

confidence: 99%

Molecular mechanism of Helicobacter�pylori‑induced autophagy in gastric cancer (Review)

Zhang

Chen

et al. 2019

Oncol Lett

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Helicobacter pylori (H. pylori) is a gram-negative pathogen that colonizes gastric epithelial cells. The drug resistance rates of H. pylori have dramatically increased, causing persistent infections. Chronic infection by H. pylori is a critical cause of gastritis, peptic ulcers and even gastric cancer. In host cells, autophagy is stimulated to maintain cellular homeostasis following intracellular pathogen recognition by the innate immune defense system. However, H. pylori-induced autophagy is not consistent during acute and chronic infection. Therefore, a deeper understanding of the association between H. pylori infection and autophagy in gastric epithelial cells could aid the understanding of the mechanisms of persistent infection and the identification of autophagy-associated therapeutic targets for H. pylori infection. The present review describes the role of H. pylori and associated virulence factors in the induction of autophagy by different signaling pathways during acute infection. Additionally, the inhibition of autophagy in gastric epithelial cells during chronic infection was discussed. The present review summarized H. pylori-mediated autophagy and provided insights into its mechanism of action, suggesting the induction of autophagy as a novel therapeutic target for persistent H. pylori infection.

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“…As a result, a total of 17 candidate target genes (including ATG12, ATg2B, ATg5, BCL9, BECN1, BNiP3L, CASP3, EZH2, gNAi2, gPR78, KPNB1, NoTCH1, RUNX2, SMAD1, SNAi1, SoCS1 and TP53) for which the interaction between miR-30d was validated experimentally by reporter assay, western blotting and qPCR, were collected from miRTarBase for miR-30d. Among these genes, a previous study of Esposito et al (10) found that miR-30d directly targeted the 3'-UTR of EZH2 in anaplastic thyroid carcinoma. Growing evidence also showed that EZH2 overexpression was associated with tumor aggressiveness and poor prognosis in patients with ESCC (16-18).…”

Section: Mir-30d Reduces Ezh2 Expression By Targeting the 3'-utrmentioning

confidence: 99%

“…Abnormal expression of miRNAs has been observed in multiple types of human cancers, implying that miRNAs may function as either oncogenes or tumor suppressors by the sequence-specific regulation of the corresponding target gene expression (9). miR-30d, a member of the miR-30 family, is localized in human chromosome 8q24.22 (10). Accumulating evidence has revealed that the aberrant expression of miR-30d contributes to cancer development and progression.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-30d inhibits the migration and invasion of human esophageal squamous cell carcinoma cells via the post-transcriptional regulation of enhancer of zeste homolog 2

Xie

Gao

et al. 2017

Oncology Reports

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The present study was carried out to investigate the expression pattern, clinical significance and biological functions of microRNA-30d (miR-30d) in esophageal carcinogenesis. Quantitative real-time PCR was performed to detect the expression levels of miR-30d in esophageal squamous cell carcinoma (ESCC) tissues and cell lines. Then, associations between miR-30d expression and various clinicopathological features of patients with ESCC were statistically evaluated. In addition, the effects of miR-30d on the migration and invasion of two human ESCC cell lines transfected with miRNA or co-transfected with miRNA mimics and the expression vector of its target gene were determined. The results revealed that the expression levels of miR-30d were markedly decreased in ESCC tissues and cell lines, comparing with the corresponding normal controls. Notably, reduced expression of miR-30d occurred more frequently in ESCC patients with positive lymph node metastasis, moderate-poor differentiation and advanced tumor-node-metastasis stage than those with negative features. Functionally, enforced expression of miR-30d was found to inhibit cell invasion and migration of the ESCC cell lines. Luciferase reporter assay identified enhancer of zeste homolog 2 (EZH2) as a direct target gene of miR-30d. The expression level of EZH2 mRNA was negatively correlated with the expression of miR-30d in the ESCC tissues. Moreover, the inhibitory effect of miR-30d on ESCC cell motility was reversed by EZH2 overexpression. Collectively, these findings provide convincing evidence that decreased expression of miR-30d may be implicated in esophageal carcinogenesis and progression. We also confirmed miR-30d as a tumor-suppressor which may inhibit cancer cell motility by targeting EZH2, a potential therapeutic target for ESCC.

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Mir-30d increases intracellular survival ofHelicobacter pylorithrough inhibition of autophagy pathway

Abstract: Mir-30d increases intracellular survival of H. pylori in gastric epithelial cells through inhibition of multiple core proteins in the autophagy pathway.

Cited by 43 publications

References 36 publications

miRNAs reshape immunity and inflammatory responses in bacterial infection

miRNAs reshape immunity and inflammatory responses in bacterial infection

Molecular mechanism of Helicobacter�pylori‑induced autophagy in gastric cancer (Review)

MicroRNA-30d inhibits the migration and invasion of human esophageal squamous cell carcinoma cells via the post-transcriptional regulation of enhancer of zeste homolog 2

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