Mir-30B-5P Inhibits Proliferation and Promotes Apoptosis of Medulloblastoma Cells via Targeting Myb Proto-Oncogene like 2 (Mybl2)

Cheng, Xu; He, Zixia; Lin, Chao; Shen, Zhipeng

doi:10.1136/jim-2020-001354

Cited by 12 publications

(5 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Overexpression of MYBL2 correlates with poor prognosis in numerous cancers [ 43 ], including breast cancer [ 44 , 45 , 46 ]. Recent studies have shown that MYBL2 was negatively regulated by mir-30b-5p in medulloblastoma [ 47 ]. NAV1 was reported to be significantly hypomethylated in ER+/PR+ breast cancers [ 48 ].…”

Section: Resultsmentioning

confidence: 99%

Circulating Exosomal microRNAs as Predictive Biomarkers of Neoadjuvant Chemotherapy Response in Breast Cancer

et al. 2022

View full text Add to dashboard Cite

Background: Neoadjuvant chemotherapy (NACT) is an increasingly used approach for treatment of breast cancer. The pathological complete response (pCR) is considered a good predictor of disease-specific survival. This study investigated whether circulating exosomal microRNAs could predict pCR in breast cancer patients treated with NACT. Method: Plasma samples of 20 breast cancer patients treated with NACT were collected prior to and after the first cycle. RNA sequencing was used to determine microRNA profiling. The Cancer Genome Atlas (TCGA) was used to explore the expression patterns and survivability of the candidate miRNAs, and their potential targets based on the expression levels and copy number variation (CNV) data. Results: Three miRNAs before that NACT (miR-30b, miR-328 and miR-423) predicted pCR in all of the analyzed samples. Upregulation of miR-127 correlated with pCR in triple-negative breast cancer (TNBC). After the first NACT dose, pCR was predicted by exo-miR-141, while miR-34a, exo-miR182, and exo-miR-183 predicted non-pCR. A significant correlation between the candidate miRNAs and the overall survival, subtype, and metastasis in breast cancer, suggesting their potential role as predictive biomarkers of pCR. Conclusions: If the miRNAs identified in this study are validated in a large cohort of patients, they might serve as predictive non-invasive liquid biopsy biomarkers for monitoring pCR to NACT in breast cancer.

show abstract

Section: Resultsmentioning

confidence: 99%

Circulating Exosomal microRNAs as Predictive Biomarkers of Neoadjuvant Chemotherapy Response in Breast Cancer

et al. 2022

View full text Add to dashboard Cite

show abstract

“…MiR-30b-5p exhibited both tumor suppressor and tumor promotion functions in various tumors. A grow body of research reported that miR-30b-5p suppressed malignant behaviors of esophageal squamous cell carcinoma, malignant glioma, liver cancer, renal cell carcinoma [35][36][37][38][39] . However, Wu et al 40 found that miR-30b-5p promoted the proliferation, migration and invasion of breast cancer cells.…”

Section: Discussionmentioning

confidence: 99%

Hypoxic pancreatic cancer derived exosomal miR-30b-5p promotes tumor angiogenesis by inhibiting GJA1 expression

Chen¹,

Wang²,

Liu³

et al. 2022

Int. J. Biol. Sci.

View full text Add to dashboard Cite

Purpose: Most patients with pancreatic ductal adenocarcinoma (PDAC) have vascular invasion and metastasis, leading to low surgical resection rate and dismal prognosis. Tumor angiogenesis is related to vascular invasion and metastasis. However, anti-angiogenesis therapeutic effects in PDAC are limited. Therefore, it is imperative to explore molecular mechanism of angiogenesis in PDAC. Experimental Design: scRNA-seq data were utilized to delineatetranscriptional profiles of endothelial cells in PDAC. The in vitro and vivo angiogenesis models were used to explore the role of PDAC derived exosomes under hypoxic condition in tumor angiogenesis. Results: Endothelial cells in PDAC had distinct gene expression profiles compared with normal pancreas. The marker genes of endothelial cells in PDAC were enriched for hypoxia and angiogenesis. MiR-30b-5p were significantly enriched in hypoxic PDAC cells derived exosomes, which could be transferred to HUVEC, resulting in the upregulation of miR-30b-5p. Hypoxic PDAC cells derived exosomes could promote tube formation and endothelial cells migration via miR-30b-5p mediated downregulation of gap junction protein GJA1. Moreover, hypoxic PDAC cells derived exosomes increased new microvascular density in vivo. Patients with PDAC had higher levels of total miR-30b-5p and exosomal miR-30b-5p in peripheral blood plasma than healthy subjects. In addition, there were significant correlations for the levels of total miR-30b-5p or exosomal miR-30b-5p between peripheral blood plasma and portal vein plasma. Conclusions: Hypoxic PDAC cells derived exosomal miR-30b-5p promoted angiogenesis by inhibiting GJA1, and miR-30b-5p was a potential diagnostic marker for PDAC.

show abstract

“…Subsequently, by a series of bioinformatics analyses and experiments, we identified that miR-30b was the downstream target of MRPL23-AS1. miR-30b belongs to miR-30 family, is a well-studied tumor suppressor in various human cancer types, including colorectal cancer [ 18 ], gastric cancer [ 19 ], medulloblastoma [ 20 ], hepatocellular carcinoma [ 21 ], esophageal squamous cell carcinoma [ 22 ] and OS [ 23 ]. Here, we also detected the level of miR-30b in OS tissues and normal tissues, found that it was dramatically decreased in OS tissues, and this phenomenon was also observed in OS cell lines.…”

Section: Discussionmentioning

confidence: 99%

Long non-coding RNA (LncRNA) MRPL23-AS1 promotes tumor progression and carcinogenesis in osteosarcoma by activating Wnt/β-catenin signaling via inhibiting microRNA miR-30b and upregulating myosin heavy chain 9 (MYH9)

et al. 2020

View full text Add to dashboard Cite

Long non-coding RNA (LncRNA) contributes to the occurrence and development of osteosarcoma (OS), although the underlying mechanism is not clear. In the present study, we showed that lncRNA MRPL23-AS1 was remarkably increased in OS tissues and cell lines. Stable knockdown of MRPL23-AS1 evidently attenuated cell viability and invasive ability, meanwhile inhibited in vivo tumor growth and dissemination. In terms of mechanism, luciferase reporter, RNA pull-down and fluorescence in situ hybridization (FISH) assays showed that MRPL23-AS1 competitively interacted with miR-30b, increasing myosin heavy chain 9 (MYH9) expression, a trans- activator of β-catenin, resulting in the activation of Wnt/β-catenin pathway, thereby promoting OS tumorigenesis and metastasis. Importantly, high MRPL23-AS1 was positively correlated with MYH9, while conversely correlated with miR-30b, suggesting that the regulatory axis of MRPL23-AS1/miR-30b/MYH9 does exist in OS. Clinically, OS patients with high MRPL23-AS1 had larger tumor size, higher stage and easier metastasis than those with low MRPL23-AS1, moreover, MRPL23-AS1 was identified as an adverse prognostic factor for OS survival. In conclusion, our results show that MRPL23-AS1 is a key oncogenic lncRNA in OS, targeting of MRPL23-AS1 may be a promising treatment for OS patients.

show abstract

Mir-30B-5P Inhibits Proliferation and Promotes Apoptosis of Medulloblastoma Cells via Targeting Myb Proto-Oncogene like 2 (Mybl2)

Cited by 12 publications

References 44 publications

Circulating Exosomal microRNAs as Predictive Biomarkers of Neoadjuvant Chemotherapy Response in Breast Cancer

Circulating Exosomal microRNAs as Predictive Biomarkers of Neoadjuvant Chemotherapy Response in Breast Cancer

Hypoxic pancreatic cancer derived exosomal miR-30b-5p promotes tumor angiogenesis by inhibiting GJA1 expression

Long non-coding RNA (LncRNA) MRPL23-AS1 promotes tumor progression and carcinogenesis in osteosarcoma by activating Wnt/β-catenin signaling via inhibiting microRNA miR-30b and upregulating myosin heavy chain 9 (MYH9)

Contact Info

Product

Resources

About