miR-27b regulates myogenic proliferation and differentiation by targeting Pax3 in goat

Ling, Yinghui; Sui, Menghua; Zheng, Qi; Wang, Kang-Yan; Wu, Hao; Li, Wenyong; Liu, Yong; Chu, Mingxing; Fang, Fugui; Xu, Lei

doi:10.1038/s41598-018-22262-4

Cited by 31 publications

(31 citation statements)

References 36 publications

(33 reference statements)

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“…mir-27b could modulate the expression of target genes at the posttranscriptional level (25,28,29). in the present study, we hypothesized whether mir-27b regulated the progression of ali through specific target genes.…”

Section: Discussionmentioning

confidence: 80%

lncRNA CASC2 inhibits lipopolysaccharide‑induced acute lung injury via miR‑27b/TAB2 axis

et al. 2020

Mol Med Rep

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long non-coding rna (lncrna) cancer susceptibility candidate 2 (caSc2) has been reported to exert an important role in acute lung injury (ali). The present study aimed to investigate the potential underlying mechanism of caSc2 in ali progression. reverse transcription-quantitative Pcr was conducted to examine the expression of caSc2, microrna (mir/mirna)-27b and TGF-β activated kinase 1 and MaP3K7-binding protein 2 (TaB2) in a549 cells. cell viability and apoptosis were analyzed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and flow cytometry. enzyme-linked immunosorbent assay was used to measure the levels of inflammatory-related cytokines to assess the inflammatory response, including interleukin-1β (il-1β), il-6 and tumor necrosis factor α (TnF-α). The binding sites of mir-27b in caSc2 or TaB2 were predicted using lncBase or microT-cdS software, following which dual-luciferase reporter and rna binding protein immunoprecipitation assays were performed to confirm the target relationship between mir-27b and caSc2 or TaB2. The protein expression of TaB2 was detected by western blotting. The decreased viability, and increased apoptosis and inflammatory responses were attenuated by the accumulation of caSc2 in lipopolysaccharide (lPS)-stimulated a549 cells. caSc2 could directly bind to mir-27b in a549 cells. caSc2 protected a549 cells from lPS-triggered injury by downregulating mir-27b. TAB2 was a target of miR-27b in A549 cells. The influence of mir-27b depletion was reversed by the silencing of TaB2 in an ali cell model. caSc2 could increase the expression of TaB2 by serving as a competing endogenous rna of mir-27b in a549 cells. collectively, the results suggested that caSc2 attenuated lPS-induced injury in the ali cell model by modulating the mir-27b/TaB2 axis.

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Section: Discussionmentioning

confidence: 80%

lncRNA CASC2 inhibits lipopolysaccharide‑induced acute lung injury via miR‑27b/TAB2 axis

et al. 2020

Mol Med Rep

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“…Some studies have shown that miRNAs have an indispensable role in goat muscle development at different periods. For example, miR-27b was identified to inhibit the proliferation but promoted differentiation of skeletal muscle satellite via targeting PAX3 (Ling et al, 2018). And miR-487b-3p, which is significantly higher in the fetus than in adult, suppressed myoblast proliferation and differentiation by targeting IRS1 in skeletal muscle myogenesis (Wang et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

RNA-Seq Reveals miRNA Role Shifts in Seven Stages of Skeletal Muscles in Goat Fetuses and Kids

et al. 2020

Self Cite

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MicroRNAs (miRNAs) are indispensable for the regulation of skeletal muscle. We performed RNA sequencing (RNA-seq) to establish a comprehensive miRNA profiling of goats in seven stages, namely, 45-(F45), 65-(F65), 90-(F90), 120-(F120), and 135-day (F135) fetuses, newborn (B1), and 90-day-old (B90) kids. In total, 421 known miRNAs and 228 goat novel miRNAs were identified in the data, and the average abundance of 19 miRNAs in seven stages exceeds 10,000 reads per million. Furthermore, 420 differentially expressed miRNAs (DEmiRNAs) were identified in all comparison group at seven stages, 80 of which were uniquely differentially expressed in the B1 and B90 comparison groups. Pathway analysis indicated that this group was associated with the release of muscle hypertrophy and regulation of myoblast proliferation. Besides, 305 DEmiRNAs were clustered into three significantly enriched profiles (profiles 11, 16, and 19). Function analysis revealed that profile 16 was related to muscle hypertrophy and differentiation. Profile 11 was involved in multiple enzyme activities and metabolic processes in muscle cells. And profile 19 was involved in material transport and structural stability. Two highly expressed miRNAs and three key miRNAs (chi-miR-328-3p, chi-miR-767, and chi-miR-150) of these profiles were verified to be consistent with the data by quantitative real-time PCR. These results provided a catalog of goat muscleassociated miRNAs, allowing us to better understand the transformation of miRNA roles during mammalian muscle development.

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“…Surprisingly, in seven different mouse muscle differentiation, or regeneration systems, we failed to detect the fusion. Considering the importance of Pax3 during myogenesis in mice and other mammals [2,13], and that the fusion shares similar downstream targets with the parental Pax3, we propose that the formation of the fusion may be a mechanism to escape certain microRNA regulation of the wild-type PAX3. Several recent studies also suggest that fusion genes can bypass microRNA-mediated regulation via changes of the 3′-UTR.…”

Section: Discussionmentioning

confidence: 95%

“…PAX3-FOXO1 has been reported to bind to the similar sites as wild-type PAX3, suggesting that the fusion targets similar downstream genes as parental PAX3 [5]. Given the critical role of Pax3 in the myogenesis of mice and other mammals [2,13], we then conjectured the possibility that the fusion in humans may substitute for the role Pax3 plays during myogenesis in mice.…”

Section: Pax3-foxo1 Is Absent In Mousementioning

confidence: 96%

PAX3-FOXO1 escapes miR-495 regulation during muscle differentiation

Xie

Tang

et al. 2019

RNA Biology

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Pax3 plays an essential role in myogenesis. Previously, we found a tumor-signature chimeric fusion RNA, PAX3-FOXO1 also present during muscle differentiation, raising the possibility of its physiological role. Here we demonstrated that the fusion is needed transiently for muscle lineage commitment. Interestingly, the fusion ortholog was not found in seven mouse muscle differentiation/regeneration systems, nor in other stem cell differentiation systems of another three mammal species. We noticed that Pax3 is expressed at a much lower level in human stem cells, and during muscle differentiation than in other mammals. Given the fact that the fusion and the parental Pax3 share common downstream targets, we reasoned that forming the fusion may be a mechanism for human cells to escape certain microRNA regulation on Pax3. By sequence comparison, we identified 16 candidate microRNAs that may specifically target the human PAX3 3ʹUTR. We used a luciferase reporter assay, examined the microRNAs expression, and conducted mutagenesis on the reporters, as well as a CRISPR/Cas9 mediated editing on the endogenous allele. Finally, we identified miR-495 as a microRNA that specifically targets human PAX3. Examining several other fusion RNAs revealed that the human-specificity is not limited to PAX3-FOXO1. Based on these observations, we conclude that PAX3-FOXO1 fusion RNA is absent in mouse, or other mammals we tested, the fusion RNA is a mechanism to escape microRNA, miR-495 regulation in humans, and that it is not the only human-specific fusion RNA.

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miR-27b regulates myogenic proliferation and differentiation by targeting Pax3 in goat

Cited by 31 publications

References 36 publications

lncRNA CASC2 inhibits lipopolysaccharide‑induced acute lung injury via miR‑27b/TAB2 axis

lncRNA CASC2 inhibits lipopolysaccharide‑induced acute lung injury via miR‑27b/TAB2 axis

RNA-Seq Reveals miRNA Role Shifts in Seven Stages of Skeletal Muscles in Goat Fetuses and Kids

PAX3-FOXO1 escapes miR-495 regulation during muscle differentiation

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