MiR-278-3p regulates pyrethroid resistance in Culex pipiens pallens

Lei, Zhentao; Lv, Yuan; Wang, Weijie; Guo, Qin; Zou, Feifei; Hu, S. Jack; Fang, F.; Tian, Mengmeng; Liu, Bingqian; Liu, Xianmiao; Ma, Kai; Ma, Lei; Zhou, Dan; Zhang, Donghui; Sun, Yan; Shen, Bo; Zhu, Changliang

doi:10.1007/s00436-014-4236-7

Cited by 46 publications

(35 citation statements)

References 33 publications

(34 reference statements)

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“…In insects, most of the experimentally validated cytochrome P450 target genes and their interactions with miRNAs have been reported from C. pipiens pallens in deltamethrin‐resistant and susceptible strains. It has been reported that miR‐285, miR‐278‐3p, miR‐2, miR‐71 and miR‐13 regulate different members of the cytochrome P450 family, such as CYP6N23 , CYP6AG11 , CYP9J35 and CYP325BG3 (Lei et al ., ; Tian et al ., ; Guo et al ., ). Microinjection of miR‐285 mimic into adult mosquitoes significantly decreased the mortality rate in response to deltamethrin exposure, whereas miR‐278‐3p mimic increased mortality.…”

Section: Discussionmentioning

confidence: 99%

Involvement of microRNA miR‐2b‐3p in regulation of metabolic resistance to insecticides in Plutella xylostella

Etebari

Afrad

Tang

et al. 2018

Insect Molecular Biology

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The diamondback moth, Plutella xylostella, has developed extremely high levels of resistance to chlorantraniliprole and other classes of insecticides in the field. As microRNAs (miRNAs) play important roles in various biological processes through gene regulation, we examined the miRNA profile of P. xylostella in response to chlorantraniliprole exposure. RNA sequencing analysis showed that insecticide treatment caused significant changes in the abundance of some miRNAs. Increasing exposure time and insecticide concentration induced more dysregulated miRNAs in P. xylostella larvae. We also screened potential target genes for some of the differentially expressed miRNAs (such as miR-2b-3p, miR-14b-5p and let-7-5p), which may play important roles in insecticide resistance development. Exposure of P. xylostella larvae to chlorantraniliprole caused considerable overexpression in the transcript levels of potential target genes cytochrome P450 9f2 (CYP9F2) and 307a1 (CYP307a1). Application of miR-2b-3p and miR-14b-5p mimics significantly suppressed the relative transcript levels of CYP9F2 and CYP307a1, respectively, in a P. xylostella cell line. Furthermore, enrichment of P. xylostella diet with miR-2b-3p mimics significantly increased mortality in deltamethrin-resistant larvae when exposed to deltamethrin. The results suggest that miR-2b-3p may suppress CYP9F2 transcript levels in P. xylostella and consequently inhibit larval detoxification pathways. The findings provide an insight into possible role of miRNAs in regulation of metabolic resistance of insects to insecticides.

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Section: Discussionmentioning

confidence: 99%

Involvement of microRNA miR‐2b‐3p in regulation of metabolic resistance to insecticides in Plutella xylostella

Etebari

Afrad

Tang

et al. 2018

Insect Molecular Biology

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“…A homolog of bte-miR-278, upregulated by IAPV infection, is involved in energy homeostasis resulting in the regulation of levels of insulin16 and regulation of the detoxification enzyme P450s17. Homologs of bte-miR-13a and -13b upregulated by IAPV and SBPV, respectively, regulate the expression of juvenile hormone (JH)18.…”

Section: Resultsmentioning

confidence: 99%

Infections of virulent and avirulent viruses differentially influenced the expression of dicer-1, ago-1, and microRNAs in Bombus terrestris

Niu

Meeus

Coninck

et al. 2017

Sci Rep

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The microRNA (miRNA) pathway is well established to be involved in host-pathogen interactions. As key insect pollinators, bees are suffering from widely spreading viruses, especially honeybees and bumblebees. In order to better understand bee-virus interaction, we comparatively analyzed the involvement of the bumblebee miRNA pathway upon infection by two different viruses. In our setup, an avirulent infection is induced by slow bee paralysis virus (SBPV) and a virulent infection is induced by Israeli acute paralysis virus (IAPV). Our results showed the increased expressions of dicer-1 and ago-1 upon SBPV infection. There were 17 and 12 bumblebee miRNAs differentially expressed upon SBPV and IAPV infections, respectively. These results may indicate the involvement of the host miRNA pathway in bumblebee-virus interaction. However, silencing of dicer-1 did not influence the genome copy number of SBPV. Target prediction for these differentially expressed miRNAs showed their possible involvement in targeting viral genomic RNA and in the regulation of networks in bumblebee. Our study opens a new insight into bee-virus interaction meditated by host miRNAs.

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“…A deep‐sequencing‐based approach revealed a list of 28 modulated miRNAs between deltamethrin‐sensitive and deltamethrin‐resistant C. pipiens (Hong et al, ). A subsequent study identified miR‐278‐3p as a downregulated target in deltamethrin‐resistant mosquitoes as well as revealed its ability to regulate the expression of the cytochrome P450 CYP6AG11 and ultimately affect response to deltamethrin (Lei et al, ). miR‐285 was also associated with resistance to this compound in C. pipiens via regulation of cytochrome P450 CYP6N23 expression (Tian et al, ).…”

Section: Discussionmentioning

confidence: 99%

Differential expression of transcripts with potential relevance to chlorantraniliprole response in the Colorado potato beetle, Leptinotarsa decemlineata

Dumas

Sambou

Gaudet

et al. 2019

Arch Insect Biochem Physiol

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The Colorado potato beetle (Leptinotarsa decemlineata [Say])is an insect pest that can significantly harm potato plants worldwide. Control of this insect relies heavily on chemical insecticides such as chlorantraniliprole. Nevertheless, the complete molecular signature associated with response to this compound is lacking in L. decemlineata. In this study, amplification and quantification by qRT-PCR (quantitative reverse transcription-polymerase chain reaction) of targets relevant to chlorantraniliprole were undertaken in insects exposed to this chemical. This approach showed modulation of numerous cytochrome P450s, such as CYP350D1 and CYP4Q3, as well as upregulation of microRNAs (miRNAs), including miR-1-3p and miR-305-5p, in chlorantraniliproleexposed insects. Functional assessment of transcript targets predicted to be regulated by these miRNAs was performed and revealed their likely impact on transcriptional regulation. RNAi-based targeting of CYP350D1 notably provided preliminary evidence of its underlying implication for chlorantraniliprole response in L. decemlineata. Overall, this study strengthens the current knowledge of the molecular changes linked to chlorantraniliprole response in L. decemlineata and provides novel targets with potential relevance to chlorantraniliprole susceptibility in this insect pest of global relevance.

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MiR-278-3p regulates pyrethroid resistance in Culex pipiens pallens

Cited by 46 publications

References 33 publications

Involvement of microRNA miR‐2b‐3p in regulation of metabolic resistance to insecticides in Plutella xylostella

Involvement of microRNA miR‐2b‐3p in regulation of metabolic resistance to insecticides in Plutella xylostella

Infections of virulent and avirulent viruses differentially influenced the expression of dicer-1, ago-1, and microRNAs in Bombus terrestris

Differential expression of transcripts with potential relevance to chlorantraniliprole response in the Colorado potato beetle, Leptinotarsa decemlineata

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