miR-27 regulates mitochondrial networks by directly targeting the mitochondrial fission factor

Abstract

Mitochondrial morphology is dynamically regulated by forming small, fragmented units or interconnected networks, and this is a pivotal process that is used to maintain mitochondrial homeostasis. Although dysregulation of mitochondrial dynamics is related to the pathogenesis of several human diseases, its molecular mechanism is not fully elucidated. In this study, we demonstrate the potential role of miR-27 in the regulation of mitochondrial dynamics. Mitochondrial fission factor (MFF) mRNA is a direct target of miR-27, whose ectopic expression decreases MFF expression through binding to its 3′-untranslated region. Expression of miR-27 results in the elongation of mitochondria as well as an increased mitochondrial membrane potential and mitochondrial ATP level. Our results suggest that miR-27 is a novel regulator affecting morphological mitochondrial changes by targeting MFF.

Effects of miRNAs on myocardial apoptosis by modulating mitochondria related proteins

“…Molecular study found that miR‐761 binds with 3′‐UTR of MFF mRNA for its downregulation . MiR‐27 also targets 3′‐UTR of MFF mRNA to suppress its expression and this inhibition favours enhanced mitochondrial elongation and fusion . Another member of miRNA family, miR‐484, attenuate Fis1‐induced mitochondrial fission and apoptosis in cardiomyocytes by binding with the amino acid coding region of Fis‐1 and inhibition of its translation.…”

Chlamydia preserves the mitochondrial network necessary for replication via microRNA-dependent inhibition of fission

“…Furthermore, mitochondrial fission and the regulator of mitochondrial fission Drp1 are influenced by increased metabolic demands and apoptotic stress (Cereghetti et al, 2010;Rambold et al, 2011). Recently, it has come to light that the regulation of mitochondrial dynamics is also governed by miRNAs (Wang et al, 2011a;Tak et al, 2014).…”

microRNA-200a-3p enhances mitochondrial elongation by targeting mitochondrial fission factor

“…For reporter analysis, Enhanced green fluorescent protein (EGFP) reporter vectors were constructed by inserting 3′UTR region of MFF mRNA (1509–1778 bp) into pEGFP-C1 (BD Bioscience) (41). A mutant reporter lacking the binding sites for the miR-200a-3p seed region was generated by site-directed mutagenesis using a KOD-Plus-Mutagenesis Kit (Toyobo).…”