miR-26a-5p suppresses nasopharyngeal carcinoma progression by inhibiting PTGS2 expression

Cai, Binlin; Qu, Xian‐Jun; Kan, Dan; Luo, Yi

doi:10.1080/15384101.2022.2030168

Cited by 9 publications

(4 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Song [16] demonstrated that INSM1 modulates NPC response to radiation through a cell cyclin D1-dependent DNA repair pathway, suggesting a potential molecular target for NPC treatment. Cai [17] revealed that miR−26a−5p binds to the 3' untranslated region of PTGS2, reducing the protein level and inhibiting NPC growth. Lung [18] associated the SAA1 genotype with a higher NPC incidence, with SAA1.5/1.5 being a recessive susceptibility gene and SAA1.1 and SAA1.3 being dominant alleles with tumor suppressor phenotypes.…”

Section: Discussionmentioning

confidence: 99%

Identifying immune cell infiltration and diagnostic biomarkers for nasopharyngeal carcinoma through bioinformatic analysis

zhao,

Qiu,

2024

Preprint

View full text Add to dashboard Cite

Backgrounds: Explore the genomic and signaling pathways contributing to nasopharyngeal carcinoma, a prevalent and multifactorial cancer involving environmental, genetic, and EB virus factors. This study aims to identify potential genes associated with nasopharyngeal cancer and examine their relationship with immune cell infiltration. Methods: The Gene Expression Omnibus (GEO) database provided three sets of nasopharyngeal cancer data: two for training and one for validation. To identify candidate genes, a Protein-Protein Interaction (PPI) network was constructed using a string database and two machine learning algorithms: the Least Absolute Selection and Shrinkage Operator (LASSO) and the Supporting Vector Machine Resource Elimination (SVM-RFE). The external validation dataset assessed the proposed levels of expression of biomarkers and the diagnostic value of the test to evaluate its effectiveness. Receiver Operating Characteristic (ROC) curves were used to measure diagnostic performance, convolutional techniques in CIBERSORT were applied for immune cell infiltration analysis, and correlation analysis was conducted using the training dataset. Results: The GEO database provided GSE12452 and GSE13597 datasets. The limma package filtered 58 differential genes, including 13 upregulated and 35 downregulated genes, which were visualized using volcano maps, heat maps, and PCA. Enrichment analysis results indicated a significant association between inflammation and disease. The Lasso and SVM algorithms were applied to screen 9 and 37 differential genes, respectively. Overlapping genes associated with nasopharyngeal cancer include ATP12A, LAMB1, RAD51AP1, CXCL11, BINSM1, PTGS2, and SAA1. Their diagnostic significance was confirmed using the operational characteristic Receptor of the validation group, and they were found to be highly connected to the degree of immune cell infiltration. Conclusions: These seven genes are considered promising biomarkers for diagnosing nasopharyngeal cancer, paving the way for future research on the immunological mechanisms and therapy of nasopharyngeal carcinoma.

show abstract

Section: Discussionmentioning

confidence: 99%

Identifying immune cell infiltration and diagnostic biomarkers for nasopharyngeal carcinoma through bioinformatic analysis

zhao,

Qiu,

2024

Preprint

View full text Add to dashboard Cite

show abstract

“…We found that Cur promotes the apoptosis of human colorectal cancer cells and inhibits the proliferation, migration, and invasion of tumor cells. Of note, a number of studies have revealed that PTGS2, AKT, and ESR1 are key proteins involved in cancer cell proliferation, migration, and invasion, 42 − 44 while p53 and its downstream genes Bcl-2 and Bax play crucial roles in the apoptosis of tumor cells. 45 − 47 Importantly, we found that Cur markedly decreases AKT phosphorylation and subsequently inhibits PTGS2 and ESR1 expression, reducing the proliferation, migration, and invasion of colorectal cancer cells.…”

Section: Discussionmentioning

confidence: 99%

Exploration of the Molecular Mechanism of Curcuma aromatica Salisb’s Anticolorectal Cancer Activity via the Integrative Approach of Network Pharmacology and Experimental Validation

Yin,

Tan,

Jiang

2024

ACS Omega

View full text Add to dashboard Cite

Curcuma aromatica Salisb (Cur), a well-known herbal medicine, has a wide spectrum of anti-inflammatory, anticarcinogenic, and antioxidant activities. However, the roles of its active compounds and potential mechanisms in colorectal cancer remain unknown. This research utilized network pharmacology and experimental validation to explore the possible mechanisms by which Cur protects against colorectal cancer. The active compounds of Cur and related genes for colorectal cancer were obtained from public databases. The DrugBank database was used to search for anticolorectal cancer drugs licensed through the FDA and their targets, and a "drug-component-target" relationship network was created using the Cytoscape program. The String database produced the PPI network. The ability of these active ingredients to bind to core targets was confirmed by molecular docking using AutoDock Vina. Cell and animal experiments were then carried out. A total of 274 targets were obtained from Cur, 49 of which were potential therapeutic targets. Four key targets, PTGS2, AKT1, TP53, and estrogen receptor 1 (ESR1), were screened via the PPI network and the FDA drug-target network. Molecular docking results revealed that Cur had strong binding abilities to these targets. In vivo and in vitro experiments demonstrated that Cur suppressed the development of colorectal cancer by regulating its targets (PTGS2, AKT1, TP53, and ESR1), which play crucial roles in promoting apoptosis and suppressing cell proliferation, migration, and invasion. Collectively, Cur protects against colorectal cancer by regulating the AKT1/PTGS2/ESR1 and P53 pathways, which lays the groundwork for further research and clinical applications of Cur in colorectal cancer therapy.

show abstract

“…Nevertheless, specific targets for the miRNAs of both miR-125b and miR-26a within these signaling pathways remain unidentified. Presently, miR-142-3p, miR-125b, and miR-26a also has been extensively investigated and confirmed as key regulators in diverse cancers (80)(81)(82), suggesting that these miRNAs play multifaceted roles in biological processes.…”

Section: Mirnas Targeting Host Factors Involved In Prrsv Replicationmentioning

confidence: 99%

Role of microRNAs in host defense against porcine reproductive and respiratory syndrome virus infection: a hidden front line

Huang,

Liu

2024

Front. Immunol.

View full text Add to dashboard Cite

Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most globally devastating viruses threatening the swine industry worldwide. Substantial advancements have been achieved in recent years towards comprehending the pathogenesis of PRRSV infection and the host response, involving both innate and adaptive immune responses. Not only a multitude of host proteins actively participate in intricate interactions with viral proteins, but microRNAs (miRNAs) also play a pivotal role in the host response to PRRSV infection. If a PRRSV–host interaction at the protein level is conceptualized as the front line of the battle between pathogens and host cells, then their fight at the RNA level resembles the hidden front line. miRNAs are endogenous small non-coding RNAs of approximately 20–25 nucleotides (nt) that primarily regulate the degradation or translation inhibition of target genes by binding to the 3’-untranslated regions (UTRs). Insights into the roles played by viral proteins and miRNAs in the host response can enhance our comprehensive understanding of the pathogenesis of PRRSV infection. The intricate interplay between viral proteins and cellular targets during PRRSV infection has been extensively explored. This review predominantly centers on the contemporary understanding of the host response to PRRSV infection at the RNA level, in particular, focusing on the twenty-six miRNAs that affect viral replication and the innate immune response.

show abstract

miR-26a-5p suppresses nasopharyngeal carcinoma progression by inhibiting PTGS2 expression

Cited by 9 publications

References 37 publications

Identifying immune cell infiltration and diagnostic biomarkers for nasopharyngeal carcinoma through bioinformatic analysis

Identifying immune cell infiltration and diagnostic biomarkers for nasopharyngeal carcinoma through bioinformatic analysis

Exploration of the Molecular Mechanism of Curcuma aromatica Salisb’s Anticolorectal Cancer Activity via the Integrative Approach of Network Pharmacology and Experimental Validation

Role of microRNAs in host defense against porcine reproductive and respiratory syndrome virus infection: a hidden front line

Contact Info

Product

Resources

About