MiR-26a-5p regulates proliferation, apoptosis, migration and invasion via inhibiting hydroxysteroid dehydrogenase like-2 in cervical cancer cell

Abstract

Background Evidences have indicated that miR-26a-5p regulates the malignant properties of various tumor cells. However, the influences of miR-26a-5p on proliferation, apoptosis and invasion are still vague in the cervical cancer (CC) cells. Methods The miRNA microarray and real-time quantitative PCR (RT-qPCR) analysis were utilized to detect the expression of miR-26a-5p in the patients with CC. Kaplan–Meier plotter was performed to evaluate the overall survival (OS) of the patients with CC. The CCK-8, flow cytometry, transwell and wound healing analyses were respectively used to analyze proliferation, migration and invasion in the CC cells. RT-qPCR, western blot and IHC analysis were executed to measure the expression of hydroxysteroid dehydrogenase like-2 (HSDL2) in the patients with CC. Bioinformatics and luciferase reporter assay were carried out to verify the relationship of miR-26a-5p and HSDL2. Results The expression of miR-26a-5p was downregulated and low expression of miR-26a-5p indicated a poor OS in patients with CC. Overexpression of miR-26a-5p significantly inhibited proliferation, migration and invasion, accelerated apoptosis in the Hela and C33A cells. The expression of HSDL2 was upregulated, and negatively correlated with miR-26a-5p in the patients with CC. HSDL2 was directly targeted by miR-26a-5p and rescue experiments displayed that HSDL2 partially abolished proliferation, apoptosis, migration, and invasion induced by miR-26a-5p in CC cells. Conclusions MiR-26a-5p alleviated progression of CC by suppressing proliferation, migration and invasion, promoting apoptosis through downregulating HSDL2.

miR‐26a‐5p alleviates CFA‐induced chronic inflammatory hyperalgesia through Wnt5a/CaMKII/NFAT signaling in mice

“…In previous studies, miR‐26a‐5p has shown a promising biological effects and therapeutic potential in various diseases. Many investigations reported the anticancer effect of miR‐26a‐5p through inducing apoptosis, inhibiting proliferation, and decreasing metastasis of different cancer cells 53–55 . Apart from cancers, miR‐26a‐5p also exhibit protection in cardiovascular diseases.…”

“…Many investigations reported the anticancer effect of miR‐26a‐5p through inducing apoptosis, inhibiting proliferation, and decreasing metastasis of different cancer cells. 53 , 54 , 55 Apart from cancers, miR‐26a‐5p also exhibit protection in cardiovascular diseases. It could protect against myocardial ischemia/reperfusion injury by regulating PTEN/PI3K/AKT signaling pathway 56 and also alleviates myocardial infarction by regulating ATG12‐mediated autophagy in cardiomyocytes.…”

Selection of endogenous control and identification of significant microRNA deregulations in cervical cancer

“…A study by Dong et al demonstrated that miR-26a-5p is a tumor suppressor that inhibits cell proliferation and invasion of cervical cancer cells under physiological conditions by targeting protein tyrosine phosphatase type IVA1 (49). In contrast, Li et al, 2022 identified a different target (50). They demonstrated a negative correlation of miR-26a-5p with hydroxysteroid dehydrogenases like 2 and described a therapeutic potential in increasing miR-26a-5p expression.…”

Expression and Regulatory Mechanisms of MicroRNA in Cholesteatoma: A Systematic Review

“…MiR-1297 and miR-26a-5p are other miRNAs that have been studied in cholesteatoma and are widely investigated in oncology. The inhibitory effect of miR-1297 on cancer progression has been observed in various malignant entities [113][114][115][116][117][118][119][120][121][122][123][124]. In addition to its role in tumorigenesis, miR-26a-5p similarly plays an important role in pulmonary fibrosis [125] and Alzheimer's disease [126].…”