miR-24-3p and Body Mass Index as Type 2 Diabetes Risk Factors in Spanish Women 15 Years after Gestational Diabetes Mellitus Diagnosis

Blanco, Jessica Ares; Lambert, Carmen; Fernández-Sanjurjo, Manuel; Sánchez, Paula Morales; Pujante, Pedro; Pinto-Hernández, Paola; Iglesias‐Gutiérrez, Eduardo; Torre, Edelmiro Menéndez; Delgado, Elías

doi:10.3390/ijms24021152

Cited by 5 publications

(3 citation statements)

References 37 publications

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“…Gene expression data are expressed as target gene mRNA expression relative to the corresponding housekeeping mean gene expression (ΔC T = C T miRNA—geometric mean C T value of the housekeeping miRNA). The relative expression of each miRNA was reported as 2 −ΔCT 16 .…”

Section: Methodsmentioning

confidence: 99%

Circulating miRNA expression in long-standing type 1 diabetes mellitus

Morales-Sánchez,

Lambert,

Ares-Blanco

et al. 2023

Sci Rep

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Type 1 diabetes is a chronic autoimmune disease which results in inefficient regulation of glucose homeostasis and can lead to different vascular comorbidities through life. In this study we aimed to analyse the circulating miRNA expression profile of patients with type 1 diabetes, and with no other associated pathology. For this, fasting plasma was obtained from 85 subjects. Next generation sequencing analysis was firstly performed to identify miRNAs that were differentially expressed between groups (20 patients vs. 10 controls). hsa-miR-1-3p, hsa-miR-200b-3p, hsa-miR-9-5p, and hsa-miR-1200 expression was also measured by Taqman RT-PCR to validate the observed changes (34 patients vs. 21 controls). Finally, through a bioinformatic approach, the main pathways affected by the target genes of these miRNAs were studied. Among the studied miRNAs, hsa-miR-1-3p expression was found significantly increased in patients with type 1 diabetes compared to controls, and positively correlated with glycated haemoglobin levels. Additionally, by using a bioinformatic approach, we could observe that changes in hsa-miR-1-3p directly affect genes involved in vascular development and cardiovascular pathologies. Our results suggest that, circulating hsa-miR-1-3p in plasma, together with glycaemic control, could be used as prognostic biomarkers in type 1 diabetes, helping to prevent the development of vascular complications in these patients.

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Section: Methodsmentioning

confidence: 99%

Circulating miRNA expression in long-standing type 1 diabetes mellitus

Morales-Sánchez,

Lambert,

Ares-Blanco

et al. 2023

Sci Rep

Self Cite

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“…The blocking of its overexpression with exosomes from hypoxic ECs activated the AKT/MTOR/HIF-1α pathway, increasing the survival and engraftment of ASCs [103]. MiR-24-3p increased in obese and type 2 diabetes patients [104]. The downregulation of miR-24-3p in ASCs preserved ASC survival by restoring CDK4 expression and phosphorylated Rb protein levels [105].…”

Section: Aging and Metabolic Reprogramming Decrease The Number And Fu...mentioning

confidence: 99%

Aging and Metabolic Reprogramming of Adipose-Derived Stem Cells Affect Molecular Mechanisms Related to Cardiovascular Diseases

Holvoet

2023

Cells

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We performed a systematic search of the PubMed database for English-language articles related to the function of adipose-derived stem cells in the pathogenesis of cardiovascular diseases. In preclinical models, adipose-derived stem cells protected arteries and the heart from oxidative stress and inflammation and preserved angiogenesis. However, clinical trials did not reiterate successful treatments with these cells in preclinical models. The low success in patients may be due to aging and metabolic reprogramming associated with the loss of proliferation capacity and increased senescence of stem cells, loss of mitochondrial function, increased oxidative stress and inflammation, and adipogenesis with increased lipid deposition associated with the low potential to induce endothelial cell function and angiogenesis, cardiomyocyte survival, and restore heart function. Then, we identify noncoding RNAs that may be mechanistically related to these dysfunctions of human adipose-derived stem cells. In particular, a decrease in let-7, miR-17-92, miR-21, miR-145, and miR-221 led to the loss of their function with obesity, type 2 diabetes, oxidative stress, and inflammation. An increase in miR-34a, miR-486-5p, and mir-24-3p contributed to the loss of function, with a noteworthy increase in miR-34a with age. In contrast, miR-146a and miR-210 may protect stem cells. However, a systematic analysis of other noncoding RNAs in human adipose-derived stem cells is warranted. Overall, this review gives insight into modes to improve the functionality of human adipose-derived stem cells.

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“…Being overweight and advanced maternal age, as well as having a family history of dysglycemia, are established risk factors for the development of GDM. Moreover, women with GDM are known have a tenfold increase in risk of developing type 2 diabetes mellitus (T2DM) [7,8]. Furthermore, GDM causes maternal, fetal, and neonatal complications, all of which lead to diabetes.…”

Section: Introductionmentioning

confidence: 99%

Dissecting the Molecular Role of ADIPOQ SNPs in Saudi Women Diagnosed with Gestational Diabetes Mellitus

et al. 2023

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The traditional definition of gestational diabetes mellitus (GDM) is the leading cause of carbohydrate intolerance in hyperglycemia of varying severity, with onset or initial detection during pregnancy. Previous studies have reported a relationship among obesity, adiponectin (ADIPOQ), and diabetes in Saudi Arabia. ADIPOQ is an adipokine that is produced and secreted by adipose tissue involved in the regulation of carbohydrate and fatty acid metabolism. This study investigated the molecular association between rs1501299, rs17846866, and rs2241766 single-nucleotide polymorphisms (SNPs) in ADIPOQ and GDM in Saudi Arabia. Patients with GDM and control patients were selected, and serum and molecular analyses were performed. Statistical analyses were performed on clinical data, Hardy Weinberg Equilibrium, genotype and allele frequencies, multiple logistic regression, ANOVA, haplotype, linkage disequilibrium, as well as MDR and GMDR analyses. The clinical data showed significant differences in various parameters between the GDM and non-GDM groups (p < 0.05). In GDM women with alleles, genotypes, and different genetic models, the rs1501299 and rs2241766 SNPs showed a strong association (p < 0.05). Multiple logistic regression analysis revealed a negative correlation (p > 0.05). This study concluded that rs1501299 and rs2241766 SNPs were strongly associated with GDM in women in Saudi Arabia.

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miR-24-3p and Body Mass Index as Type 2 Diabetes Risk Factors in Spanish Women 15 Years after Gestational Diabetes Mellitus Diagnosis

Cited by 5 publications

References 37 publications

Circulating miRNA expression in long-standing type 1 diabetes mellitus

Circulating miRNA expression in long-standing type 1 diabetes mellitus

Aging and Metabolic Reprogramming of Adipose-Derived Stem Cells Affect Molecular Mechanisms Related to Cardiovascular Diseases

Dissecting the Molecular Role of ADIPOQ SNPs in Saudi Women Diagnosed with Gestational Diabetes Mellitus

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