miR-23c regulates wound healing by targeting stromal cell-derived factor-1α (SDF-1α/CXCL12) among patients with diabetic foot ulcer

Amin, Karan Naresh; Umapathy, Dhamodharan; Anandharaj, Arunkumar; Jayasuriya, Ravichandran; Sasikumar, Changam Sheela; Chandra, Sathish Kumar Rajappan; Kesavan, Rajesh; Ramkumar, Kunka Mohanram

doi:10.1016/j.mvr.2019.103924

Cited by 40 publications

(26 citation statements)

References 47 publications

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“…MiR-23a-3p also suppressed oxidative stress injury in a mouse model of focal cerebral ischemia-reperfusion [93]. Furthermore, miR-23c, but not miR-23a was reported to function as a new regulator to inhibit angiogenesis by targeting SDF-1α, which is responsible for delayed process of wound healing in patients with diabetic foot ulcer due to reduced expression of growth factors, persistant inflammation and endothelial dysfunction [94]. Since our recent study demonstrated upregulation of miR-23a-3p in a proportion of children with normal clinical findings born of gestational hypertension complicated pregnancies only, we suppose that compensatory effect of miR-23a-3p may appear more likely in these children to normalise cardiomyocyte state and vasculogenesis [95].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Vascular Endothelial Function in Young and Middle-Aged Women with Respect to a History of Pregnancy, Pregnancy-Related Complications, Classical Cardiovascular Risk Factors, and Epigenetics

Hromadníková

Dvořáková

Krofta

2020

IJMS

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The aim of the study was to examine the effect of previous pregnancies and classical cardiovascular risk factors on vascular endothelial function in a group of 264 young and middle-aged women 3 to 11 years postpartum. We examined microvascular functions by peripheral arterial tonometry and EndoPAT 2000 device with respect to a history of gestational hypertension, preeclampsia, fetal growth restriction, the severity of the disease with regard to the degree of clinical signs and delivery date. Besides, we compared Reactive Hyperemia Index (RHI) values and the prevalence of vascular endothelial dysfunction among the groups of women with normal and abnormal values of BMI, waist circumference, systolic and diastolic blood pressures, heart rate, total serum cholesterol levels, serum high-density lipoprotein cholesterol levels, serum low-density lipoprotein cholesterol levels, serum triglycerides levels, serum lipoprotein A levels, serum C-reactive protein levels, serum uric acid levels, and plasma homocysteine levels. Furthermore, we determined the effect of total number of pregnancies and total parity per woman, infertility and blood pressure treatment, presence of trombophilic gene mutations, current smoking of cigarettes, and current hormonal contraceptive use on the vascular endothelial function. We also examined the association between the vascular endothelial function and postpartum whole peripheral blood expression of microRNAs involved in pathogenesis of cardiovascular/cerebrovascular diseases (miR-1-3p, miR-16-5p, miR-17-5p, miR-20a-5p, miR-20b-5p, miR-21-5p, miR-23a-3p, miR-24-3p, miR-26a-5p, miR-29a-3p, miR-92a-3p, miR-100-5p, miR-103a-3p, miR-125b-5p, miR-126-3p, miR-130b-3p, miR-133a-3p, miR-143-3p, miR-145-5p, miR-146a-5p, miR-155-5p, miR-181a-5p, miR-195-5p, miR-199a-5p, miR-210-3p, miR-221-3p, miR-342-3p, miR-499a-5p, and miR-574-3p). A proportion of overweight women (17.94% and 20.59%) and women with central obesity (18.64% and 21.19%) had significantly lower RHI values at 10.0% false positive rate (FPR) both before and after adjustment of the data for the age of patients. At 10.0% FPR, a proportion of women with vascular endothelial dysfunction (RHI ≤ 1.67) was identified to have up-regulated expression profile of miR-1-3p (11.76%), miR-23a-3p (17.65%), and miR-499a-5p (18.82%) in whole peripheral blood. RHI values also negatively correlated with expression of miR-1-3p, miR-23a-3p, and miR-499a-5p in whole peripheral blood. Otherwise, no significant impact of other studied factors on vascular endothelial function was found. We suppose that screening of these particular microRNAs associated with vascular endothelial dysfunction may help to stratify a highly risky group of young and middle-aged women that would benefit from early implementation of primary prevention strategies. Nevertheless, it is obvious, that vascular endothelial dysfunction is just one out of multiple cardiovascular risk factors which has only a partial impact on abnormal expression of cardiovascular and cerebrovascular disease associated microRNAs in whole peripheral blood of young and middle-aged women.

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Section: Discussionmentioning

confidence: 99%

Evaluation of Vascular Endothelial Function in Young and Middle-Aged Women with Respect to a History of Pregnancy, Pregnancy-Related Complications, Classical Cardiovascular Risk Factors, and Epigenetics

Hromadníková

Dvořáková

Krofta

2020

IJMS

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“…qPCR reaction mixture (20 µL) contained 10 µL fast start essential SYBR Green PCR Master Mix (Roche Diagnostics, GmbH, Indianapolis, IN, USA), 2 µL (0.2µM) of each T. castaneum specific forward and reverse primers, and 5 µL of DNA corresponding to 100 ng/reaction served as a template for the study. The amplification conditions were followed as described by Amin et al ( 31 ). Samples without template DNA were used as negative control and the DNA isolated from the adult red flour beetle was used as a positive control.…”

Section: Methodsmentioning

confidence: 99%

A Molecular Approach for the Detection and Quantification of Tribolium castaneum (Herbst) Infestation in Stored Wheat Flour

Negi

Anandharaj

Kalakandan

et al. 2021

Food Technol. Biotechnol. (Online)

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Research background. The presence of insect fragments is one of the major constrains in stored food commodities and cause considerable loss in the quality of the produce. The management of the pest is viewed as a huge challenge in food processing industry. Conventionally, the detection of T. castenaum in the food processing industry is carried out by acid hydrolysis and staining methods which are not precise and time consuming. Experimental approach. Considering the importance of a quick and effective method, a qPCR-based approach was developed and elucidated in this study. The mitochondrial cytochrome oxidase I (mtCOI) gene was identified as a target due to the abundance in the pest. Specific primers were designed against the target gene by primer premier software and amplified in a qPCR. Results and conclusions. This developed method is capable of detecting all the ontogenic stages of T. castaneum in stored wheat flour. Earlier experiments had demonstrated that about 20 µg of DNA can be obtained from 2.2 mg of insects, to quantify the infestation levels the Ct values obtained from known samples were subjected to regression analysis and expressed as adult equivalents. In the unknown samples tested, the infestation was calculated as 1.74 and 0.046 adult insects in 5 g of wheat flour. The maximum permissible limit of insect fragments in flour is 75 insect fragments or approximately 3 adults per 50 g of flour as per FDA. Hence, by adopting this new method, it is possible for the warehouse operators to arrive at a decision to proceed with efficient management practices were wheat flour is stored. Also, this method can be ratified by government agencies associated with international business to ascertain whether the wheat flour meets the standards set by the respective country before subjecting to foreign trade. Novelty and scientific contribution. This study is the first of its kind in the detection and quantification for T. castaneum in milled products. So far only conventional methods are employed to assess the presence of the pest and manual counting of fragments are practiced to quantify the infestation levels. The developed qPCR method is faster, reliable and can be employed in milling industries, Bakery industries, food processing plants and foreign trade units for critical detection and quantification T. castaneum pest infestation.

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“…miR-23c is also involved in the downregulation of the expression of other angiogenic factors, such as endothelial NOS (eNOS), HIF-1a, and VEGF, thus inhibiting angiogenesis. 84 miR-205-5p inhibits VEGF protein translation by interacting with the 3 0 UTR of VEGF mRNA. In contrast, anti-miR-205-5p significantly increased the expression of VEGF in MSCs, thus promoting angiogenesis.…”

Section: Anti-angiogenesismentioning

confidence: 99%

“…16,30,48,75,121 A disease may be accompanied by several changes in the expression of different ncRNAs, 111,112,122 and their functions differ markedly. 84 The expression dynamics and individual differences of ncRNAs as well as their mutual relationship are like a complex network, which greatly increases the challenge of clinical application of ncRNAs. Use of ncRNAs as a diagnostic biomarker is based on changes in their level of expression or restoration of expression through artificial supplementation for treatment purposes.…”

Section: Challenges In the Application Of Ncrnasmentioning

confidence: 99%

The progress, prospects, and challenges of the use of non-coding RNA for diabetic wounds

Wei

Liu

et al. 2021

Molecular Therapy - Nucleic Acids

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Chronic diabetic wounds affect the quality of life of patients, resulting in significant social and economic burdens on both individuals and the health care system. Although treatment methods for chronic diabetic wounds have been explored, there remains a lack of effective treatment strategies; therefore, alternative strategies must be explored. Recently, the abnormal expression of non-coding RNA in diabetic wounds has received widespread attention since it is an important factor in the development of diabetic wounds. This article reviews the regulatory role of three common non-coding RNAs (microRNA [miRNA], long non-coding RNA [lncRNA], and circular RNA [circRNA]) in diabetic wounds and discusses the diagnosis, treatment potential, and challenges of non-coding RNA in diabetic wounds. This article provides insights into new strategies for diabetic wound diagnosis and treatment at the genetic and molecular levels. ncRNA ncRNA refers to a type of RNA that does not encode protein. In the past, it has been shown to participate in a variety of biological processes and play important transcriptional and post-transcriptional regulatory functions. 18 ncRNAs are divided into two categories based on the length of the RNA chain; lncRNA have lengths greater than 200 nucleotides, and small ncRNAs (snRNA) have lengths less than 200 nucleotides. snRNAs can be subdivided into miRNAs and circRNAs. 19 miRNAs inhibit the expression of target genes by binding to the 3 0 untranslated region (3 0 UTR) of the target mRNA. 20 The miRNA gene is processed and cleaved by RNA polymerase II (Pol II) enzyme and endonuclease Drosha III to produce pre-miRNA, which is

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miR-23c regulates wound healing by targeting stromal cell-derived factor-1α (SDF-1α/CXCL12) among patients with diabetic foot ulcer

Cited by 40 publications

References 47 publications

Evaluation of Vascular Endothelial Function in Young and Middle-Aged Women with Respect to a History of Pregnancy, Pregnancy-Related Complications, Classical Cardiovascular Risk Factors, and Epigenetics

Evaluation of Vascular Endothelial Function in Young and Middle-Aged Women with Respect to a History of Pregnancy, Pregnancy-Related Complications, Classical Cardiovascular Risk Factors, and Epigenetics

A Molecular Approach for the Detection and Quantification of Tribolium castaneum (Herbst) Infestation in Stored Wheat Flour

The progress, prospects, and challenges of the use of non-coding RNA for diabetic wounds

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