miR‐23b‐3p regulates apoptosis and autophagy via suppressing SIRT1 in lens epithelial cells

Abstract

Age-related cataract is one of the prior causes of blindness and the incidence rates of cataract are even rising. Oxidative stress plays an important role in the pathogenesis of cataracts. Under oxidative stress, lens epithelial cell (LEC cell) apoptosis is activated, which might lead to the opacity of the lens and accelerate the progression of cataract development. Meanwhile, autophagy is also active to face oxidative stress. miRNAs have been reported to involve cataract. However, the underlying mechanism is not clear. The present study aimed to investigate the regulatory effect of miR23b-3p on apoptosis and autophagy in LEC cells under oxidative stress. The expression levels of miR-23b-3p were examined in age-related cataract tissues and LEC cells treated with hydrogen peroxide, showing that miR23b-3p expression levels were upregulated. Knockdown of miR23b-3p expression in LEC cells brought about apoptosis significantly decreased while autophagy significantly increased during hydrogen peroxide.We predicted microRNA miRNA-23b-3p might participate in regulating silent information regulator 1 (SIRT1) by bioinformatics database of TargetScan. Luciferase reporter assays confirmed that miRNA-23b-p could suppress SIRT1 expression by binding its 3′UTR. In addition, overexpression or knockdown of miR-23b-3p could decrease or increase SIRT1 expression, which indicated that Mir-23b-3p could suppress SIRT1 expression. In addition, enhanced SIRT1 could attenuate the regulation of cell apoptosis and autophagy induced by overexpression of miR-23b-3p. Taken together, our findings revealed that miR-23b-3p regulated apoptosis and autophagy via suppressing SIRT1 in LEC cell under oxidative stress, which could provide new ideas for clinical treatment of cataract. K E Y W O R D Sapoptosis, autophagy, cataract, miR-23b-p, SIRT1

Tumor Necrosis Factor-alpha (TNF-α) Enhances miR-155-Mediated Endothelial Senescence by Targeting Sirtuin1 (SIRT1)

“…Further evidence has suggested that the SIRT1 3′ UTR is critical for SIRT1 mRNA stability. Additionally, a series of miRNAs, including miR-132-3p, miR-23b-3p, and miR-34a, were found to modulate SIRT1 expression [9,14,15]. Our results demonstrated that miR-155 upregulation inhibited SIRT1 expression and HUVEC proliferation and promoted TNF-α-induced senescence in HUVECs.…”

“…Previous reports have shown that the SIRT1 3′-untranslated region (3′ UTR) is critical for its mRNA stability. Additionally, a series of microRNAs (miRNAs), such as miR-34a, miR-23b-3p, and miR-132-3p, modulate SIRT1 expression [9,14,15].…”

The Encystment-Related MicroRNAs and Its Regulation Molecular Mechanism in Pseudourostyla cristata Revealed by High Throughput Small RNA Sequencing

“…The previous morphological study found that, during encystment of P. cristata, cytoplasmic structures such as extrusomes were wrapped in membrane vesicles to form autophagic vesicles or autophagosomes in the intracellular self-digestion phenomenon [15]. Scientists found that knockdown of miR-23b-3p expression in LEC cells elevated autophagy significantly during hydrogen peroxide stress [16]. We found that miR-23b-3p was downregulated during the cyst formation.…”

Evaluation of microRNA profile in cervical epithelium for predicting cervical cancer recurrence

“…МикроРНК-23b, в отличие от вышеописанных микроРНК-20а и -21, является опухолевым супрессором, ингибируя посредством влияния на гены-мишени SRC и AKT1 клеточную пролиферацию, миграцию и инвазию, а также индуцируя остановку клеточного цикла в стадии G0/G1 [25]. Наши данные согласуются с представлением других исследователей о роли этого онкомаркера.…”