MiR‐222 inhibits apoptosis in porcine follicular granulosa cells by targeting the <i>THBS1</i> gene

Zhu, Weihua; Yang, Min; Shang, Jinnan; Xu, Yifan; Wang, Yuanlang; Tao, Qiangqiang; Zhang, Liang; Ding, Yueyun; Chen, Yige; Zhao, Dongdong; Wang, Chonglong; Chu, Mingxing; Yin, Zhan; Zhang, Xiaodong

doi:10.1111/asj.13208

Cited by 20 publications

(11 citation statements)

References 46 publications

(58 reference statements)

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“…A previous study reported that THBS-1 inhibits angiogenesis and induces endothelial cell apoptosis [33]. Zhu et al demonstrated that miR-222 inhibits apoptosis in porcine follicular granulosa cells by suppressing the expression of THBS1 [34]. Based on previous studies and the current results, we postulate that cfa-let-7a suppresses cellular apoptosis by targeting THBS1, which would be beneficial for ORFV replication in GSF cells.…”

Section: Discussionsupporting

confidence: 71%

“…Overall, THBS1 was downregulated much more than other four cfa-let-7a targets based on the above results. Furthermore, previous studies reported that THBS1 was related to apoptosis [33,34] which was an important immune mechanism used by cells against viral infection. Therefore, we first selected THBS1 for Western blot analysis.…”

Section: Cfa-let-7a_r+2 Target Prediction and Validationmentioning

confidence: 99%

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Integrated Analysis of Differentially Expressed miRNAs and mRNAs in Goat Skin Fibroblast Cells in Response to Orf Virus Infection Reveals That cfa-let-7a Regulates Thrombospondin 1 Expression

Pang

Wang

Chen

et al. 2020

Viruses

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Orf is a zoonotic disease that has caused huge economic losses globally. Systematical analysis of dysregulated cellular micro RNAs (miRNAs) in response to Orf virus (ORFV) infection has not been reported. In the current study, miRNA sequencing and RNA sequencing (RNA-seq) were performed in goat skin fibroblast (GSF) cells at 0, 18, and 30 h post infection (h.p.i). We identified 140 and 221 differentially expressed (DE) miRNAs at 18 and 30 h.p.i, respectively. We also identified 729 and 3961 DE genes (DEGs) at 18 and 30 h.p.i, respectively. GO enrichment analysis indicates enrichment of apoptotic regulation, defense response to virus, immune response, and inflammatory response at both time points. DE miRNAs and DEGs with reverse expression were used to construct miRNA-gene networks. Seven DE miRNAs and seven DEGs related to “negative regulation of viral genome replication” were identified. These were validated by RT-qPCR. Cfa-let-7a, a significantly upregulated miRNA, was found to repress Thrombospondin 1 (THBS1) mRNA and protein expression by directly targeting the THBS1 3′ untranslated region. THBS1 has been reported to induce apoptosis; therefore, the cfa-let-7a-THBS1 axis may play an important role in cellular apoptosis during ORFV infection. This study provides new insights into ORFV and host cell interaction mechanisms.

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Section: Discussionsupporting

confidence: 71%

Section: Cfa-let-7a_r+2 Target Prediction and Validationmentioning

confidence: 99%

Integrated Analysis of Differentially Expressed miRNAs and mRNAs in Goat Skin Fibroblast Cells in Response to Orf Virus Infection Reveals That cfa-let-7a Regulates Thrombospondin 1 Expression

Pang

Wang

Chen

et al. 2020

Viruses

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“…The final two granulosa subclusters can be identified as mitotic and atretic cells based on their expression of previously validated markers. Indeed, transcripts enriched in the latter are consistent with markers of atretic follicles including Caldesmon 1 (Cald1) (Hatzirodos et al 2014), Phosphoinositide-3-Kinase Interacting Protein 1(Pik3ip1) (Piersanti et al 2019), Thrombospondin 1 (Thbs1) (Thomas et al 2008; Garside et al 2010; Zhu et al 2019), and SRY-Box Transcription Factor 4 (Sox4) (Terenina et al 2017; Fan et al 2019). While markers found in mitotic granulosa cells are universally characteristic of dividing cells ( Table S5 ).…”

Section: Discussionmentioning

confidence: 90%

A single cell atlas of the cycling murine ovary

Morris

Meinsohn

Chauvin

et al. 2022

Preprint

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The estrous cycle is regulated by rhythmic endocrine interactions of the nervous and reproductive systems, which coordinate the hormonal and ovulatory functions of the ovary. Folliculogenesis and follicle progression require the orchestrated response of a variety of cell types to allow the maturation of the follicle and its sequela, ovulation, corpus luteum (CL) formation, and ovulatory wound repair. Little is known about the cell state dynamics of the ovary during the estrous cycle, and the paracrine factors that help coordinate this process. Herein we used single-cell RNA sequencing technology to evaluate the transcriptome of over 34,000 cells of the adult mouse ovary and describe the transcriptional changes that occur across the normal estrous cycle and other reproductive states to build a comprehensive dynamic atlas of murine ovarian cell types and states.

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“…Higher expressions of CASP3 , one of the pro-apoptotic genes, induce the apoptosis of GCs in bovines [36]. Downregulation of the pro-apoptotic CASP3 gene inhibits the apoptosis of GCs in pigs [40]. The mRNA level of CASP3 in GCs is negatively associated with the number of preovulatory follicles in humans, indicating that the accumulation of CASP3 impairs the development of follicles [41].…”

Section: Discussionmentioning

confidence: 99%

P65 Targets FGFR1 to Regulate the Survival of Ovarian Granulosa Cells

Yuan

Kong

et al. 2019

Cells

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In female mammals, the abnormal apoptosis of ovarian granulosa cells (GCs) impairs follicular development and causes reproductive dysfunction. Many studies have indicated that the FGFR1 gene of the PI3K signaling pathway and the p65 subunit of the transcription factor NF-κB may regulate the proliferation and apoptosis of GCs involved in follicular development. However, little is known about whether p65 regulates the transcription of FGFR1, as well as the biological effects of p65 and FGFR1 on the survival of GCs and follicular development. In porcine follicles and GCs, we found that p65 and FGFR1 were exclusively expressed in the GCs of follicles, and the mRNA and protein levels of p65 and FGFR1 significantly increased from small to large follicles. Both p65 and FGFR1 were found to activate the PI3K signaling pathway, and the expressions of proliferation markers (PCNA and MKI67) and the anti-apoptotic gene BCL2 were significantly increased by p65 and FGFR1. Furthermore, both p65 and FGFR1 were observed to promote cell proliferation and inhibit the cell apoptosis of GCs, and p65 was confirmed to bind at the −348/−338 region of FGFR1 to positively regulate its transcription. Moreover, p65 was further found to enhance the pro-proliferation and anti-apoptotic effects of FGFR1. Taken together, p65 may target the −348/−338 region of FGFR1, promote the transcription of FGFR1, and enhance the pro-proliferation effect and anti-apoptotic effect of FGFR1 to facilitate the growth of follicles. This study will provide useful information for further investigations on the p65-mediated-FGFR1 signaling pathway during folliculogenesis in mammals.

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MiR‐222 inhibits apoptosis in porcine follicular granulosa cells by targeting the THBS1 gene

Cited by 20 publications

References 46 publications

Integrated Analysis of Differentially Expressed miRNAs and mRNAs in Goat Skin Fibroblast Cells in Response to Orf Virus Infection Reveals That cfa-let-7a Regulates Thrombospondin 1 Expression

Integrated Analysis of Differentially Expressed miRNAs and mRNAs in Goat Skin Fibroblast Cells in Response to Orf Virus Infection Reveals That cfa-let-7a Regulates Thrombospondin 1 Expression

A single cell atlas of the cycling murine ovary

P65 Targets FGFR1 to Regulate the Survival of Ovarian Granulosa Cells

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