MiR-219a-5p enhances cisplatin sensitivity of human non-small cell lung cancer by targeting FGF9

Rao, Chuangzhou; Miao, Xiaobo; Zhao, Guofang; Zhang, Chen; Shen, Huan; Dong, Congcong; Yang, Minglei

doi:10.1016/j.biopha.2019.108662

Cited by 24 publications

(23 citation statements)

References 28 publications

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“…In the present study, we confirmed circCDR1as as a sponge of miR‐219a‐5p by dual‐luciferase reporter assay. Moreover, we found that miR‐219a‐5p expression declined in NSCLC and its overexpression repressed NSCLC development in vitro, which was also in agreement with previous studies . This indicated the anti‐cancer role of miR‐219a‐5p in NSCLC.…”

Section: Discussionsupporting

confidence: 92%

“…To further elucidate the ceRNA network in this study, the targets of miR‐219a‐5p were analyzed. Previous studies have confirmed multiple targets of miR‐219a‐5p in different conditions, such as high mobility group protein A2 (HMGA2), fibroblast growth factor 9 (FGF9) and eyes absent homolog 2 (EYA2) . In this study, we first confirmed the target association between miR‐219a‐5p and SOX5 in NSCLC cells.…”

Section: Discussionsupporting

confidence: 74%

“…Moreover, we found that miR-219a-5p expression declined in NSCLC and its overexpression repressed NSCLC development in vitro, which was also in agreement with previous studies. 18,19 This indicated the anti-cancer role of miR-219a-5p in NSCLC. In addition, our research data revealed that the suppressive effect of circCDR1as knockdown on NSCLC development was attenuated by miR-219a-5p inhibitor, revealing the possibility that circCDR1as might contribute to the development of NSCLC by sponging miR-219a-5p.…”

Section: Discussionmentioning

confidence: 92%

“…MicroRNAs (miRNAs) are associated with the development, detection and treatment of NSCLC . Previous studies suggest that low expression of miR‐219a‐5p is required for the development of NSCLC . Moreover, the sex determining region Y‐box protein (SOX) family is crucial for multiple processes in the progression of human cancers .…”

Section: Introductionmentioning

confidence: 99%

“…17 Previous studies suggest that low expression of miR-219a-5p is required for the development of NSCLC. 18,19 Moreover, the sex determining region Y-box protein (SOX) family is crucial for multiple processes in the progression of human cancers. 20 SOX5 is a representative of the SOX family which could drive the malignancy of lung cancer, including NSCLC.…”

Section: Introductionmentioning

confidence: 99%

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CircRNA CDR1as knockdown inhibits progression of non‐small‐cell lung cancer by regulating miR‐219a‐5p/SOX5 axis

Zhang

Pan

et al. 2020

Thoracic Cancer

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Background Circular RNAs (circRNAs) participate in the development of human cancers by regulating multiple cell processes. CircRNA antisense to the cerebellar degeneration‐related protein 1 transcript (circCDR1as) expression is dysregulated in many cancers, including non‐small‐cell lung cancer (NSCLC). However, the mechanism by which circCDR1as mediates the development of NSCLC remains unknown. Methods A total of 30 paired cancer and normal tissues were collected from patients with NSCLC. The expression levels of circCDR1as, microRNA (miR)‐219a‐5p and Sex determining region Y‐box protein 5 (SOX5) were measured in tissues or cells by quantitative real‐time polymerase chain reaction or western blot. Cell viability, apoptosis, migration and invasion were detected by 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl‐tetrazolium bromide, colony formation, flow cytometry and transwell assays, respectively. The target relationship between miR‐219a‐5p and circCDR1as or SOX5 was validated by dual‐luciferase reporter assay. Results CircCDR1as expression was elevated in NSCLC tissues and cells in comparison to the matched controls. Interference of circCDR1as led to obvious inhibition of cell viability, migration and invasion and increase of apoptosis in NSCLC cells. MiR‐219a‐5p acted as a target of circCDR1as and miR‐219a‐5p downregulation attenuated the regulatory effect of circCDR1as silencing on NSCLC progression. Moreover, miR‐219a‐5p targeted SOX5 to repress the progression of NSCLC in vitro. Besides, circCDR1as knockdown reduced the expression of SOX5 by increasing miR‐219a‐5p level. Conclusion Knockdown of circCDR1as inhibited the progression of NSCLC by decreasing cell viability, migration and invasion and increasing apoptosis by upregulating miR‐219a‐5p and downregulating SOX5.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 74%

Section: Discussionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%