miR-219a-1 inhibits colon cancer cells proliferation and invasion by targeting MEMO1

Abstract

Colon cancer is the third most common cancer worldwide. Many miRNAs have been reported to be involved in colon cancer progression. However, there are only a few studies on the role of miR-219a-1 in colon cancer, and the molecular mechanisms involved remain unclear. The aim of this study was to investigate the miR-219a-1 level in patients with colon cancer and to explore both the effects and regulatory mechanisms of miR-219a-1 in the malignancy of colon cancer cells. Real-time PCR and western blot analysis were used to analyze the expression levels of miR-219a-1 and mediator of ErbB2-driven cell motility 1. Cell Counting Kit-8, transwell and wound-healing assays were performed to investigate the malignant ability of colon cancer cells. A luciferase assay was performed to explore whether miR-219a-1 could directly bind to 3ʹ-UTR region of MEMO1. miR-219a-1 was found to be downregulated in colon cancer cell lines and in patients with colon cancer. Additionally, miR-219a-1 could inhibit colon cancer cell proliferation, invasion and migration. We identified MEMO1 as a novel potential target gene of miR-219a-1. Luciferase assays showed that miR-219a-1 could directly bind to 3′-UTR of MEMO1. Overexpression of miR-219a-1 in colon cancer cells could inhibit the expression of MEMO1. Furthermore, MEMO1 was upregulated in patients with colon cancer, which was inversely correlated with miR-219a-1 levels. In conclusion, our study revealed that miR-219a-1 exerts anti-tumor effects and regulates colon cancer cell proliferation, invasion and migration by targeting MEMO1, suggesting that miR-219a-1 could act as a therapeutic target in colon cancer.

Circ_0055625 knockdown inhibits tumorigenesis and improves radiosensitivity by regulating miR-338-3p/MSI1 axis in colon cancer

“…The progression of colon cancers involved lots of miRNAs, such as miR-141 [ 35 ], miR-219a-1 [ 36 ], and miR-503-5p [ 37 ]. MiR-338-3p, a miRNA, was found to play vital parts in colon cancer cell processes.…”

LncRNA cancer susceptibility 20 regulates the metastasis of human gastric cancer cells via the miR-143-5p/MEMO1 molecular axis

“…MEMO1 plays an important role in breast cancer cell invasion and migration by activating the PI3K/Akt signaling pathway, leading to up-regulated Snail1 and induction of the EMT program[ 21 ]. MEMO1 is highly expressed in colon cancer tissues and cells, and miR-219a-1 regulates the proliferation, invasion and metastasis of colon cancer cells by targeting MEMO1, thereby functioning as a tumor suppressor gene[ 22 ]. The role of MEMO1 in the progression of GC has not yet been reported.…”

Genetic Polymorphisms in microRNA Genes Targeting PI3K/Akt Signal Pathway Modulate Cervical Cancer Susceptibility in a Chinese Population

“…Many studies have reported that miRNAs regulate components of the PI3K/Akt signalling pathway ( Rahmani et al, 2020a ; Rahmani et al, 2020b ), and abnormal expression of these miRNAs might induce an out-of-control expression of their targets, which leads to disorders of the corresponding signalling pathway ( Akbarzadeh et al, 2021 ). Studies have observed the abnormal expression of miR-219a ( Xu et al, 2020 ), let-7i ( Chhabra, 2018 ), miR-149 ( Zhou and Xu, 2021 ), miR-143 ( Tang et al, 2021 ), miR-132 ( Zhang et al, 2021 ), miR-10b ( Zou et al, 2016 ), let-7a ( Wu et al, 2016 ) and miR-26a ( Dong et al, 2014 ) in CC or other human cancers, which indicated the important roles of these miRNAs in human cancers. Single nucleotide polymorphisms (SNPs) in miRNA genes can modify the expression of mature miRNAs ( Slezak-Prochazka et al, 2010 ; Króliczewski et al, 2018 ).…”