miR-218 promoted the apoptosis of human ovarian carcinoma cells via suppression of the WNT/β-catenin signaling pathway

Huang, Yan; Liang, Shanhui; Xiang, Libing; Han, Xiaotian; Zhang, W.; Tang, Jia; Wu, Xiaohua; Zhang, M. Q.

doi:10.1134/s0026893317030062

Cited by 3 publications

(2 citation statements)

References 31 publications

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“…In addition, it was reported that the Wnt/β-catenin signaling pathway was highly activated in various types of cancer, such as ovarian epithelial cancer and prostate cancer ( 51 , 52 ), thus, the inhibition of this signaling pathway has become a research hotspot. A previous study revealed that the inhibition of the Wnt/β-catenin signaling pathway inhibited growth and promoted apoptosis in ovarian cancer cells ( 53 ), while microRNA-218 promoted the apoptosis of ovarian cancer cells by inhibiting the Wnt/β-catenin signaling pathway ( 53 ). In addition, lysyl oxidase homolog 2, a member of the lysyl oxidase family, was suggested to affect the growth of ovarian cancer cells by inhibiting the Wnt/β-catenin signaling pathway ( 54 ).…”

Section: Discussionmentioning

confidence: 99%

FOXO3a inhibits nephroblastoma cell proliferation, migration and invasion, and induces apoptosis through downregulating the Wnt/β‑catenin signaling pathway

Qian

Liu

2021

Mol Med Rep

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Forkhead transcription factor O subfamily 3A (FOXO3a) is an important tumor suppressor gene that is expressed in renal tissue and has been reported to be downregulated in clear cell renal cell carcinoma (CCRCC). Notably, the overexpression of FOXO3a was previously discovered to inhibit the progression of CCRCC. However, the expression levels of FOXO3a in nephroblastoma cell lines remain unknown. The present study aimed to investigate the expression levels of FOXO3a in nephroblastoma cell lines and to determine the mechanism of action of the biological functions of FOXO3a. Western blotting and reverse transcription-quantitative PCR were used to analyze the expression levels of FOXO3a in nephroblastoma cell lines. Subsequently, the effects of the overexpression of FOXO3a and the genetic knockdown of the Wnt/β-catenin signaling protein Axin-2 on the biological functions were determined through Cell Counting Kit-8, cell colony formation assays, scratch and Transwell assay and flow cytometric analysis experiments. The expression levels of FOXO3a were discovered to be downregulated in nephroblastoma cell lines. The overexpression of FOXO3a inhibited the proliferation, invasion and migration of nephroblastoma cells, while inducing apoptosis. Furthermore, the overexpression of FOXO3a downregulated the expression levels of β-catenin and Cyclin-D1 proteins involved in the Wnt/β-catenin signaling pathway. Cell proliferation and the migration and invasion ability of 17–94 cells in shRNA-Axin2-2 group were promoted. Cell apoptosis was predominantly increased by overexpressed FOXO3a, which was reversed by shRNA-Axin2-1. The biological effects of overexpressing FOXO3a on nephroblastoma were reversed after activation of Wnt/β-catenin. In conclusion, the findings of the present study suggested that FOXO3a may inhibit nephroblastoma cell proliferation, migration and invasion, while inducing apoptosis, by downregulating the Wnt/β-catenin signaling pathway. These results may provide a novel method for the early diagnosis and precise treatment of nephroblastoma.

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Section: Discussionmentioning

confidence: 99%

FOXO3a inhibits nephroblastoma cell proliferation, migration and invasion, and induces apoptosis through downregulating the Wnt/β‑catenin signaling pathway

Qian

Liu

2021

Mol Med Rep

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“…Overexpression of miR-218 suppressed CC cell viability, migration, and invasion [72,73]. The expression of miR-218 was downregulated in human ovarian carcinoma OVCAR3 cells compared to normal ovarian cells [74]. It was observed that this miRNA inhibited cell proliferation but promoted apoptosis in OVCAR3 cells through suppression of the Wnt/β-catenin signaling pathway.…”

Section: Discussionmentioning

confidence: 98%

Differential microRNA Expression Analysis in Patients with HPV-Infected Ovarian Neoplasms

Jarych,

Mikulski,

Wilczyński

et al. 2024

IJMS

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This study aimed to identify microRNAs (miRNAs) whose expression levels are altered by high-risk human papillomavirus (HR-HPV) infection in women with epithelial ovarian neoplasms. MiRNA expression was quantified by real-time polymerase chain reaction, while HR-HPV DNA was quantified using digital-droplet PCR. Analysis of 11 miRNAs demonstrated significantly lower hsa-miR-25-5p expression in HPV-infected compared to uninfected ovarian tissues (p = 0.0405), while differences in miRNA expression in corresponding serum were statistically insignificant. The expression of hsa-miR-218-5p in ovarian tumors was significantly higher in high-grade serous ovarian carcinoma (HGSOC) cases than in other neoplasms (p = 0.0166). In addition, hsa-miR-218-5p was significantly upregulated, whereas hsa-miR-191-5p was significantly downregulated in tissues with stage III/IV FIGO (p = 0.0009 and p = 0.0305, respectively). Using unsupervised clustering, we identified three unique patient groups with significantly varied frequencies of HPV16/18-positive samples and varied miRNA expression profiles. In multivariate analysis, high expression of hsa-miR-16-5p was an independent prognostic factor for poor overall survival (p = 0.0068). This preliminary analysis showed the changes in miRNA expression in ovarian neoplasms during HPV infection and those collected from HGSOCs or patients with advanced disease. This prospective study can provide new insights into the pathogenesis of ovarian neoplasms and host–virus interactions.

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Elucidating the crosstalk between endothelial-to-mesenchymal transition (EndoMT) and endothelial autophagy in the pathogenesis of atherosclerosis

Singh,

Cui,

Eisa-Beygi

et al. 2024

Vascular Pharmacology

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miR-218 promoted the apoptosis of human ovarian carcinoma cells via suppression of the WNT/β-catenin signaling pathway

Cited by 3 publications

References 31 publications

FOXO3a inhibits nephroblastoma cell proliferation, migration and invasion, and induces apoptosis through downregulating the Wnt/β‑catenin signaling pathway

FOXO3a inhibits nephroblastoma cell proliferation, migration and invasion, and induces apoptosis through downregulating the Wnt/β‑catenin signaling pathway

Differential microRNA Expression Analysis in Patients with HPV-Infected Ovarian Neoplasms

Elucidating the crosstalk between endothelial-to-mesenchymal transition (EndoMT) and endothelial autophagy in the pathogenesis of atherosclerosis

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