miR-218 inhibited tumor angiogenesis by targeting ROBO1 in gastric cancer

Zhang, Xiangyuan; Dong, Jiaqiang; He, Yan; Zhao, Ming; Liu, Zhen; Wang, Na; Jiang, Mingzuo; Zhang, Zhe; Liu, Gang; Liu, Haiming; Nie, Yongzhan; Fan, Daiming; Tie, Jun

doi:10.1016/j.gene.2017.03.022

Cited by 48 publications

(34 citation statements)

References 32 publications

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“…The current study also identified that the expression of miR-218 was able to inhibit the high glucose-induced HUVEc proliferation, migration and angiogenesis by targeting ROBO1 and HO-1. A previous study verified that the high expression levels of ROBO1 and HO-1 were able to significantly increase cell migration, proliferation and angiogenesis (17,18,39). Herein, it was also observed that the expression of VEGF was increased in high glucose conditions.…”

Section: Discussionsupporting

confidence: 61%

“…Yang et al (17) identified that miR-218 was upregulated in podocytes treated with a high glucose concentration and promoted high glucose-induced apoptosis in these cells by targeting heme oxygenase-1 (HO-1). Zhang et al (18) also observed that miR-218 expression promoted angiogenesis by targeting roundabout 1 (ROBO1). ROBO1, also known as protein SAX3, is the receptor of slit guidance ligand 1 (SLIT1) and SLIT2, which are associated with cell migration.…”

Section: Human Circular Rna-0054633 Regulates High Glucose-induced Vamentioning

confidence: 98%

“…Previous studies have demonstrated that miR-218 targets HO-1 and ROBO1, and that the expression of miR-218 promotes high glucose-induced cell apoptosis (17,18). Furthermore, the expression of HO-1 or ROBO1 promotes cell survival.…”

Section: Interaction Of Mir-218 Ho-1 and Robo1mentioning

confidence: 98%

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Human circular RNA‑0054633 regulates high glucose‑induced vascular endothelial cell dysfunction through the microRNA‑218/roundabout 1 and microRNA‑218/heme oxygenase‑1 axes

et al. 2018

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The aim of the present study was to investigate the relative regulation of human circular RNA‑0054633 (hsa_circ_0054633), microRNA‑218 (miR‑218), roundabout 1 (ROBO1) and heme oxygenase‑1 (HO‑1) in human umbilical vein endothelial cells (HUVECs) in high glucose conditions. Initially, the expression of hsa_circ_0054633 in HUVECs was detected in high glucose conditions by reverse transcription‑quantitative polymerase chain reaction. Next, a small interfering RNA against hsa_circ_0054633 was constructed to investigate the function of jsa_circ_0054633 in HUVECs by transwell migration, cell counting kit‑8, flow cytometry and tube formation assays. In addition, the effect of hsa_circ_0054633 on the expression levels of ROBO1, HO‑1 and vascular endothelial growth factor were examined. The regulation effects of hsa_circ_0054633 on high glucose‑induced HUVEC proliferation, migration, and angiopoiesis were also analyzed. Bioinformatics analysis and dual‑luciferase assay were then used to confirm the direct or specific regulation of hsa_circ_0054633, miR‑218, ROBO1 and HO‑1. It was observed that high glucose levels increased the expression of hsa_circ_0054633, while downregulation of hsa_circRNA‑0054633 increased the high glucose‑induced endothelial cell dysfunction, including proliferation, migration and angiopoiesis suppression. Bioinformatics analysis revealed that the expression of circRNA‑0054633 was able to inhibit miR‑218 expression, which was clarified by the dual‑luciferase assay. It was also demonstrated that downregulating the expression of miR‑218 inhibited the high glucose‑induced endothelial cell dysfunction by promoting the expression of ROBO1 and HO‑1. These results suggest that the expression of hsa_circRNA‑0054633 has a protective effect against high glucose‑induced endothelial cell dysfunction by targeting ROBO1 and HO‑1.

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Section: Discussionsupporting

confidence: 61%

Section: Human Circular Rna-0054633 Regulates High Glucose-induced Vamentioning

confidence: 98%

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Human circular RNA‑0054633 regulates high glucose‑induced vascular endothelial cell dysfunction through the microRNA‑218/roundabout 1 and microRNA‑218/heme oxygenase‑1 axes

et al. 2018

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“…miR-218 reduces VEGF expression in prostate cancer by acting on the mTOR component RICTOR (rapamycin-insensitive companion of mammalian target of rapamycin) and by blocking the RICTOR/mTOR/HIF-1/VEGF signaling pathway [110]. In gastric cancer, two targets of miR-218 have been identified: Angiopoietin-2 and ROBO1 (roundabout guidance receptor 1); their downregulation results in a reduction of tumor proliferation, invasion, and angiogenesis [111,112]. Moreover, low levels of miR-218 result in endothelial cell sprouting, motility, and tube formation [112].…”

Section: Mir-218mentioning

confidence: 99%

“…In gastric cancer, two targets of miR-218 have been identified: Angiopoietin-2 and ROBO1 (roundabout guidance receptor 1); their downregulation results in a reduction of tumor proliferation, invasion, and angiogenesis [111,112]. Moreover, low levels of miR-218 result in endothelial cell sprouting, motility, and tube formation [112].…”

Section: Mir-218mentioning

confidence: 99%

Insights into the Regulation of Tumor Angiogenesis by Micro-RNAs

Leone

Buonavoglia

Fasano

et al. 2019

JCM

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One of the hallmarks of cancer is angiogenesis, a series of events leading to the formation of the abnormal vascular network required for tumor growth, development, progression, and metastasis. MicroRNAs (miRNAs) are short, single-stranded, non-coding RNAs whose functions include modulation of the expression of pro- and anti-angiogenic factors and regulation of the function of vascular endothelial cells. Vascular-associated microRNAs can be either pro- or anti-angiogenic. In cancer, miRNA expression levels are deregulated and typically vary during tumor progression. Experimental data indicate that the tumor phenotype can be modified by targeting miRNA expression. Based on these observations, miRNAs may be promising targets for the development of novel anti-angiogenic therapies. This review discusses the role of various miRNAs and their targets in tumor angiogenesis, describes the strategies and challenges of miRNA-based anti-angiogenic therapies and explores the potential use of miRNAs as biomarkers for anti-angiogenic therapy response.

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Tumor suppressor microRNAs in lung cancer: An insight to signaling pathways and drug resistance

Asghariazar

Sakhinia

Mansoori

et al. 2019

J of Cellular Biochemistry

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Lung cancer (LC) is the second common cancer for both women and men all over the world. Unfortunately, the number of LC deaths is increasing rapidly each year so early diagnosis of LC can be lifesaving. MicroRNAs are involved in multiple processes, such as cell differentiation, transcription, inflammation, proliferation, cell signaling, and apoptosis. In LC, microRNAs function as tumor suppressors (TS) or oncogenes depending on the targets. Changes in microRNAs expression level are related to tumor initiation, progression, and metastasis. MicroRNAs can regulate gene expression and thus affect the activity status of different signaling pathways including AKT, JAK-STAT, MAPK, TGF-β, WNT, and ERK signaling pathways. Positive or negative effects on drug resistance of LC are directly affected by microRNAs and their target genes. MicroRNAs can be beneficial in combination therapy with other drugs and chemotherapeutic agents for LC. K E Y W O R D S drug resistance, lung cancer, microRNA, signaling pathways, tumor suppressor How to cite this article: Asghariazar V, Sakhinia E, Mansoori B, Mohammadi A, Baradaran B. Tumor suppressor microRNAs in lung cancer: An insight to signaling pathways and drug resistance.

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miR-218 inhibited tumor angiogenesis by targeting ROBO1 in gastric cancer

Cited by 48 publications

References 32 publications

Human circular RNA‑0054633 regulates high glucose‑induced vascular endothelial cell dysfunction through the microRNA‑218/roundabout 1 and microRNA‑218/heme oxygenase‑1 axes

Human circular RNA‑0054633 regulates high glucose‑induced vascular endothelial cell dysfunction through the microRNA‑218/roundabout 1 and microRNA‑218/heme oxygenase‑1 axes

Insights into the Regulation of Tumor Angiogenesis by Micro-RNAs

Tumor suppressor microRNAs in lung cancer: An insight to signaling pathways and drug resistance

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