miR‑217‑5p regulates myogenesis in skeletal muscle stem cells by targeting FGFR2

Abstract

has been implicated in cell proliferation; however, its role in skeletal muscle stem cells (SkMScs) remains unknown. The present study aimed to explore the roles of mir-217-5p in the biological characteristics of SkMSCs. SkMSCs were identified by cell surface markers using flow cytometry. The present study observed that mir-217-5p mimics accelerated the proliferation and suppressed the differentiation in SkMScs. in addition, the results of the present study revealed that fibroblast growth factor receptor 2 (FGFr2) was a target of mir-217-5p, as mir-217-5p bound directly to the 3'-untranslated region of FGFr2 mrna, resulting in increased FGFr2 mrna and protein levels. in addition, the present study suppressed the expression of FGFr2 in SkMScs using a selective FGFr inhibitor AZD4547 and detected the efficiency of inhibition by reverse transcription-quantitative Pcr and western blotting. mir-217-5p levels were positively associated with FGFr2 expression, which was upregulated and accelerated the proliferation of SkMScs compared with that of the mir-nc group. collectively, these results demonstrated that mir-217-5p may act as a myogenesis promoter in SkMScs by directly targeting FGFr2 and may regulate the myogenesis of these cells.

Identification and characterization of circular RNAs in Longissimus dorsi muscle tissue from two goat breeds using RNA-Seq

“…It was therefore inferred that the up-regulated expression of circ_008092 in Longissimus dorsi muscle of LC goats may be responsible for its higher carcass weight and intramuscular fat content. Our target miRNAs prediction also found that circ_003628 would sponge either miR-217-5p positively related to skeletal muscle cell proliferation [49], or miRNAs positively related to skeletal muscle cell differentiation, including miR-1 [50], miR-26a [51], miR-27b [52] and miR-148a-3p [53]. These suggests that the lower expression of circ_003628 in LC goats may contribute to its higher meat production performance by less inhibition of the expression levels of these important miRNAs.…”

Deep Small RNA Sequencing Reveals Important miRNAs Related to Muscle Development and Intramuscular Fat Deposition in Longissimus dorsi Muscle From Different Goat Breeds

“…The miR-127-3p has been reported to inhibit proliferation of C2C12 myoblast ( 50 ), as well as proliferation and differentiation of porcine skeletal muscle satellite cells ( 51 ). Similarly, miR-217-5p and miR-487b-3p inhibited the differentiation of skeletal muscle cells in rats ( 52 ) and mice ( 53 ), respectively. Additionally, the inhibition of miR-410-3p on the differentiation of adipocyte by targeting IRS-1 has also been reported in humans ( 54 ).…”

Identification of Crucial Circrnas in Skeletal Muscle During Chicken Embryonic Development

“…Fibroblast Growth Factor Receptor 2 (FGFR2) was a member of FGFRs, which are a family of receptor tyrosine kinases expressed on the cell membrane that play crucial roles in both developmental and adult cells [42]. FGFR2 has been proved to regulate the myogenesis of skeletal muscle [43,44]. ACTN2 was highly expressed in muscle where it acted as a major structural component of the contractile apparatus at the Z-line [45].…”