miR-216a-5p acts as an oncogene in renal cell carcinoma

Chen, Peijie; Quan, Jing; Jin, Lü; Lin, Cheng-Yuan; Xu, Weijie; Xu, Jinling; Guan, Xin; Chen, Zebo; Ni, Liangchao; Yang, Shangqi; Chen, Yun; Lai, Yongqing

doi:10.3892/etm.2018.5881

Cited by 15 publications

(22 citation statements)

References 32 publications

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“…Our findings suggest that by repressing KIAA0101 protein, miR-216a-5p plays an essential role in suppressing the proliferation and metastasis of ESCC. Evidence reveals that the abnormal expression of miR-216a is involved in tumorigenesis and cancer progression (11)(12)(13)(14)(15)(16), but the specific role of miR-216a seems to be cell type-dependent. miR-216a has a low expression in NSCLC specimens and could inhibit cell activity of NSCLC (18), which was in accordance with observations in oral squamous cell carcinoma (31).…”

Section: Discussionmentioning

confidence: 99%

“…Currently, data are available on the miRNA signature of ESCC, and there are studies attempting to point out the potential value of miRNAs in ESCC screening, diagnosis, treatment, and prognosis (10). miR-216a-5p is a member of the miR-216 family (miR-216a/miR-216b), which has been shown to be dysregulated in several types of human cancers (11)(12)(13)(14)(15)(16). For example, miR-216a is significantly upregulated in hepatocellular carcinoma and was found to induce epithelial-mesenchymal transition (EMT) by suppressing the expression of PTEN, SMAD7, and TSLC1, thus contributing to carcinogenesis and recurrence (17).…”

Section: Microrna-216a-5p Suppresses Esophageal Squamous Cell Carcinomentioning

confidence: 99%

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MicroRNA‑216a‑5p suppresses esophageal squamous cell carcinoma progression by targeting KIAA0101

Sun

Yan

et al. 2020

Oncol Rep

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The KIAA0101 protein (also referred to as NS5ATP9 or Paf15) is overexpressed in esophageal squamous cell carcinoma (ESCC) and is associated with disease progression and poor patient survival, but how KIAA0101 expression is regulated remains unknown. The relationship between tumor miR-216a-5p expression and prognosis in patients with ESCC was revealed by survival analyses. Quantitative reverse-transcriptase PCR and western blot analysis were used to evaluate miR-216a-5p and KIAA0101 expression in human ESCC tissues and cell lines. The targeting of KIAA0101 by miR-216a-5p was verified by dual-luciferase reporter assays. The EC9706 and TE1 cell lines were transfected with miR-216a-5p mimics and inhibitor, or KIAA0101-specific shRNA and KIAA0101-expressing plasmids, in order to evaluate the effect of manipulating miR-216a-5p and KIAA0101 expression on ESCC cell proliferation, cell cycle progression, migration, and invasion. miR-216a-5p was lowly expressed and inversely correlated with KIAA0101 protein expression in ESCC tissues and cell lines. Lower miR-216a-5p expression was associated with worse prognosis in patients with ESCC. miR-216a-5p negatively regulated KIAA0101 expression by directly targeting the 3'-untranslated region of the KIAA0101 mRNA. Overexpression of miR-216a-5p suppressed the proliferation, migration, and invasion of the ESCC cell lines, whereas inhibition of miR-216a-5p had the opposite effects. Meanwhile, KIAA0101 promoted ESCC migration and invasion, and its overexpression abolished the antitumor effects of miR-216a-5p mimics. As a tumor suppressor, miR-216a-5p targets KIAA0101 to inhibit the proliferation, migration, and invasion of ESCC. Therefore, the miR-216a-5p/KIAA0101 axis may be a potential target for ESCC treatment.

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Section: Discussionmentioning

confidence: 99%

Section: Microrna-216a-5p Suppresses Esophageal Squamous Cell Carcinomentioning

confidence: 99%

MicroRNA‑216a‑5p suppresses esophageal squamous cell carcinoma progression by targeting KIAA0101

Sun

Yan

et al. 2020

Oncol Rep

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“…For example, miR-216a, which served as a predictor for poor prognosis of BLCA ( Figure. 6A), could promote the proliferation of renal cell carcinoma cell 50 . We also found HCP5, a lncRNA obtained from the target prediction of miR-216a, was correlated with immune checkpoints, which was reported by a recent literature 51 .…”

Section: Discussionmentioning

confidence: 99%

Construction of An Immune-Related CeRNA Network as a Prognostic Signature in Bladder Cancer

Zhou¹,

Tian²,

Yang³

et al. 2020

Preprint

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Background: Immunotherapy has been proved to be effective for bladder cancer (BLCA). However, the molecular network involved in BLCA tumor immune response remains unclear. This study aims to construct an immune-related ceRNA network and to identify the prognostic value. Methods: Based on The Cancer Genome Atlas (TCGA), we used single-sample gene set enrichment analysis (ssGSEA), weighted gene co-expression network analysis (WGCNA) to determine immune-related mRNA, lncRNA and miRNA. Then least absolute shrinkage, and selection operator (LASSO) and Cox regression were performed to identify the mRNAs with high prognostic value, and accordingly, the risk score was calculated. Internal and external validation were performed both in TCGA and GSE13507 with Kaplan-Meier (KM) survival and Receiver Operating Characteristic (ROC) curve analysis. Using the immune-related mRNA, lncRNA and miRNA, a ceRNA network was established via MiRcode, starBase, miRDB, miRTarBase and TargetScan. Besides, we also explore the relationship between the risk score and immune cell infiltration via CIBERSORT algorithm. Results: 5 mRNAs (PCGF3, FASN, DPYSL2, TGFBI and NTF3) were ultimately identified, and KM survival analysis displayed the 5-mRNA risk signature could predict the prognosis of BLCA with high efficacy both in TCGA (p = 1.006e-13) and GSE13507 (p = 7.759e-04). Using miRNA targeting molecular prediction database, an immune-related ceRNA network, including 5 mRNAs, 24 miRNAs and 86 lncRNAs, was constructed. Memory B cells, activated dendritic cells, and regulatory T cells infiltration into tumors were negatively correlated with risk score, while the infiltration levels of macrophages M0, M1 and M2 were positively correlated with risk score. Conclusion: This study helped to better understand the molecular mechanisms of tumor immune response from the view of ceRNA hypothesis, and provided a novel prognostic signature for bladder cancer.

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“…MicroRNAs (miRNAs), short (22 nucleotides in length) non-coding RNAs, involve in many biological processes (Jiang and Chen, 2012), such as differentiation of ordinary tissues and are important in the pathogenesis of lots of human cancers (Taucher et al, 2016). MiR-216a-5p, known as an oncogene, involved in the progression of many cancer subtypes (Chen et al, 2018). Chen et al has proved that miR-216a-5p elevates cell proliferation, activity and motility, and inhibits apoptosis (Chen et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…MiR-216a-5p, known as an oncogene, involved in the progression of many cancer subtypes (Chen et al, 2018). Chen et al has proved that miR-216a-5p elevates cell proliferation, activity and motility, and inhibits apoptosis (Chen et al, 2018). This finding demonstrates that miR-216a-5p has a positive effect on cell viability and anti-apoptosis.…”

Section: Introductionmentioning

confidence: 99%

Ginsenoside Rg1 defenses PC-12 cells against hydrogen peroxide-caused damage via up-regulation of miR-216a-5p

Liu

et al. 2019

Preprint

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BackgroundSpinal cord injury (SCI) is a destructive trauma accompanying with local injury, half of which cause chronic paralysis. Ginsenoside Rg1 exerts anti-apoptosis and anti-autophagy properties. Therefore, our goal was to study the protective mechanism of Rg1 in attenuating cell injury.MethodsMiR-216a-5p inhibitor was transfected into PC-12 cells, then cells were pre-treated by Rg1 and treated with 300 μM hydrogen peroxide (H2O2) for 24 h. CCK-8 and apoptosis experiments were done to test cell activity and apoptosis respectively. Expression of miR-216a-5p and cell damage relative factors was tested via qRT-PCR and western blot experiments, respectively.ResultsH2O2 induced cell activity suppression, apoptosis and autophagy well at the concentration of 300 μM, leading cell injury. Rg1 could attenuate cell injury induced by H2O2 at the working concentration of 200 μM that it elevated cell activity, attenuated apoptosis and autophagy and activated PI3K/AKT and AMPK signal pathways. Further, miR-216a-5p was up-regulated by Rg1. Rg1 played its role in relieving cell injury by positively regulating miR-216a-5p.ConclusionOur study demonstrated that Rg1 attenuated H2O2-caused cell injury through positively regulated miR-216a-5p.

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miR-216a-5p acts as an oncogene in renal cell carcinoma

Cited by 15 publications

References 32 publications

MicroRNA‑216a‑5p suppresses esophageal squamous cell carcinoma progression by targeting KIAA0101

MicroRNA‑216a‑5p suppresses esophageal squamous cell carcinoma progression by targeting KIAA0101

Construction of An Immune-Related CeRNA Network as a Prognostic Signature in Bladder Cancer

Ginsenoside Rg1 defenses PC-12 cells against hydrogen peroxide-caused damage via up-regulation of miR-216a-5p

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