miR‑216a‑3p inhibits osteogenic differentiation of human adipose‑derived stem cells via Wnt3a in the Wnt/β‑catenin signaling pathway

Liang, Daning; Song, Guodong; Zhang, Zhenning

doi:10.3892/etm.2022.11238

Cited by 4 publications

(1 citation statement)

References 30 publications

(28 reference statements)

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“…Moreover, by inhibiting the expression of Smad7, miR-195-5p promotes activation of the Wnt/β-catenin pathway, which is closely associated with osteogenic differentiation. Numerous studies have reported the involvement of the Wnt/β-catenin pathway in osteogenic differentiation [ 35 – 37 ], which we confirmed in the present study with respect to the osteogenic differentiation of VSMCs. However, despite demonstrating that miR-195-5p inhibits Smad7 and activates the Wnt/β-catenin pathway and osteogenic differentiation of VSMCs, the role of Smad7 in the miR-195-5p/Smad7/Wnt/β-catenin pathway axis has yet to be sufficiently established.…”

Section: Discussionsupporting

confidence: 90%

Mir-195-5p targets Smad7 regulation of the Wnt/β-catenin pathway to promote osteogenic differentiation of vascular smooth muscle cells

Lin,

Hou,

Tang

et al. 2024

BMC Cardiovasc Disord

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In this study, we sought to investigate the mechanisms of action of miR-195-5p in the osteogenic differentiation of vascular smooth muscle cells (VSMCs), and thereby provide novel insights and a reference for the targeted therapy of arterial media calcification. VSMC differentiation was induced using sodium β-glycerophosphate, and we investigated the effects of transfecting cells with miR-195-5p mimics, vectors overexpressing Smad7, and the Wnt/β-catenin pathway inhibitor (KYA1797K) on VSMC differentiation by determining cell viability and apoptosis, and the mRNA and protein expression of factors associated with osteogenic differentiation and the Wnt/β-catenin pathway. The results revealed that miR-195-5p mimics enhanced the osteogenic differentiation of VSMCs induced by β-glycerophosphate, whereas the overexpression of Smad7 reversed this phenomenon. In addition, KYA1797K was found to promote the effects of Smad7 overexpression. In conclusion, by targeting, Smad7, miR-195-5p promotes the Wnt/β-catenin pathway. and thus the osteogenic differentiation of VSMCs. These findings will provide a reference for elucidating the mechanisms whereby miR-195-5p regulates osteogenic differentiation.

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Section: Discussionsupporting

confidence: 90%

Mir-195-5p targets Smad7 regulation of the Wnt/β-catenin pathway to promote osteogenic differentiation of vascular smooth muscle cells

Lin,

Hou,

Tang

et al. 2024

BMC Cardiovasc Disord

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miR-708-3p targetedly regulates LSD1 to promote osteoblast differentiation of hPDLSCs in periodontitis

Shao,

Liu,

Zou

et al. 2024

Odontology

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Periodontitis (PD) is a multifactorial inflammatory disease associated with periodontopathic bacteria. Lysine-specific demethylase 1 (LSD1), a type of histone demethylase, has been implicated in the modulation of the inflammatory response process in oral diseases by binding to miRNA targets. This study investigates the molecular mechanisms by which miRNA binds to LSD1 and its subsequent effect on osteogenic differentiation. First, human periodontal ligament stem cells (hPDLSCs) were isolated, cultured, and characterized. These cells were then subjected to lipopolysaccharide (LPS) treatment to induce inflammation, after which osteogenic differentiation was initiated. qPCR and western blot were employed to monitor changes in LSD1 expression. Subsequently, LSD1 was silenced in hPDLSCs to evaluate its impact on osteogenic differentiation. Through bioinformatics and dual luciferase reporter assay, miR-708-3p was predicted and confirmed as a target miRNA of LSD1. Subsequently, miR-708-3p expression was assessed, and its role in hPDLSCs in PD was evaluated through overexpression. Using chromatin immunoprecipitation (ChIP) and western blot assay, we explored the potential regulation of osterix (OSX) transcription by miR-708-3p and LSD1 via di-methylated H3K4 (H3K4me2). Finally, we investigated the role of OSX in hPDLSCs. Following LPS treatment of hPDLSCs, the expression of LSD1 increased, but this trend was reversed upon the induction of osteogenic differentiation. Silencing LSD1 strengthened the osteogenic differentiation of hPDLSCs. miR-708-3p was found to directly bind to and negatively regulate LSD1, leading to the repression of OSX transcription through demethylation of H3K4me2. Moreover, overexpression of miR-708-3p was found to promote hPDLSCs osteogenic differentiation in inflammatory microenvironment. However, the protective effect was partially attenuated by reduced expression of OSX. Our findings indicate that miR-708-3p targetedly regulates LSD1 to enhance OSX transcription via H3K4me2 methylation, ultimately promoting hPDLSCs osteogenic differentiation.

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Unravelling microRNA regulation and miRNA–mRNA regulatory networks in osteogenesis driven by 3D nanotopographical cues

Balachander,

Nilawar,

Meka

et al. 2024

Biomater. Sci.

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miR‑216a‑3p inhibits osteogenic differentiation of human adipose‑derived stem cells via Wnt3a in the Wnt/β‑catenin signaling pathway

Cited by 4 publications

References 30 publications

Mir-195-5p targets Smad7 regulation of the Wnt/β-catenin pathway to promote osteogenic differentiation of vascular smooth muscle cells

Mir-195-5p targets Smad7 regulation of the Wnt/β-catenin pathway to promote osteogenic differentiation of vascular smooth muscle cells

miR-708-3p targetedly regulates LSD1 to promote osteoblast differentiation of hPDLSCs in periodontitis

Unravelling microRNA regulation and miRNA–mRNA regulatory networks in osteogenesis driven by 3D nanotopographical cues

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