miR-214 suppresses the osteogenic differentiation of bone marrow-derived mesenchymal stem cells and these effects are mediated through the inhibition of the JNK and p38 pathways

Guo, Yongkang; Li, Lianhua; Gao, Jie; Chen, Xiaobin; Sang, Qing-Hua

doi:10.3892/ijmm.2016.2826

Cited by 57 publications

(41 citation statements)

References 24 publications

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“…It has been argued that a single miRNA can simultaneously regulate the expression of multiple genes, and one gene can be regulated by multiple miRNAs [66]. miR-214 has been previously implicated in fibroblast differentiation of adipose-derived mesenchymal stem cells by targeting MFN2 [67], and regulates the differentiation of human hair follicle stem cells and bone marrow-derived mesenchymal stem cells [68,69]. ACLY, the putative target gene of miR-214, connects glucose metabolism to de novo synthesis of lipids [70].…”

Section: Discussionmentioning

confidence: 99%

MiRNAs and mRNAs Analysis during Abdominal Preadipocyte Differentiation in Chickens

Sun

Zhu

et al. 2020

Animals

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The excessive deposition of abdominal fat has become an important factor in restricting the production efficiency of chickens, so reducing abdominal fat deposition is important for improving growth rate. It has been proven that miRNAs play an important role in regulating many physiological processes of organisms. In this study, we constructed a model of adipogenesis by isolating preadipocytes (Ab-Pre) derived from abdominal adipose tissue and differentiated adipocytes (Ab-Ad) in vitro. Deep sequencing of miRNAs and mRNAs expressed in Ab-Pre and Ab-Ad groups was conducted to explore the effect of miRNAs and mRNAs on fat deposition. We identified 80 differentially expressed miRNAs (DEMs) candidates, 58 of which were up-regulated and 22 down-regulated. Furthermore, six miRNAs and six mRNAs were verified by qRT-PCR, and the results showed that the expression of the DEMs and differentially expressed genes (DEGs) in the two groups was consistent with our sequencing results. When target genes of miRNA were combined with mRNA transcriptome data, a total of 891 intersection genes were obtained, we predicted the signal pathways of cross genes enrichment to the MAPK signal pathway, insulin signal pathway, fatty acid metabolism, and ECM–receptor interaction. Meanwhile, we constructed miRNA and negatively correlated mRNA target networks, including 12 miRNA–mRNAs pairs, which showed a strong association with the abdominal adipocyte differentiation (miR-214−ACSBG2, NFKB2, CAMK2A, ACLY, CCND3, PLK3, ITGB2; miR-148a-5p−ROCK2; miR-10a-5p−ELOVL5; miR-146b-5p−LAMA4; miR-6615-5p−FLNB; miR-1774−COL6A1). Overall, these findings provide a background for further research on lipid metabolism. Thus, we can better understand the molecular genetic mechanism of chicken abdominal fat deposition.

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Section: Discussionmentioning

confidence: 99%

MiRNAs and mRNAs Analysis during Abdominal Preadipocyte Differentiation in Chickens

Sun

Zhu

et al. 2020

Animals

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“…These results suggest that miR‐199/214 maintained the cellular redox status and suppressed the activation of the p38 pathways. The overexpression of miR‐214 suppressed the phosphorylation of p38 in bone marrow‐derived mesenchymal stem cells, 51 while it increased the cellular proliferation and phosphorylation of ERK induced by isoproterenol (β‐adrenergic receptor agonist) in cardiac fibroblast 52 . Furthermore, the absence of p38α MAP kinase, which was phosphorylated by isoproterenol, in cardiac fibroblast suppressed the isoproterenol‐induced elevation of miR‐214 53 .…”

Section: Discussionmentioning

confidence: 99%

Overexpression of miR‐199/214 is a distinctive feature of iron‐induced and asbestos‐induced sarcomatoid mesothelioma in rats

et al. 2020

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Malignant mesothelioma (MM) is one of the most lethal tumors in humans. The onset of MM is linked to exposure to asbestos, which generates reactive oxygen species (ROS). ROS are believed to be derived from the frustrated phagocytosis and the iron in asbestos. To explore the pathogenesis of MM, peritoneal MM was induced in rats by the repeated intraperitoneal injection of iron saccharate and nitrilotriacetate. In the present study, we used microarray techniques to screen the microRNA (miR) expression profiles of these MM. We observed that the histological subtype impacted the hierarchical clustering of miR expression profiles and determined that miR‐199/214 is a distinctive feature of iron saccharate‐induced sarcomatoid mesothelioma (SM). Twist1, a transcriptional regulator of the epithelial‐mesenchymal transition, has been shown to activate miR‐199/214 transcription; thus, the expression level of Twist1 was examined in iron‐induced and asbestos‐induced mesotheliomas in rats. Twist1 was exclusively expressed in iron saccharate‐induced SM but not in the epithelioid subtype. The Twist1‐miR‐199/214 axis is activated in iron saccharate‐induced and asbestos‐induced SM. The expression levels of miR‐214 and Twist1 were correlated in an asbestos‐induced MM cell line, suggesting that the Twist1‐miR‐199/214 axis is preserved. MeT5A, an immortalized human mesothelial cell line, was used for the functional analysis of miR. The overexpression of miR‐199/214 promoted cellular proliferation, mobility and phosphorylation of Akt and ERK in MeT5A cells. These results indicate that miR‐199/214 may affect the aggressive biological behavior of SM.

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“…Significantly, miR-214 level was reported to vary with natural age in human osteoporotic bone specimens and increase in Alp + cells isolated from bones after 28 days hind-limb unloading, which implied that miR-214 might be potentially sensitive to mechanical unloading and played a crucial role in bone formation [ 85 ]. Furthermore, miR-214 has been shown to repress osteogenic differentiation or bone formation in different osteogenic cells through binding to various protein coding genes, such as Osterix in C2C12 myoblast cells [ 92 ], activating transcription factor 4 ( ATF4 ) in osteoblasts [ 85 ], and FGF in BMSCs [ 93 ]. The roles of mechanosensitive miRNAs in bone formation were showed in Table 5 .…”

Section: Mechanosensitive Mirnas and Bone Formationmentioning

confidence: 99%

Mechanosensitive miRNAs and Bone Formation

Chen

Zhang

Liang

et al. 2017

IJMS

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Mechanical stimuli are required for the maintenance of skeletal integrity and bone mass. An increasing amount of evidence indicates that multiple regulators (e.g., hormone, cytoskeleton proteins and signaling pathways) are involved in the mechanical stimuli modulating the activities of osteogenic cells and the process of bone formation. Significantly, recent studies have showed that several microRNAs (miRNAs) were sensitive to various mechanical stimuli and played a crucial role in osteogenic differentiation and bone formation. However, the functional roles and further mechanisms of mechanosensitive miRNAs in bone formation are not yet completely understood. This review highlights the roles of mechanosensitive miRNAs in osteogenic differentiation and bone formation and underlines their potential therapeutic application for bone loss induced by the altering of mechanical stimuli.

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miR-214 suppresses the osteogenic differentiation of bone marrow-derived mesenchymal stem cells and these effects are mediated through the inhibition of the JNK and p38 pathways

Cited by 57 publications

References 24 publications

MiRNAs and mRNAs Analysis during Abdominal Preadipocyte Differentiation in Chickens

MiRNAs and mRNAs Analysis during Abdominal Preadipocyte Differentiation in Chickens

Overexpression of miR‐199/214 is a distinctive feature of iron‐induced and asbestos‐induced sarcomatoid mesothelioma in rats

Mechanosensitive miRNAs and Bone Formation

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