MiR-214 Mediates Cell Proliferation and Apoptosis of Nasopharyngeal Carcinoma Through Targeting Both WWOX and PTEN

Han, Jing; Huang, Mao‐Ling; Li, Fen; Yang, Rui; Chen, Shiming; Tao, Zezhang

doi:10.1089/cbr.2019.2978

Cited by 15 publications

(15 citation statements)

References 46 publications

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“…In the present study, bioinformatics analysis revealed that the 3′‐UTR of PTEN harbors putative miR‐214 binding sites, and subsequent experiments confirmed that PTEN is a direct downstream target of miR‐214, which is consistent with the findings of a previous study 37 . Another recent study by Han et al 38 . suggested that miR‐214 promoted the proliferation and restrained the apoptosis of NPC cells through inhibiting the expression of both WW domain‐containing oxidoreductase and PTEN.…”

Section: Discussionsupporting

confidence: 91%

Circular RNA ITCH attenuates the progression of nasopharyngeal carcinoma by inducing PTEN upregulation via miR‐214

Wang

Sang

Zhang

et al. 2021

The Journal of Gene Medicine

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Background Circular RNA itchy E3 ubiquitin protein ligase (circ‐ITCH) has previously been reported to play a key role in carcinogenesis. Nevertheless, the role of circ‐ITCH in nasopharyngeal carcinoma (NPC) remains to be explored. Methods Gene expression analysis was performed using a quantitative real‐time polymerase chain reaction, western blotting and immunohistochemistry. The role of circ‐ITCH in NPC was explored using 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl tetrazolium bromide, colony formation, transwell invasion, scratch healing and xenograft tumor assays. Furthermore, luciferase reporter assay was carried out to assess the interactions among circ‐ITCH, microRNA‐214 (miR‐214) and phosphatase and tensin homolog (PTEN). Results The levels of circ‐ITCH and PTEN were decreased, whereas the level of miR‐214 was increased in NPC tissues collected from 28 subjects compared to normal nasopharynx tissues collected from 15 subjects. Moreover, a negative correlation between circ‐ITCH and miR‐214 expression and a positive correlation between circ‐ITCH and PTEN expression were observed in NPC tissues. Downregulation of circ‐ITCH expression was also observed in NPC cell lines. In addition, upregulation of circ‐ITCH markedly inhibited NPC cell proliferation, migration and invasion. Furthermore, circ‐ITCH was confirmed to exert its function by sponging miR‐214. PTEN was found to be a direct target gene of miR‐214 and its expression was negatively correlated with miR‐214 expression in NPC tissues. Moreover, our results showed that the circ‐ITCH/miR‐214 axis regulated NPC proliferation, migration and invasion through regulating the expression of PTEN. Upregulation of circ‐ITCH or PTEN blocked miR‐214‐mediated promotion of NPC tumorigenesis in vitro. Additionally, upregulation of circ‐ITCH also suppressed NPC tumorigenesis in vivo. Conclusions The present study demonstrated that circ‐ITCH suppressed NPC tumorigenesis by upregulating PTEN expression through interacting with miR‐214, thus proposing a novel mechanism for NPC inhibition.

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Section: Discussionsupporting

confidence: 91%

Circular RNA ITCH attenuates the progression of nasopharyngeal carcinoma by inducing PTEN upregulation via miR‐214

Wang

Sang

Zhang

et al. 2021

The Journal of Gene Medicine

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“…For example, miR-222 increases the invasion of ovarian cancer cells by acting on the target gene PTEN [14]. MiR-214 regulates the proliferation and metastasis of thyroid tumor cells by acting on WWOX and PTEN [15]. MiR-543 accelerates the proliferation of nasopharyngeal carcinoma cells by targeting JAM-A [16].…”

Section: Discussionmentioning

confidence: 99%

Serum exosomal miR-34a as a potential biomarker for the diagnosis and prognostic of hepatocellular carcinoma

Chen¹,

Mao²,

Chen³

et al. 2022

J. Cancer

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Background: Circulating exosomal microRNAs (miRNAs) are considered as potentially non-invasive biomarkers for early detection and prognosis of cancers. Due to the lack of highly sensitive and specific molecular markers, a lot of patients with hepatocellular carcinoma are diagnosed in advanced stages. This study aims to explore the expression mode and clinical detection value of serum exosomal miR-34a in HCC, providing new potential targets and theoretical basis for the early diagnosis and prognosis monitoring of hepatocellular carcinoma. Methods: The expression of serum exosomal miR-34a in 60 HCC patients before and after operation and 60 healthy examiners was abstracted and detected by ultracentrifugation and real-time quantitative PCR. Using ROC analysis, Kaplan-Meier survival analysis and Cox regression analysis, the value of serum exosomal miR-34a on diagnosis and prognosis in HCC patients was assessed.Results: The expression level of serum exosomal miR-34a in preoperative patients was reduced significantly comparing with that in healthy examiners and postoperative patients (P<0.01; P<0.05). Moreover, the decrease of serum exosomal miR-34a was correlated significantly with differentiation degree, TNM stage, tumor infiltration depth and lymph node metastasis(P<0.05), but had no statistical differences with gender, age, ALT, AST, viral infection, cirrhosis and tumor size of HCC patients (P>0.05). At the same time, the combination of serum exosomal miR-34a and α-fetoprotein (AFP) showed high capability on diagnosis to distinguish healthy examiners and HCC patients through ROC analysis. The overall survival of patients with lower expression of serum exosomal miR-34a was worse than that of patients with high level expression by Kaplan-Meier survival analysis (P<0.05). Univariate and multivariate Cox regression analysis both showed that serum exosomal miR-34a was independently related to OS. Conclusions: Collectively, serum exosomal miR-34a is significantly down-regulated in HCC patients and might be a novel noninvasive biomarker for diagnosis and prognosis of HCC.

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“…For example, miR-214 can inhibit the activity of hepatocellular carcinoma by targeting E2F3 [ 18 ]. In nasopharyngeal carcinoma, miR-214 can activate Akt signal pathway by regulating PTEN, so as to promote the proliferation activity of nasopharyngeal carcinoma cells [ 19 ]. Relevant studies have shown that miR-214 level increases significantly in a variety of tumors and can regulate Wnt/ β -catenin signaling pathway to promote cancer cell growth [ 20 – 22 ].…”

Section: Discussionmentioning

confidence: 99%

miR-214 Modulates the Growth and Migration of Oral Cancer before and after Chemotherapy through Mediating ULK1

Yang

Sun

et al. 2022

Journal of Immunology Research

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The role of miRNAs as crucial components in carcinogenesis has been well documented. However, whether and how miR-214 influences oral cancer cells’ drug resistance remains to be elucidated, and its downstream targets are still under investigation. Hence, this research is aimed at determining miR-214 and ULK1 expression in oral cancer before and after chemotherapy and their correlations with cancer cell growth. Human oral normal epithelial cells and human tongue squamous cell carcinoma CAL-27 cells were cultured to detect miR-214 and ULK1 levels. It was found that before chemotherapy, miR-214 was higher, while ULK1 was underexpressed in CAL-27 cells, versus normal epithelial cells. After chemotherapy, miR-214 decreased obviously in CAL-27 cells, while ULK1 level increased significantly. In addition, autophagy-related genes (Beclin 1, mTOR, and P53) in CAL-27 cells were found to be significantly inhibited before chemotherapy and were obviously increased after chemotherapy. Moreover, to further determine the impacts of miR-214 and ULK1 on oral cancer cell growth after chemotherapy, the two were overexpressed or silenced in CAL-27 cells after transfection. We found that ULK1 could effectively decrease the activity and invasion of CAL-27 cells and increase their apoptosis level, while miR-214 could antagonize its antitumor effect. Therefore, miR-214 can be used as an early prognostic biomarker for oral cancer, and ULK1 is a new candidate therapeutic target.

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MiR-214 Mediates Cell Proliferation and Apoptosis of Nasopharyngeal Carcinoma Through Targeting Both WWOX and PTEN

Cited by 15 publications

References 46 publications

Circular RNA ITCH attenuates the progression of nasopharyngeal carcinoma by inducing PTEN upregulation via miR‐214

Circular RNA ITCH attenuates the progression of nasopharyngeal carcinoma by inducing PTEN upregulation via miR‐214

Serum exosomal miR-34a as a potential biomarker for the diagnosis and prognostic of hepatocellular carcinoma

miR-214 Modulates the Growth and Migration of Oral Cancer before and after Chemotherapy through Mediating ULK1

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